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UniProtKB/Swiss-Prot entry P28343


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name THS1_VITVI
Primary accession number P28343
Secondary accession numbers A5AGH1 Q2HY09 Q54AD0
Integrated into Swiss-Prot on December 1, 1992
Sequence was last modified on October 1, 1996 (Sequence version 2)
Annotations were last modified on    September 2, 2008 (Entry version 61)
Name and origin of the protein
Protein name Stilbene synthase 1
Synonyms EC 2.3.1.95
Resveratrol synthase 1
Trihydroxystilbene synthase 1
StSy 1
Vitis stilbene synthase 1
PSV25
Gene name
Name: VINST1
Synonyms: STS2, VST1
ORFNames: GSVIVT00009226001, VITISV_035301
From
Vitis vinifera (Grape) [TaxID: 29760] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; Vitales; Vitaceae; Vitis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Optima;
DOI=10.1016/0014-5793(90)80961-H; PubMed=2200709 [NCBI, ExPASy, EBI, Israel, Japan]
Melchior F., Kindl H.;
"Grapevine stilbene synthase cDNA only slightly differing from chalcone synthase cDNA is expressed in Escherichia coli into a catalytically active enzyme.";
FEBS Lett. 268:17-20(1990).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Optima;
DOI=10.1016/0003-9861(91)90234-A; PubMed=1898048 [NCBI, ExPASy, EBI, Israel, Japan]
Melchior F., Kindl H.;
"Coordinate- and elicitor-dependent expression of stilbene synthase and phenylalanine ammonia-lyase genes in Vitis cv. Optima.";
Arch. Biochem. Biophys. 288:552-557(1991).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=cv. Optima;
Kobayashi S., Ding C., Nakamura Y., Nakajima I., Matsumoto R.;
"Kiwifruits (Actinidia deliciosa) transformed with a Vitis stilbene synthase gene produce piceid (resveratrol-glucoside).";
Plant Cell Rep. 19:904-910(2000).
[4]
NUCLEOTIDE SEQUENCE [MRNA].
Zhang Y., Yu O.;
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Pinot noir / PN40024;
DOI=10.1038/nature06148; PubMed=17721507 [NCBI, ExPASy, EBI, Israel, Japan]
Jaillon O., Aury J.-M., Noel B., Policriti A., Clepet C., Casagrande A., Choisne N., Aubourg S., Vitulo N., Jubin C., Vezzi A., Legeai F., Hugueney P., Dasilva C., Horner D., Mica E., Jublot D., Poulain J., Bruyere C., Billault A., Segurens B., Gouyvenoux M., Ugarte E., Cattonaro F., Anthouard V., Vico V., Del Fabbro C., Alaux M., Di Gaspero G., Dumas V., Felice N., Paillard S., Juman I., Moroldo M., Scalabrin S., Canaguier A., Le Clainche I., Malacrida G., Durand E., Pesole G., Laucou V., Chatelet P., Merdinoglu D., Delledonne M., Pezzotti M., Lecharny A., Scarpelli C., Artiguenave F., Pe M.E., Valle G., Morgante M., Caboche M., Adam-Blondon A.-F., Weissenbach J., Quetier F., Wincker P.;
"The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla.";
Nature 449:463-467(2007).
[6]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Pinot noir;
DOI=10.1371/journal.pone.0001326
Velasco R., Zharkikh A., Troggio M., Cartwright D.A., Cestaro A., Pruss D., Pindo M., FitzGerald L.M., Vezzulli S., Reid J., Malacarne G., Iliev D., Coppola G., Wardell B., Micheletti D., Macalma T., Facci M., Mitchell J.T., Perazzolli M., Eldredge G., Gatto P., Oyzerski R., Moretto M., Gutin N., Stefanini M., Chen Y., Segala C., Davenport C., Dematte L., Mraz A., Battilana J., Stormo K., Costa F., Tao Q., Si-Ammour A., Harkins T., Lackey A., Perbost C., Taillon B., Stella A., Solovyev V., Fawcett J.A., Sterck L., Vandepoele K., Grando S.M., Toppo S., Moser C., Lanchbury J., Bogden R., Skolnick M., Sgaramella V., Bhatnagar S.K., Fontana P., Gutin A., Van de Peer Y., Salamini F., Viola R.;
"A high quality draft consensus sequence of the genome of a heterozygous grapevine variety.";
PLoS ONE 2:E1326-E1326(2007).
[7]
INDUCTION BY OZONE AND PATHOGENS, AND TISSUE SPECIFICITY.
DOI=10.1023/A:1005830714852; PubMed=9225853 [NCBI, ExPASy, EBI, Israel, Japan]
Schubert R., Fischer R., Hain R., Schreier P.H., Bahnweg G., Ernst D., Sandermann H. Jr.;
"An ozone-responsive region of the grapevine resveratrol synthase promoter differs from the basal pathogen-responsive sequence.";
Plant Mol. Biol. 34:417-426(1997).
[8]
FUNCTION, BIOTECHNOLOGY, AND INDUCTION BY PHYTOPATHOGEN AND ABIOTIC STRESS.
