ID   RPA1_EUPOC              Reviewed;         638 AA.
AC   P28363;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   25-NOV-2008, entry version 50.
DE   RecName: Full=DNA-directed RNA polymerase I subunit RPA1;
DE            EC=2.7.7.6;
DE   AltName: Full=DNA-directed RNA polymerase I largest subunit;
DE   Flags: Fragment;
GN   Name=RPA1;
OS   Euplotes octocarinatus.
OC   Eukaryota; Alveolata; Ciliophora; Intramacronucleata; Spirotrichea;
OC   Hypotrichia; Euplotida; Euplotidae; Euplotes.
OX   NCBI_TaxID=5937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=(68)-VIII;
RX   MEDLINE=93027138; PubMed=1408746; DOI=10.1093/nar/20.17.4445;
RA   Kaufmann J., Klein A.;
RT   "Gene dosage as a possible major determinant for equal expression
RT   levels of genes encoding RNA polymerase subunits in the hypotrichous
RT   ciliate Euplotes octocarinatus.";
RL   Nucleic Acids Res. 20:4445-4450(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-94.
RX   MEDLINE=93096587; PubMed=1461731; DOI=10.1093/nar/20.22.5985;
RA   Kaufmann J., Florian V., Klein A.;
RT   "TGA cysteine codons and intron sequences in conserved and
RT   nonconserved positions are found in macronuclear RNA polymerase genes
RT   of Euplotes octocarinatus.";
RL   Nucleic Acids Res. 20:5985-5989(1992).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription
CC       of DNA into RNA using the four ribonucleoside triphosphates as
CC       substrates. RNA polymerase I is essentially used to transcribe
CC       ribosomal DNA units.
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- SUBUNIT: Each class of RNA polymerase is assembled from 9 to 14
CC       different polypeptides. This subunit is the largest component of
CC       RNA polymerase I.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- MISCELLANEOUS: Three distinct zinc-containing RNA polymerases are
CC       found in eukaryotic nuclei: polymerase I for the ribosomal RNA
CC       precursor, polymerase II for the mRNA precursor, and polymerase
CC       III for 5S and tRNA genes.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
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DR   EMBL; X66450; CAA47066.1; -; Genomic_DNA.
DR   PIR; S33884; S33884.
DR   HSSP; P04050; 1K83.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed RNA polymerase activity; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006350; P:transcription; IEA:InterPro.
DR   InterPro; IPR000722; RNA_pol_A.
DR   InterPro; IPR006592; RNA_pol_N.
DR   InterPro; IPR007080; RNA_pol_Rpb1_1.
DR   InterPro; IPR015699; RNA_polyI.
DR   Gene3D; G3DSA:2.40.40.30; RNA_pol_A; 1.
DR   Gene3D; G3DSA:3.90.1120.10; RNA_pol_Rpb1_1; 1.
DR   PANTHER; PTHR19376:SF11; RNA_polyI; 1.
DR   Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR   Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR   SMART; SM00663; RPOLA_N; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Metal-binding; Nucleotidyltransferase;
KW   Nucleus; Transcription; Transferase; Zinc; Zinc-finger.
FT   CHAIN         1   >638       DNA-directed RNA polymerase I subunit
FT                                RPA1.
FT                                /FTId=PRO_0000073927.
FT   ZN_FING      67     82       C2H2-type (Potential).
FT   NON_TER     638    638
SQ   SEQUENCE   638 AA;  73299 MW;  AB82F8BB9C22F5B5 CRC64;
     MATIFDSLIS SRLDGTRFSF YNDEEIQQMS VKEIHNPMAY DELNNPTSHG ICDESMGVSA
     LDKLSKCKTC GCDSVYCPGH MGHIKLTSTC YNPFTMKLLH SLLKSKCLVC HRLRISPKRI
     ELFEIRLKLI KLGYLVEAEK LSDFNHFSLE SIDSAIRILR KKTKGKTNKK EEKEDSEEVE
     EDKYAAKEAL ENLIQKEKEN REIFYSHISS ILQEDTPNES IGNAITVAIR DITKEIMNSV
     VPSKCPHCDV CNPRIKKDGA TKFFQMPLSN RDTKAMLSSH GRIDMRIDIS TMGAISEYDE
     EDDESSGESE VREGDEEQEK KQKYLSPIEV LEHMRRLWDV EDTLLNELFD NYKIFFMNNL
     LVSQNRFRPE SSGGKQSGDD RDYLHAHSAM LTKIINANIA FRRTIELKEN LTDDRNLETE
     SEKSKTQQID LSKTEITSKQ VIKAWAELQE SVNCYLDSSL AAKLENKEKP GLRQLLERKE
     GIFRMKMMGK RVNFAGRSVI SPDPYISTDQ IGVPEFIART ITFPEPAKNI EKLKRCVING
     AHKHPGANFI EYPNGERKAL ENMTLEEKKQ SSIIRKWRKI VYRHMQTGDP LLVNRQPTLH
     KPSIMAHNAI ILPKEQTIRM HYSNCKSYNA DFDGDEMK
//