|
|
|
|
|
|
[1]
|
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=(68)-VIII;
DOI=10.1093/nar/20.17.4445; PubMed=1408746 [NCBI, ExPASy, EBI, Israel, Japan]
Kaufmann J.,
Klein A.;
"Gene dosage as a possible major determinant for equal expression levels of genes encoding RNA polymerase subunits in the hypotrichous ciliate Euplotes octocarinatus.";
Nucleic Acids Res. 20:4445-4450(1992).
|
[2]
|
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-124.
DOI=10.1093/nar/20.22.5985; PubMed=1461731 [NCBI, ExPASy, EBI, Israel, Japan]
Kaufmann J.,
Florian V.,
Klein A.;
"TGA cysteine codons and intron sequences in conserved and nonconserved positions are found in macronuclear RNA polymerase genes of Euplotes octocarinatus.";
Nucleic Acids Res. 20:5985-5989(1992).
|
|
|
|
- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing (By similarity).
- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
- SUBUNIT: Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits (By similarity).
- SUBCELLULAR LOCATION: Nucleus (By similarity).
- PTM: The tandem 7 residues repeats can be highly phosphorylated. The phosphorylation activates POL2.
- MISCELLANEOUS: The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion temporarily coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits. The ribonucleoside triphosphate is transferred by a rotation to the nucelotide addition (A) site for pairing with the template DNA. The catalytic A site involves three conserved aspartate residues of the RNA Pol II largest subunit which permanently coordinate a second magnesium ion.
- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
- SIMILARITY: Contains 1 C2H2-type zinc finger.
|
|
|
|
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| Length: 478 AA [This is the length of the partial sequence of the unprocessed precursor] |
Molecular weight: 54026 Da [This is the MW of the partial sequence of the unprocessed precursor] |
CRC64: 47A1ADD6ED98EB3D [This is a checksum on the sequence] |
|
10 20 30 40 50 60
MSRGTIYTES TMDFQKVRKI QFGLLDPKEI QAMSVVQVEN EKIYDNGIPT DGGINDLRMG
70 80 90 100 110 120
TMEKAMRCST CQGDSKECPG HFGHIELAQP VFHIGFIDLV KKILKCVCFN CNKLLITDKH
130 140 150 160 170 180
DKYSALKRVK DPKLKLNKVY KVCKDIKVCG KADRKSETYT EGSGQKQPRL RKTGLKIKAE
190 200 210 220 230 240
FPIDEDDPST NDNKRDLSAS ECLKILGRIS PDDCKFLGFD MVLARPEWLI ISRLPVAPPP
250 260 270 280 290 300
VRPSVCMGSN IRQEDDLTHQ YQQILKANNQ LRKHLSTANH IINENYQLLQ FYCATLIDNE
310 320 330 340 350 360
QAGQMVSRHK SGGKAIKAIR ARLKGKEGRL RGNLMGKRVD FSARTVITCD PTLDLDQLGV
370 380 390 400 410 420
PRSIAENITI PEVVTPQNID EMRKLVINGP NKWPGAKYIK GEGGKMIDLS YAKTTETFID
430 440 450 460 470
YGYVIERHLK NDDFVLFNRQ PSLHKMSIMG HRVKVLPYST FRLNLSVTAP YNADFDGS
|
P28364 in FASTA format |
|
ExPASy Home page
APAF Australia
