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UniProtKB/Swiss-Prot entry P28296


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ORYZ_ASPFU
Primary accession number P28296
Secondary accession number Q4WQ71
Integrated into Swiss-Prot on December 1, 1992
Sequence was last modified on December 6, 2005 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 64)
Name and origin of the protein
Protein name Oryzin [Precursor]
Synonyms EC 3.4.21.63
Alkaline proteinase
ALP
Elastase
Elastinolytic serine proteinase
Gene name
Name: alk1
Synonyms: alp
ORFNames: AFUA_4G11800
From
Aspergillus fumigatus (Sartorya fumigata) [TaxID: 5085] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Trichocomaceae; mitosporic Trichocomaceae; Aspergillus.
Protein existence 3: Inferred from homology;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0378-1097(92)90506-J; PubMed=1601287 [NCBI, ExPASy, EBI, Israel, Japan]
Jaton-Ogay K., Suter M., Crameri R., Falchetto R., Fatih A., Monod M.;
"Nucleotide sequence of a genomic and a cDNA clone encoding an extracellular alkaline protease of Aspergillus fumigatus.";
FEMS Microbiol. Lett. 71:163-168(1992).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=8500876 [NCBI, ExPASy, EBI, Israel, Japan]
Kolattukudy P.E., Lee J.D., Rogers L.M., Zimmerman P., Ceselski S., Fox B., Stein B., Copelan E.A.;
"Evidence for possible involvement of an elastolytic serine protease in aspergillosis.";
Infect. Immun. 61:2357-2368(1993).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Af293 / CBS 101355 / FGSC A1100;
DOI=10.1038/nature04332; PubMed=16372009 [NCBI, ExPASy, EBI, Israel, Japan]
Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L., Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W.;
"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus.";
Nature 438:1151-1156(2005).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z11580; CAA77666.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M99420; AAB07672.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AAHF01000005; EAL89613.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S22184; S22184.
RefSeq XP_751651.1; -.
3D structure databases
HSSP P06873; 1IC6. [HSSP ENTRY / SWISS-3DIMAGE / PDB]
ModBase P28296.
Protein family/group databases
MEROPS S08.053; -.
Ontologies
GO
GO:0005576; Cellular component: extracellular region (inferred from electronic annotation from UniProtKB-KW).
GO:0042802; Molecular function: identical protein binding (inferred from electronic annotation from InterPro).
GO:0004252; Molecular function: serine-type endopeptidase activity (inferred from electronic annotation from InterPro).
GO:0043086; Biological process: negative regulation of catalytic activity (inferred from electronic annotation from InterPro).
GO:0009405; Biological process: pathogenesis (inferred from electronic annotation from UniProtKB-KW).
GO:0006508; Biological process: proteolysis (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR000209; Pept_S8_S53.
IPR015500; Peptidase_S8_subtilisin-rel.
IPR010259; Prot_inh_S8A.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.200; Pept_S8_S53; 1.
PANTHER PTHR10795; SubtilSerProt; 1.
Pfam PF00082; Peptidase_S8; 1.
PF05922; Subtilisin_N; 1.
Pfam graphical view of domain structure.
PRINTS PR00723; SUBTILISIN.
PROSITE PS00136; SUBTILASE_ASP; 1.
PS00137; SUBTILASE_HIS; 1.
PS00138; SUBTILASE_SER; 1.
BLOCKS P28296.
ProtoNet P28296.
Genome annotation databases
GeneID 3509271; -.
KEGG afm:AFUA_4G11800; -.
Phylogenomic databases
HOGENOM P28296; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Glycoprotein; Hydrolase; Protease; Secreted; Serine protease; Signal; Virulence; Zymogen.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    21  21     By similarity. 
PROPEP   22   121  100     By similarity. PRO_0000027082
CHAIN   122   403  282     Oryzin. PRO_0000027083
ACT_SITE   162   162        Charge relay system (By similarity). 
ACT_SITE   193   193        Charge relay system (By similarity). 
ACT_SITE   349   349        Charge relay system (By similarity). 
CARBOHYD   253   253        N-linked (GlcNAc...) (Potential). 
CARBOHYD   307   307        N-linked (GlcNAc...) (Potential). 
CARBOHYD   367   367        N-linked (GlcNAc...) (Potential). 
CONFLICT   106   107        SA -> RG (in Ref. 1; CAA77666). 
CONFLICT   295   295        A -> R (in Ref. 2; AAB07672). 
Sequence information
Length: 403 AA [This is the length of the unprocessed precursor] Molecular weight: 42190 Da [This is the MW of the unprocessed precursor] CRC64: 2D900D1AE22CDD4A [This is a checksum on the sequence]
        10         20         30         40         50         60 
MLSIKRTLLL LGAVLPAVFG APVQETRRAA QKIPGKYIVT FKPGTDTATI ESHTLWATDL 

        70         80         90        100        110        120 
HKRNLERRDT TSGEPPVGIE KSYKIKDFAA YAGSFDDATI EEIRKSADVA HVEEDQIWYL 

       130        140        150        160        170        180 
DALTTQKGAP WGLGSISHKG QASTDYIYDT SAGAGTYAYV VDSGINVNHV EFESRASLAY 

       190        200        210        220        230        240 
NAAGGSHVDS IGHGTHVAGT IGGKTYGVAK KTNLLSVKVF QGESSSTSII LDGFNWAVND 

       250        260        270        280        290        300 
IVSKGRTKKA AINMSLGGGY SYAFNNAVEN AFDEGVLSVV AAGNENSDAS NTSPASAPNA 

       310        320        330        340        350        360 
LTVAAINKSN ARASFSNYGS VVDIFAPGQD ILSAWIGSTT ATNTISGTSM ATPHIVGLSV 

       370        380        390        400 
YLMGLENLSG PAAVTARIKE LATNGVVTNV KGSPNKLAYN GNA 

P28296 in FASTA format

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