[1]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
DOI=10.1021/bi00246a008; PubMed=1868068 [NCBI, ExPASy, EBI, Israel, Japan]
Mischak H.,
Bodenteich A.,
Kolch W.,
Goodnight J.,
Hofer F.,
Mushinski J.F.;
"Mouse protein kinase C-delta, the major isoform expressed in mouse hemopoietic cells: sequence of the cDNA, expression patterns, and characterization of the protein.";
Biochemistry 30:7925-7931(1991).
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[2]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
STRAIN=ICR;
TISSUE=Brain;
PubMed=1765103 [NCBI, ExPASy, EBI, Israel, Japan]
Mizuno K.,
Kubo K.,
Saido T.C.,
Akita Y.,
Osada S.,
Kuroki T.,
Ohno S.,
Suzuki K.;
"Structure and properties of a ubiquitously expressed protein kinase C, nPKC delta.";
Eur. J. Biochem. 202:931-940(1991).
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[3]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
STRAIN=129/SvJ;
Wheeler D.L.,
Gillis M.E.,
Verma A.K.;
"Intron/exon structure of the murine protein kinase C delta gene.";
Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
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[4]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
DOI=10.1248/bpb.24.973; PubMed=11558579 [NCBI, ExPASy, EBI, Israel, Japan]
Sakurai Y.,
Onishi Y.,
Tanimoto Y.,
Kizaki H.;
"Novel protein kinase C delta isoform insensitive to caspase-3.";
Biol. Pharm. Bull. 24:973-977(2001).
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[5]
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FUNCTION.
DOI=10.1038/416860a; PubMed=11976686 [NCBI, ExPASy, EBI, Israel, Japan]
Mecklenbraeuker I.,
Saijo K.,
Zheng N.Y.,
Leitges M.,
Tarakhovsky A.;
"Protein kinase Cdelta controls self-antigen-induced B-cell tolerance.";
Nature 416:860-865(2002).
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[6]
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PHOSPHORYLATION AT THR-505.
DOI=10.1126/science.281.5385.2042; PubMed=9748166 [NCBI, ExPASy, EBI, Israel, Japan]
Le Good J.A.,
Ziegler W.H.,
Parekh D.B.,
Alessi D.R.,
Cohen P.,
Parker P.J.;
"Protein kinase C isotypes controlled by phosphoinositide 3-kinase through the protein kinase PDK1.";
Science 281:2042-2045(1998).
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[7]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-311, AND MASS SPECTROMETRY.
TISSUE=Mast cell;
PubMed=17947660 [NCBI, ExPASy, EBI, Israel, Japan]
Cao L.,
Yu K.,
Banh C.,
Nguyen V.,
Ritz A.,
Raphael B.J.,
Kawakami Y.,
Kawakami T.,
Salomon A.R.;
"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling.";
J. Immunol. 179:5864-5876(2007).
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[8]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-311, AND MASS SPECTROMETRY.
TISSUE=Brain;
DOI=10.1021/pr0701254; PubMed=18034455 [NCBI, ExPASy, EBI, Israel, Japan]
Ballif B.A.,
Carey G.R.,
Sunyaev S.R.,
Gygi S.P.;
"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain.";
J. Proteome Res. 7:311-318(2008).
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[9]
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X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 231-280 IN COMPLEX WITH PHORBOL ESTER AND ZINC IONS.
DOI=10.1016/0092-8674(95)90011-X; PubMed=7781068 [NCBI, ExPASy, EBI, Israel, Japan]
Zhang G.,
Kazanietz M.G.,
Blumberg P.M.,
Hurley J.H.;
"Crystal structure of the cys2 activator-binding domain of protein kinase C delta in complex with phorbol ester.";
Cell 81:917-924(1995).
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- FUNCTION: This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin (By similarity).
- CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
- ENZYME REGULATION: Three specific sites; Thr-505 (activation loop of the kinase domain), Ser-643 (turn motif) and Ser-662 (hydrophobic region), need to be phosphorylated for its full activation.
- SUBUNIT: Interacts with RAD9A, CDCP1, PDK1 and MUC1 (By similarity).
- INTERACTION:
Q8WV44-2:TRIM41 (xeno); NbExp=1; IntAct=EBI-1551324, EBI-726015;
- SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane; Peripheral membrane protein (By similarity).
- ALTERNATIVE PRODUCTS:
2 named isoforms [FASTA] produced by alternative splicing.
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| Name | 2 |
| Synonyms | PKC-delta-II |
| Isoform ID | P28867-2 |
| Features which should be applied to build the isoform sequence: VSP_004741. |
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- TISSUE SPECIFICITY: Isoform 1 is highly expressed in developing pro- and pre-B-cells and moderately in mature T-cells. Isoform 2 is highly expressed in testis and ovary and at a lower level in thymocytes, brain and kidney.
- DOMAIN: The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.
- DOMAIN: The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner (By similarity).
- PTM: Phosphorylated on residue Thr-505, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-505 phosphorylation occurs it is not a prerequisite for enzymatic activity (By similarity).
- SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
- SIMILARITY: Contains 1 AGC-kinase C-terminal domain.
- SIMILARITY: Contains 1 C2 domain.
- SIMILARITY: Contains 2 phorbol-ester/DAG-type zinc fingers.
- SIMILARITY: Contains 1 protein kinase domain.
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