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[1]
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NUCLEOTIDE SEQUENCE [MRNA].
PubMed=2825726 [NCBI, ExPASy, EBI, Israel, Japan]
Fini M.E.,
Karmilowicz M.J.,
Ruby P.L.,
Beeman A.M.,
Borges K.A.,
Brinckerhoff C.E.;
"Cloning of a complementary DNA for rabbit proactivator. A metalloproteinase that activates synovial cell collagenase, shares homology with stromelysin and transin, and is coordinately regulated with collagenase.";
Arthritis Rheum. 30:1254-1264(1987).
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[2]
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NUCLEOTIDE SEQUENCE [MRNA] OF 1-167.
PubMed=3030290 [NCBI, ExPASy, EBI, Israel, Japan]
Whitham S.E.,
Murphy G.,
Angel P.,
Rahmsdorf H.J.,
Smith B.,
Lyons A.,
Harris T.J.R.,
Reynolds J.J.,
Herrlich P.,
Docherty A.J.P.;
"Comparison of human stromelysin and collagenase by cloning and sequence analysis.";
Biochem. J. 240:913-916(1986).
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- FUNCTION: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
- CATALYTIC ACTIVITY: Preferential cleavage where P1', P2' and P3' are hydrophobic residues.
- COFACTOR: Binds 4 calcium ions per subunit (By similarity).
- COFACTOR: Binds 2 zinc ions per subunit (By similarity).
- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (Probable).
- DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
- SIMILARITY: Belongs to the peptidase M10A family [view classification].
- SIMILARITY: Contains 4 hemopexin-like domains.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 478 AA [This is the length of the unprocessed precursor] |
Molecular weight: 53942 Da [This is the MW of the unprocessed precursor] |
CRC64: CA742E31A4549D40 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MKTLPTLLLL CVALCSAYPL DGASRDADTT NMDLLQQYLE NYYNLEKDVK QFVKRKDSSP
70 80 90 100 110 120
VVKKIQEMQK FLGLEVTGKL DSNTLEVIRK PRCGVPDVGH FSTFPGTPKW TKTHLTYRIV
130 140 150 160 170 180
NYTPDLPRDA VDAAIEKALK VWEEVTPLTF SRKYEGEADI MISFGVREHG DFIPFDGPGN
190 200 210 220 230 240
VLAHAYAPGP GINGDAHFDD DEQWTKDTTG TNLFLVAAHE LGHSLGLFHS ANPEALMYPV
250 260 270 280 290 300
YNAFTDLARF RLSQDDVDGI QSLYGPAPAS PDNSGVPMEP VPPGSGTPVM CDPDLSFDAI
310 320 330 340 350 360
STLRGEILFF KDRYFWRKSL RILEPEFHLI SSFWPSLPSA VDAAYEVISR DTVFIFKGTQ
370 380 390 400 410 420
FWAIRGNEVQ AGYPRSIHTL GFPSTIRKID AAISDKERKK TYFFVEDKYW RFDEKRQSLE
430 440 450 460 470
PGFPRHIAED FPGINPKIDA VFEAFGFFYF FSGSSQSEFD PNAKKVTHVL KSNSWFQC
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P28863 in FASTA format |
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ExPASy Home page
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