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[1]
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NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Iroquois;
DOI=10.1007/BF00029858; PubMed=8193301 [NCBI, ExPASy, EBI, Israel, Japan]
McKhann H.I.,
Hirsch A.M.;
"Isolation of chalcone synthase and chalcone isomerase cDNAs from alfalfa (Medicago sativa L.): highest transcript levels occur in young roots and root tips.";
Plant Mol. Biol. 24:767-777(1994).
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[2]
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ERRATUM.
McKhann H.I.,
Hirsch A.M.;
Plant Mol. Biol. 25:759-759(1994).
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[3]
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X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH (2S)-NARINGENIN, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND MUTAGENESIS OF TYR-106.
DOI=10.1038/79025; PubMed=10966651 [NCBI, ExPASy, EBI, Israel, Japan]
Jez J.M.,
Bowman M.E.,
Dixon R.A.,
Noel J.P.;
"Structure and mechanism of the evolutionarily unique plant enzyme chalcone isomerase.";
Nat. Struct. Biol. 7:786-791(2000).
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[4]
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X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND MUTAGENESIS OF THR-48; TYR-106; ASN-113 AND THR-190.
DOI=10.1021/bi0255266; PubMed=11955065 [NCBI, ExPASy, EBI, Israel, Japan]
Jez J.M.,
Bowman M.E.,
Noel J.P.;
"Role of hydrogen bonds in the reaction mechanism of chalcone isomerase.";
Biochemistry 41:5168-5176(2002).
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[5]
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X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
DOI=10.1074/jbc.M109224200; PubMed=11698411 [NCBI, ExPASy, EBI, Israel, Japan]
Jez J.M.,
Noel J.P.;
"Reaction mechanism of chalcone isomerase. pH dependence, diffusion control, and product binding differences.";
J. Biol. Chem. 277:1361-1369(2002).
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- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic chalcones into tricyclic (S)-flavanones. Responsible for the isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone) into naringenin.
- CATALYTIC ACTIVITY: A chalcone = a flavanone.
- BIOPHYSICOCHEMICAL PROPERTIES:
| Kinetic parameters: |
KM=8.4 µM for 4,2',4'-trihydroxychalcone (at pH 7.5 and 25 degrees Celsius); | | KM=15.7 µM for 6'-deoxychalcone (at pH 7.5 and 25 degrees Celsius); | | KM=22.7 µM for 2',4'-dihydroxychalcone (at pH 7.5 and 25 degrees Celsius); | | KM=42.5 µM for 4,2'-dihydroxychalcone (at pH 7.5 and 25 degrees Celsius); | | KM=112 µM for 4,2',4',6'-tetrahydroxychalcone (at pH 7.5 and 25 degrees Celsius); | | pH dependence: |
Optimum pH is 7-8.5 with 4,2',4'-trihydroxychalcone as substrate, at 25 degrees Celsius; | |
- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
- DEVELOPMENTAL STAGE: Highest expression in young root tips.
- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of flavonoids, a large class of secondary plant metabolites, many of which are brightly colored.
- SIMILARITY: Belongs to the chalcone isomerase family.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 222 AA [This is the length of the unprocessed precursor] |
Molecular weight: 23826 Da [This is the MW of the unprocessed precursor] |
CRC64: 7767A72084AB4800 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MAASITAITV ENLEYPAVVT SPVTGKSYFL GGAGERGLTI EGNFIKFTAI GVYLEDIAVA
70 80 90 100 110 120
SLAAKWKGKS SEELLETLDF YRDIISGPFE KLIRGSKIRE LSGPEYSRKV MENCVAHLKS
130 140 150 160 170 180
VGTYGDAEAE AMQKFAEAFK PVNFPPGASV FYRQSPDGIL GLSFSPDTSI PEKEAALIEN
190 200 210 220
KAVSSAVLET MIGEHAVSPD LKRCLAARLP ALLNEGAFKI GN
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P28012 in FASTA format |
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