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UniProtKB/Swiss-Prot entry P28227

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MAOX_ANAPL
Primary accession number P28227
Secondary accession numbers None
Integrated into Swiss-Prot on December 1, 1992
Sequence was last modified on December 1, 1992 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 51)
Name and origin of the protein
Protein name NADP-dependent malic enzyme
Synonyms NADP-ME
EC 1.1.1.40
Gene name
Name: ME1
From
Anas platyrhynchos (Domestic duck) [TaxID: 8839] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Archosauria; Dinosauria; Saurischia; Theropoda; Coelurosauria; Aves; Neognathae; Anseriformes; Anatidae; Anas.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, AND COFACTOR.
TISSUE=Liver;
PubMed=1622402 [NCBI, ExPASy, EBI, Israel, Japan]
Hsu R.Y., Glynias M.J., Satterlee J.D., Feeney R., Clarke A.R., Emery D.S., Roe B.A., Wilson R.K., Goodridge A.G., Holbrook J.J.;
"Duck liver 'malic' enzyme. Expression in Escherichia coli and characterization of the wild-type enzyme and site-directed mutants.";
Biochem. J. 284:869-876(1992).
[2]
 PROTEIN SEQUENCE OF 86-107.
TISSUE=Liver;
DOI=10.1016/0167-4838(91)90065-8; PubMed=1911848 [NCBI, ExPASy, EBI, Israel, Japan]
Satterlee J.D., Hsu R.Y.;
"Duck liver malic enzyme: sequence of a tryptic peptide containing the cysteine residue labeled by the substrate analog bromopyruvate.";
Biochim. Biophys. Acta 1079:247-252(1991).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X66418; CAA47049.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S23435; S23435.
3D structure databases
HSSP P40927; 1GQ2. [HSSP ENTRY / PDB]
ModBase P28227.
Family and domain databases
InterPro IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR001891; Malic_OxRdtase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF00390; malic; 1.
PF03949; Malic_M; 1.
Pfam graphical view of domain structure.
PRINTS PR00072; MALOXRDTASE.
PROSITE PS00331; MALIC_ENZYMES; 1.
BLOCKS P28227.
Phylogenomic databases
HOVERGEN P28227; -.
Other
ProtoNet P28227.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cytoplasm; Direct protein sequencing; Metal-binding; NADP; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   557  557     NADP-dependent malic enzyme. PRO_0000160196
NP_BIND   290   307  18     NADP (By similarity). 
ACT_SITE   91    91        Proton donor (By similarity). 
ACT_SITE   162   162        Proton acceptor (By similarity). 
METAL   234   234        Divalent metal cation (By similarity). 
METAL   235   235        Divalent metal cation (By similarity). 
METAL   258   258        Divalent metal cation (By similarity). 
BINDING   144   144        NADP (By similarity). 
BINDING   258   258        NADP (By similarity). 
BINDING   397   397        NADP (By similarity). 
SITE   258   258  1     Important for activity (By similarity). 
Sequence information
Length: 557 AA [This is the length of the unprocessed precursor] Molecular weight: 61898 Da [This is the MW of the unprocessed precursor] CRC64: BE6ED42C7017EF19 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MKRGYEVLRD PHLNKGMAFT LEERQQLNIH GLLPPCFLGQ DVQVFSILKN FERLTSDLDR 

        70         80         90        100        110        120 
YILLMSLQDR NEKLFYKVLT SDIERFMPIV YTPTVGLACQ QYGLAFRRPR GLFITIHDRG 

       130        140        150        160        170        180 
HIATMLKSWP ESVIKAIVVT DGERILGLGD LGCYGMGIPV GKLALYTACG GVKPHECLPV 

       190        200        210        220        230        240 
MLDVGTDNEA LLKDPLYIGL RHKRIRGQAY DDLLDEFMEA VTSRYGMNCL IQFEDFANAN 

       250        260        270        280        290        300 
AFRLLHKYRN KYCTFNDDIQ GTASVAVAGL LAALRITKNR LSDHTVLFQG AGEAALGIAN 

       310        320        330        340        350        360 
LIVMAMEKEG VSKEAAVKRI WMVDSKGLIV KGRASLTAEK TRFAHEHAEM KNLEDIVKDI 

       370        380        390        400        410        420 
KPSVLIGVAA IGGAFTKEIL QDMAAFNKRP IIFALSNPTS KAECTAEQCY KYTEGRGIFA 

       430        440        450        460        470        480 
SGSPFDPVTL PNGKTLYPGQ GNNSYVFPGV ALGVIACGLK HIGEDVFLTT AEVIAEQVSE 

       490        500        510        520        530        540 
ENLQEGRLYP PLVTIQHVSL KIAVRIAEEA YRNNTASTYP QPKDLEAFIQ SQIYSTDYNS 

       550 
FVADSYTWPE EAMKVKL
P28227 in FASTA format

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