DOI=10.1093/jexbot/52.358.901; PubMed=11432907 [NCBI, ExPASy, EBI, Israel, Japan]
Coutos-Thevenot P., Poinssot B., Bonomelli A., Yean H., Breda C., Buffard D., Esnault R., Hain R., Boulay M.;
"In vitro tolerance to Botrytis cinerea of grapevine 41B rootstock in transgenic plants expressing the stilbene synthase Vst1 gene under the control of a pathogen-inducible PR 10 promoter.";
J. Exp. Bot. 52:901-910(2001).
[9]
FUNCTION, AND BIOTECHNOLOGY.
Coutos-Thevenot P., Hain R., Schreier P.-H., Boulay M., Esnault R.;
"Nucleic acid comprising the sequence of a promoter unductible by stress and a gene sequence coding for a stilbene synthase.";
Patent number US6800794, 05-OCT-2004.
[10]
FUNCTION, AND BIOTECHNOLOGY.
DOI=10.1007/s00425-004-1343-1; PubMed=15309535 [NCBI, ExPASy, EBI, Israel, Japan]
Zhu Y.J., Agbayani R., Jackson M.C., Tang C.S., Moore P.H.;
"Expression of the grapevine stilbene synthase gene VST1 in papaya provides increased resistance against diseases caused by Phytophthora palmivora.";
Planta 220:241-250(2004).
[11]
BIOTECHNOLOGY.
DOI=10.1023/B:TRAG.0000034658.64990.7f; PubMed=15359598 [NCBI, ExPASy, EBI, Israel, Japan]
Giorcelli A., Sparvoli F., Mattivi F., Tava A., Balestrazzi A., Vrhovsek U., Calligari P., Bollini R., Confalonieri M.;
"Expression of the stilbene synthase (StSy) gene from grapevine in transgenic white poplar results in high accumulation of the antioxidant resveratrol glucosides.";
Transgenic Res. 13:203-214(2004).
[12]
IDENTIFICATION BY MASS SPECTROMETRY, AND INDUCTION BY JA.
DOI=10.1111/j.1469-8137.2005.01383.x; PubMed=15869650 [NCBI, ExPASy, EBI, Israel, Japan]
Tassoni A., Fornale S., Franceschetti M., Musiani F., Michael A.J., Perry B., Bagni N.;
"Jasmonates and Na-orthovanadate promote resveratrol production in Vitis vinifera cv. Barbera cell cultures.";
New Phytol. 166:895-905(2005).
[13]
BIOTECHNOLOGY.
DOI=10.1111/j.1467-7652.2004.00099.x; PubMed=17168899 [NCBI, ExPASy, EBI, Israel, Japan]
Giovinazzo G., D'Amico L., Paradiso A., Bollini R., Sparvoli F., DeGara L.;
"Antioxidant metabolite profiles in tomato fruit constitutively expressing the grapevine stilbene synthase gene.";
Plant Biotechnol. J. 3:57-69(2005).
[14]
INDUCTION BY JA.
DOI=10.1021/jf0618022; PubMed=17117799 [NCBI, ExPASy, EBI, Israel, Japan]
Belhadj A., Saigne C., Telef N., Cluzet S., Bouscaut J., Corio-Costet M.-F., Merillon J.-M.;
"Methyl jasmonate induces defense responses in grapevine and triggers protection against Erysiphe necator.";
J. Agric. Food Chem. 54:9119-9125(2006).
[15]
INDUCTION BY UV LIGHT.
DOI=10.1007/s00299-006-0172-z; PubMed=16802117 [NCBI, ExPASy, EBI, Israel, Japan]
Richter A., de Kathen A., de Lorenzo G., Briviba K., Hain R., Ramsay G., Jacobsen H.-J., Kiesecker H.;
"Transgenic peas (Pisum sativum) expressing polygalacturonase inhibiting protein from raspberry (Rubus idaeus) and stilbene synthase from grape (Vitis vinifera).";
Plant Cell Rep. 25:1166-1173(2006).
[16]
BIOTECHNOLOGY.
DOI=10.1128/AEM.00609-06; PubMed=16885328 [NCBI, ExPASy, EBI, Israel, Japan]
Beekwilder J., Wolswinkel R., Jonker H., Hall R., de Vos C.H.R., Bovy A.;
"Production of resveratrol in recombinant microorganisms.";
Appl. Environ. Microbiol. 72:5670-5672(2006).
[17]
INDUCTION BY ERGOSTEROL.
DOI=10.1094/MPMI-19-1103; PubMed=17022174 [NCBI, ExPASy, EBI, Israel, Japan]
Laquitaine L., Gomes E., Francois J., Marchive C., Pascal S., Hamdi S., Atanassova R., Delrot S., Coutos-Thevenot P.;
"Molecular basis of ergosterol-induced protection of grape against botrytis cinerea: induction of type I LTP promoter activity, WRKY, and stilbene synthase gene expression.";
Mol. Plant Microbe Interact. 19:1103-1112(2006).
[18]
REVIEW.
DOI=10.2741/2432; PubMed=17569614 [NCBI, ExPASy, EBI, Israel, Japan]
Shankar S., Singh G., Srivastava R.K.;
"Chemoprevention by resveratrol: molecular mechanisms and therapeutic potential.";
Front. Biosci. 12:4839-4854(2007).
Comments
  • FUNCTION: Mediates resistance to pathogens which are sensitive to stilbenes such as Botrytis cinerea, Eutypa lata and Plasmopora viticola by enhancing the production of phytoalexins. Confers resistance to Phytophthora palmivora when expressed in papaya.
