RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Human respiratory syncytial virus A (strain A2)

[TaxID: 11259]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Paramyxoviridae; Pneumovirinae; Pneumovirus.

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1016/0042-6822(91)90140-7; PubMed=2053282 [NCBI, ExPASy, EBI, Israel, Japan]
Stec D.S., Hill M.G. III, Collins P.L.;
"Sequence analysis of the polymerase L gene of human respiratory syncytial virus and predicted phylogeny of nonsegmented negative-strand viruses.";
Virology 183:273-287(1991).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein (By similarity).
SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
SIMILARITY: Belongs to the paramyxoviruses L protein family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M75730; AAA47418.1; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A40317; RRNZA2.

3D structure databases

ModBase

P28887.

Family and domain databases

InterPro

IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2165`	`2165`	`Large structural protein.`	`PRO_0000142727`
`DOMAIN`	`693 877`	`185`	`RdRp catalytic.`

Sequence information

Length: 2165 AA [This is the length of the unprocessed precursor]

Molecular weight: 250386 Da [This is the MW of the unprocessed precursor]

CRC64: 5A3DCA0805305197 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDPIINGNSA NVYLTDSYLK GVISFSECNA LGSYIFNGPY LKNDYTNLIS RQNPLIEHMN 

        70         80         90        100        110        120 
LKKLNITQSL ISKYHKGEIK LEEPTYFQSL LMTYKSMTSS EQIATTNLLK KIIRRAIEIS 

       130        140        150        160        170        180 
DVKVYAILNK LGLKEKDKIK SNNGQDEDNS VITTIIKDDI LSAVKDNQSH LKADKNHSTK 

       190        200        210        220        230        240 
QKDTIKTTLL KKLMCSMQHP PSWLIHWFNL YTKLNNILTQ YRSNEVKNHG FTLIDNQTLS 

       250        260        270        280        290        300 
GFQFILNQYG CIVYHKELKR ITVTTYNQFL TWKDISLSRL NVCLITWISN CLNTLNKSLG 

       310        320        330        340        350        360 
LRCGFNNVIL TQLFLYGDCI LKLFHNEGFY IIKEVEGFIM SLILNITEED QFRKRFYNSM 

       370        380        390        400        410        420 
LNNITDAANK AQKNLLSRVC HTLLDKTVSD NIINGRWIIL LSKFLKLIKL AGDNNLNNLS 

       430        440        450        460        470        480 
ELYFLFRIFG HPMVDERQAM DAVKINCNET KFYLLSSLSM LRGAFIYRII KGFVNNYNRW 

       490        500        510        520        530        540 
PTLRNAIVLP LRWLTYYKLN TYPSLLELTE RDLIVLSGLR FYREFRLPKK VDLEMIINDK 

       550        560        570        580        590        600 
AISPPKNLIW TSFPRNYMPS HIQNYIEHEK LKFSESDKSR RVLEYYLRDN KFNECDLYNC 

       610        620        630        640        650        660 
VVNQSYLNNP NHVVSLTGKE RELSVGRMFA MQPGMFRQVQ ILAEKMIAEN ILQFFPESLT 

       670        680        690        700        710        720 
RYGDLELQKI LELKAGISNK SNRYNDNYNN YISKCSIITD LSKFNQAFRY ETSCICSDVL 

       730        740        750        760        770        780 
DELHGVQSLF SWLHLTIPHV TIICTYRHAP PYIGDHIVDL NNVDEQSGLY RYHMGGIEGW 

       790        800        810        820        830        840 
CQKLWTIEAI SLLDLISLKG KFSITALING DNQSIDISKP IRLMEGQTHA QADYLLALNS 

       850        860        870        880        890        900 
LKLLYKEYAG IGHKLKGTET YISRDMQFMS KTIQHNGVYY PASIKKVLRV GPWINTILDD 

       910        920        930        940        950        960 
FKVSLESIGS LTQELEYRGE SLLCSLIFRN VWLYNQIALQ LKNHALCNNK LYLDILKVLK 

