PROTEIN SEQUENCE, STRUCTURE OF CARBOHYDRATE, SULFATION AT TYR-61, AND MASS SPECTROMETRY.
DOI=10.1021/bi00123a012; PubMed=1540584 [NCBI, ExPASy, EBI, Israel, Japan]
Steiner V., Knecht R., Boernsen O., Gassmann E., Stone S.R., Raschdorf F., Schlaeppi J.-M., Maschler R.;
"Primary structure and function of novel O-glycosylated hirudins from the leech Hirudinaria manillensis.";
Biochemistry 31:2294-2298(1992).

Comments

FUNCTION: Hirudin is a potent thrombin-specific protease inhibitor. It forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen.
SUBCELLULAR LOCATION: Secreted.
PTM: O-linked glycan consists of Fuc-Gal-GalNAc trisaccharide.
SIMILARITY: Belongs to the protease inhibitor I14 (hirudin) family [view classification].

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

PIR

A42207; A42207.

3D structure databases

HSSP

P01050; 5HIR. [HSSP ENTRY / PDB]

ModBase

P28512.

PTM databases

GlycoSuiteDB

P28512; -.

Ontologies

GO:0005576;	Cellular component: extracellular region (inferred from electronic annotation from UniProtKB-KW).
GO:0004867;	Molecular function: serine-type endopeptidase inhibitor activity (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR000429; Prot_inh_hirudin.
Graphical view of domain structure.

Gene3D

G3DSA:2.70.10.10; Prot_inh_hirudin; 1.

Pfam

PF00713; Hirudin; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF001640; Hirudin; 1.

PRINTS

PR00777; HIRUDIN.

ProDom

PD004216; Prot_inh_hirudin; 1.
[Domain structure / List of seq. sharing at least 1 domain]

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; Glycoprotein; Protease inhibitor; Secreted; Serine protease inhibitor; Sulfation.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 63`	`63`	`Hirudin-P6.`	`PRO_0000195652`
`REGION`	`1 3`	`3`	`Interaction with thrombin active site (By similarity).`
`REGION`	`53 63`	`11`	`Interaction with fibrinogen-binding exosite of thrombin (By similarity).`
`MOD_RES`	`61 61`		`Sulfotyrosine.`
`CARBOHYD`	`43 43`		`O-linked (GalNAc...) [GlycoSuiteDB].`	`CAR_000143`
`DISULFID`	`6 14`		`By similarity.`
`DISULFID`	`16 28`		`By similarity.`
`DISULFID`	`22 37`		`By similarity.`

Sequence information

Length: 63 AA [This is the length of the unprocessed precursor]

Molecular weight: 6977 Da [This is the MW of the unprocessed precursor]

CRC64: 149A7369CC75A192 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MRYTACTESG QNQCICEGND VCGQGRNCQF DSSGKKCVEG EGTRKPQNEG QHDFDPIPEE 


YLS

P28512 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools