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UniProtKB/Swiss-Prot entry P28840

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NEC1_RAT
Primary accession number P28840
Secondary accession numbers None
Integrated into Swiss-Prot on December 1, 1992
Sequence was last modified on December 1, 1992 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 71)
Name and origin of the protein
Protein name Neuroendocrine convertase 1 [Precursor]
Synonyms NEC 1
EC 3.4.21.93
Prohormone convertase 1
Proprotein convertase 1
PC1
Gene name
Name: Pcsk1
Synonyms: Bdp, Nec-1, Nec1
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
PubMed=1791845 [NCBI, ExPASy, EBI, Israel, Japan]
Bloomquist B.T., Eipper B.A., Mains R.E.;
"Prohormone-converting enzymes: regulation and evaluation of function using antisense RNA.";
Mol. Endocrinol. 5:2014-2024(1991).
[2]
 NUCLEOTIDE SEQUENCE [MRNA].
PubMed=1954888 [NCBI, ExPASy, EBI, Israel, Japan]
Hakes D.J., Birch N.P., Mezey A., Dixon J.E.;
"Isolation of two complementary deoxyribonucleic acid clones from a rat insulinoma cell line based on similarities to Kex2 and furin sequences and the specific localization of each transcript to endocrine and neuroendocrine tissues in rats.";
Endocrinology 129:3053-3063(1991).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M76705; AAA40945.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M83745; AAA41476.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A41556; KXRTC1.
RefSeq NP_058787.1; -.
UniGene Rn.11384
3D structure databases
HSSP P21662; 1KN6. [HSSP ENTRY / PDB]
ModBase P28840.
Protein family/group databases
MEROPS S08.072; -.
Organism-specific databases
RGD 3272; Pcsk1.
Gene expression databases
ArrayExpress P28840; -.
GermOnline ENSRNOG00000011107; Rattus norvegicus.
Ontologies
GO
GO:0031410; Cellular component: cytoplasmic vesicle (inferred from electronic annotation from UniProtKB-KW).
GO:0005509; Molecular function: calcium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004252; Molecular function: serine-type endopeptidase activity (inferred from electronic annotation from InterPro).
GO:0006508; Biological process: proteolysis (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR000209; Pept_S8_S53.
IPR015500; Peptidase_S8_subtilisin-rel.
IPR002884; PrprotnconvertsP.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.200; Pept_S8_S53; 1.
PANTHER PTHR10795; SubtilSerProt; 1.
Pfam PF01483; P_proprotein; 1.
PF00082; Peptidase_S8; 1.
Pfam graphical view of domain structure.
PRINTS PR00723; SUBTILISIN.
ProDom PD000717; PrprotnconvertsP; 1.
[Domain structure / List of seq. sharing at least 1 domain]
PROSITE PS00136; SUBTILASE_ASP; 1.
PS00137; SUBTILASE_HIS; 1.
PS00138; SUBTILASE_SER; 1.
BLOCKS P28840.
ProtoNet P28840.
Genome annotation databases
Ensembl ENSRNOG00000011107; Rattus norvegicus. [Contig view]
GeneID 25204; -.
KEGG rno:25204; -.
Phylogenomic databases
HOVERGEN P28840; -.
Other
NextBio 605733; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Calcium; Cleavage on pair of basic residues; Cytoplasmic vesicle; Glycoprotein; Hydrolase; Protease; Serine protease; Signal; Zymogen.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    27  27     Potential. 
PROPEP   28   110  83     Potential. PRO_0000027063
CHAIN   111   752  642     Neuroendocrine convertase 1. PRO_0000027064
REGION   122   410  289     Catalytic. 
REGION   739   751  13     Amphipathic (Potential). 
ACT_SITE   167   167        Charge relay system (By similarity). 
ACT_SITE   208   208        Charge relay system (By similarity). 
ACT_SITE   382   382        Charge relay system (By similarity). 
CARBOHYD   173   173        N-linked (GlcNAc...) (Potential). 
CARBOHYD   401   401        N-linked (GlcNAc...) (Potential). 
CARBOHYD   645   645        N-linked (GlcNAc...) (Potential). 
DISULFID   225   374        By similarity. 
DISULFID   317   347        By similarity. 
DISULFID   467   494        By similarity. 
CONFLICT   366   366        T -> TT (in Ref. 2; AAA41476). 
CONFLICT   514   514        E -> A (in Ref. 2; AAA41476). 
Sequence information
Length: 752 AA [This is the length of the unprocessed precursor] Molecular weight: 84121 Da [This is the MW of the unprocessed precursor] CRC64: F630AD830A076DED [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MKQRGWTLQC TAFTLFCVWC ALNSVKAKRQ FVNEWAAEIH GGPEAASAIA EELGYDLLGQ 

        70         80         90        100        110        120 
IGSLENHYLF KHKNHPRRSR RSALHITKRL SDDDRVIWAE QQYEKERRKR SVPRDSALNL 

       130        140        150        160        170        180 
FNDPMWNQQW YLQDTRMTAS LPKLDLHVIP VWQKGITGKG VVITVLDDGL EWNHTDIYAN 

       190        200        210        220        230        240 
YDPEASYDFN DNDHDPFPRY DPTNENKHGT RCAGEIAMQA NNHKCGVGVA YNSKVGGIRM 

       250        260        270        280        290        300 
LDGIVTDAIE ASSIGFNPGH VDIYSASWGP NDDGKTVEGP GRLAQKAFEY GVKQGRQGKG 

       310        320        330        340        350        360 
SIFVWASGNG GRQGDNCDCD GYTDSIYTIS ISSASQQGLS PWYAEKCSST LATSYSSGDY 

       370        380        390        400        410        420 
TDQRITSADL HNDCTETHTG TSASAPLAAG IFALALEANP NLTWRDMQHL VVWTSEYDPL 

       430        440        450        460        470        480 
ANNPGWKKNG AGLMVNSRFG FGLLNAKALV DLADPRTWRN VPEKKECIIK DNNFEPRALK 

       490        500        510        520        530        540 
ANGEVIVEIP TRACEGQENA INSLEHVQFE ATIEYSRRGD LHVTLTSAAG TSTVLLAERE 

       550        560        570        580        590        600 
RDTSPNGFKN WDFMSVHTWG ENPVGTWTLK VTDMSGRMQN EGRIVNWKLI LHGTSSQPEH 

       610        620        630        640        650        660 
MKQPRVYTSY NTVQNDRRGV EKMVNVVEEK PTQNSLNGNL LVPKNSSSSS VEDRRDEQVQ 

       670        680        690        700        710        720 
GAPSKAMLRL LQSAFSKNTP SKQSSKIPSA KLSVPYEGLY EALEKLNKPS QLEDSEDSLY 

       730        740        750 
SDYVDVFYNT KPYKHRDDRL LQALMDILNE KN
P28840 in FASTA format

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