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UniProtKB/Swiss-Prot entry P28337

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GCST_CHICK
Primary accession number P28337
Secondary accession numbers None
Integrated into Swiss-Prot on December 1, 1992
Sequence was last modified on October 1, 1993 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 54)
Name and origin of the protein
Protein name Aminomethyltransferase, mitochondrial [Precursor]
Synonyms EC 2.1.2.10
Glycine cleavage system T protein
GCVT
Gene name
Name: AMT
From
Gallus gallus (Chicken) [TaxID: 9031] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Archosauria; Dinosauria; Saurischia; Theropoda; Coelurosauria; Aves; Neognathae; Galliformes; Phasianidae; Phasianinae; Gallus.
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
PubMed=1526969 [NCBI, ExPASy, EBI, Israel, Japan]
Okamura-Ikeda K., Fujiwara K., Motokawa Y.;
"Molecular cloning of a cDNA encoding chicken T-protein of the glycine cleavage system and expression of the functional protein in Escherichia coli. Effect of mRNA secondary structure in the translational initiation region on expression.";
J. Biol. Chem. 267:18284-18290(1992).
[2]
 NUCLEOTIDE SEQUENCE [MRNA] OF 179-392.
TISSUE=Liver;
PubMed=2002038 [NCBI, ExPASy, EBI, Israel, Japan]
Okamura-Ikeda K., Fujiwara K., Yamamoto M., Hiraga K., Motokawa Y.;
"Isolation and sequence determination of cDNA encoding T-protein of the glycine cleavage system.";
J. Biol. Chem. 266:4917-4921(1991).
Comments
  • FUNCTION: The glycine cleavage system catalyzes the degradation of glycine.
  • CATALYTIC ACTIVITY: [Protein]-S8-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3.
  • SUBUNIT: The glycine cleavage system is composed of four proteins: P, T, L and H.
  • SUBCELLULAR LOCATION: Mitochondrion.
  • SIMILARITY: Belongs to the gcvT family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
D11162; BAA01937.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A44167; A44167.
RefSeq NP_990119.1; -.
UniGene Gga.1002
3D structure databases
ModBase P28337.
Enzyme and pathway databases
BioCyc MetaCyc:MON-12927; -.
Ontologies
GO
GO:0005739; Cellular component: mitochondrion (inferred from electronic annotation from UniProtKB-KW).
GO:0004047; Molecular function: aminomethyltransferase activity (inferred from electronic annotation from InterPro).
GO:0008483; Molecular function: transaminase activity (inferred from electronic annotation from UniProtKB-KW).
GO:0006546; Biological process: glycine catabolic process (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR013977; GCV_T_C.
IPR006222; GCV_T_N.
IPR006223; GcvT.
Graphical view of domain structure.
Pfam PF01571; GCV_T; 1.
PF08669; GCV_T_C; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF006487; GcvT; 1.
TIGRFAMs TIGR00528; gcvT; 1.
BLOCKS P28337.
ProtoNet P28337.
Genome annotation databases
GeneID 395566; -.
KEGG gga:395566; -.
Phylogenomic databases
HOVERGEN P28337; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Aminotransferase; Mitochondrion; Transferase; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom  To Length Description FTId
TRANSIT   1    16  16     Mitochondrion. 
CHAIN   17   392  376     Aminomethyltransferase, mitochondrial. PRO_0000010757
Sequence information
Length: 392 AA [This is the length of the unprocessed precursor] Molecular weight: 42058 Da [This is the MW of the unprocessed precursor] CRC64: A022434038AF71E0 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MLRAGCRAAL ARRHLSSAPE GLKQTPLDAL HRARGGRMVP FAGWSLPVQY GRGHLESHLH 

        70         80         90        100        110        120 
TRRHCSLFDV SHMLQTRVYG RDRVRFLESL VVGDIAELRP GQGTLTLLTN ERGDIVDDLI 

       130        140        150        160        170        180 
VTNTAEDHLY VVSNAGCADK DRAVMEGRAA ELRAAGGDVH LEVSGQRAAG VQGPSMAQVL 

       190        200        210        220        230        240 
QAGLPDDLTK LTFMTSTATT VFGVPGCRVT RCGYTGEDGV EISVPAGRAV ELAERLLGCP 

       250        260        270        280        290        300 
EVWPAGLAAR DSLRLEAGLC LYGNDIDEST TPVEAGLLWT LGKRRRTAMD FPGAAIIMEQ 

       310        320        330        340        350        360 
VKEKPKRKRV GLTSVGPPLR PPAAILGPEG TPVGTVTSGC PSPSLGKNIA MGYVQAAHSR 

       370        380        390 
PGTTLTVEVR KKQHPALVTK MPFVPTHYYM AK
P28337 in FASTA format

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