  • CATALYTIC ACTIVITY: 3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + 3,4',5-trihydroxy-stilbene + 4 CO2.
  • PATHWAY: Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from 4-coumaric acid: step 2/2.
  • SUBUNIT: Homodimer (By similarity).
  • SUBCELLULAR LOCATION: Cytoplasm.
  • TISSUE SPECIFICITY: In leaves, expressed in palisade and spongy parenchyma cells and, to a lesser extent, in epidermal cells after induction.
  • INDUCTION: By stress, including UV light, ozone and pathogens such as B.cinerea. Strongly induced by ergosterol, a non-specific fungal elicitor. Positively regulated by jasmonate (JA) and methyl-jasmonate (MeJA). Induction by phytopathogen attack decreases with grape berry ripening.
  • BIOTECHNOLOGY: Driven by a stress-inducible promoter, stilbene synthase is used to improve resistance to pathogens which are sensitive to stilbenes such as B.cinerea, P.palmivora, E.lata or P.viticola.
  • BIOTECHNOLOGY: Expression in heterologous systems (e.g. S.cerevisiae and Populus alba) is used to produce resveratrol for human food and medication. Resveratrol, a product of the chemical reaction catalized by stilbene synthase, exhibits several clinical benefits for human diseases, such as cancers, cardiovascular diseases and has positive effects on longevity.
  • SIMILARITY: Belongs to the chalcone/stilbene synthases family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
S63225; -; NOT_ANNOTATED_CDS; mRNA.[EMBL / GenBank / DDBJ]
S63227; -; NOT_ANNOTATED_CDS; mRNA.[EMBL / GenBank / DDBJ]
AB046375; BAB20980.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
DQ366302; ABC84860.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CU459442; CAO15528.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AM426171; CAN60215.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S11044; S11044.
3D structure databases
HSSP P30074; 1CGZ. [HSSP ENTRY / PDB]
SMR P28343; 3-387.
ModBase P28343.
Family and domain databases
InterPro IPR012328; Chal_sti_synt_C.
IPR001099; Chal_sti_synt_N.
IPR011141; Polyketide_synthase_type-III.
IPR016038; Thiolase-like_subgr.
Graphical view of domain structure.
Gene3D G3DSA:3.40.47.10; Thiolase-like_subgr; 2.
Pfam PF02797; Chal_sti_synt_C; 1.
PF00195; Chal_sti_synt_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000451; PKS_III; 1.
ProDom PD000453; N-C_synthase; 1.
[Domain structure / List of seq. sharing at least 1 domain]
PROSITE PS00441; CHALCONE_SYNTH; 1.
BLOCKS P28343.
Other
ProtoNet P28343.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Acyltransferase; Cytoplasm; Genetically modified food; Plant defense; Stress response; Transferase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   392  392     Stilbene synthase 1. PRO_0000216082
REGION   55    58  4     Substrate binding (By similarity). 
REGION   305   307  3     Substrate binding (By similarity). 
ACT_SITE   164   164        By similarity. 
BINDING   267   267        Substrate; via carbonyl oxygen (By similarity). 
CONFLICT   387   389        VPT -> IPM (in Ref. 1; S63227 and 4; ABC84860). 
Sequence information
Length: 392 AA [This is the length of the unprocessed precursor] Molecular weight: 42840 Da [This is the MW of the unprocessed precursor] CRC64: 0C2E8C2553792431 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MASVEEFRNA QRAKGPATIL AIGTATPDHC VYQSDYADYY FRVTKSEHMT ELKKKFNRIC 

        70         80         90        100        110        120 
DKSMIKKRYI HLTEEMLEEH PNIGAYMAPS LNIRQEIITA EVPRLGRDAA LKALKEWGQP 

       130        140        150        160        170        180 
KSKITHLVFC TTSGVEMPGA DYKLANLLGL ETSVRRVMLY HQGCYAGGTV LRTAKDLAEN 

       190        200        210        220        230        240 
NAGARVLVVC SEITVVTFRG PSEDALDSLV GQALFGDGSS AVIVGSDPDV SIERPLFQLV 

       250        260        270        280        290        300 
SAAQTFIPNS AGAIAGNLRE VGLTFHLWPN VPTLISENIE KCLTQAFDPL GISDWNSLFW 

       310        320        330        340        350        360 
IAHPGGPAIL DAVEAKLNLE KKKLEATRHV LSEYGNMSSA CVLFILDEMR KKSLKGEKAT 

       370        380        390 
TGEGLDWGVL FGFGPGLTIE TVVLHSVPTV TN 

P28343 in FASTA format

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