       970        980        990       1000       1010       1020 
HLKTFFNLDN IDTALTLYMN LPMLFGGGDP NLLYRSFYRR TPDFLTEAIV HSVFILSYYT 

      1030       1040       1050       1060       1070       1080 
NHDLKDKLQD LSDDRLNKFL TCIITFDKNP NAEFVTLMRD PQALGSERQA KITSEINRLA 

      1090       1100       1110       1120       1130       1140 
VTEVLSTAPN KIFSKSAQHY TTTEIDLNDI MQNIEPTYPH GLRVVYESLP FYKAEKIVNL 

      1150       1160       1170       1180       1190       1200 
ISGTKSITNI LEKTSAIDLT DIDRATEMMR KNITLLIRIL PLDCNRDKRE ILSMENLSIT 

      1210       1220       1230       1240       1250       1260 
ELSKYVRERS WSLSNIVGVT SPSIMYTMDI KYTTSTISSG IIIEKYNVNS LTRGERGPTK 

      1270       1280       1290       1300       1310       1320 
PWVGSSTQEK KTMPVYNRQV LTKKQRDQID LLAKLDWVYA SIDNKDEFME ELSIGTLGLT 

      1330       1340       1350       1360       1370       1380 
YEKAKKLFPQ YLSVNYLHRL TVSSRPCEFP ASIPAYRTTN YHFDTSPINR ILTEKYGDED 

      1390       1400       1410       1420       1430       1440 
IDIVFQNCIS FGLSLMSVVE QFTNVCPNRI ILIPKLNEIH LMKPPIFTGD VDIHKLKQVI 

      1450       1460       1470       1480       1490       1500 
QKQHMFLPDK ISLTQYVELF LSNKTLKSGS HVNSNLILAH KISDYFHNTY ILSTNLAGHW 

      1510       1520       1530       1540       1550       1560 
ILIIQLMKDS KGIFEKDWGE GYITDHMFIN LKVFFNAYKT YLLCFHKGYG KAKLECDMNT 

      1570       1580       1590       1600       1610       1620 
SDLLCVLELI DSSYWKSMSK VFLEQKVIKY ILSQDASLHR VKGCHSFKLW FLKRLNVAEF 

      1630       1640       1650       1660       1670       1680 
TVCPWVVNID YHPTHMKAIL TYIDLVRMGL INIDRIHIKN KHKFNDEFYT SNLFYINYNF 

      1690       1700       1710       1720       1730       1740 
SDNTHLLTKH IRIANSELEN NYNKLYHPTP ETLENILANP IKSNDKKTLN DYCIGKNVDS 

      1750       1760       1770       1780       1790       1800 
IMLPLLSNKK LIKSSAMIRT NYSKQDLYNL FPMVVIDRII DHSGNTAKSN QLYTTTSHQI 

      1810       1820       1830       1840       1850       1860 
SLVHNSTSLY CMLPWHHINR FNFVFSSTGC KISIEYILKD LKIKDPNCIA FIGEGAGNLL 

      1870       1880       1890       1900       1910       1920 
LRTVVELHPD IRYIYRSLKD CNDHSLPIEF LRLYNGHINI DYGENLTIPA TDATNNIHWS 

      1930       1940       1950       1960       1970       1980 
YLHIKFAEPI SLFVCDAELS VTVNWSKIII EWSKHVRKCK YCSSVNKCML IVKYHAQDDI 

      1990       2000       2010       2020       2030       2040 
DFKLDNITIL KTYVCLGSKL KGSEVYLVLT IGPANIFPVF NVVQNAKLIL SRTKNFIMPK 

      2050       2060       2070       2080       2090       2100 
KADKESIDAN IKSLIPFLCY PITKKGINTA LSKLKSVVSG DILSYSIAGR NEVFSNKLIN 

      2110       2120       2130       2140       2150       2160 
HKHMNILKWF NHVLNFRSTE LNYNHLYMVE STYPYLSELL NSLTTNELKK LIKITGSLLY 


NFHNE

P28887 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools