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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=1551396 [NCBI, ExPASy, EBI, Israel, Japan]
Fischer R.,
Hengstenberg W.;
"Mannitol-specific enzyme II of the phosphoenolpyruvate-dependent phosphotransferase system of Staphylococcus carnosus. Sequence and expression in Escherichia coli and structural comparison with the enzyme IImannitol of Escherichia coli.";
Eur. J. Biochem. 204:963-969(1992).
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[2]
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SEQUENCE REVISION.
Hengstenberg W.;
Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
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[3]
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ACTIVE SITE CYS-432.
PubMed=7588734 [NCBI, ExPASy, EBI, Israel, Japan]
Pogge von Strandmann R.,
Weigt C.,
Fischer R.,
Meyer H.E.,
Kalbitzer H.R.,
Hengstenberg W.;
"Expression, purification and characterization of the enzyme II mannitol-specific domain from Staphylococcus carnosus and determination of the active-site cysteine residue.";
Eur. J. Biochem. 233:116-122(1995).
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- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in mannitol transport.
- CATALYTIC ACTIVITY: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
- SUBUNIT: Homodimer.
- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (Potential).
- INDUCTION: By mannitol.
- DOMAIN: The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
- DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
- SIMILARITY: Contains 1 PTS EIIB type-2 domain.
- SIMILARITY: Contains 1 PTS EIIC type-2 domain.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 518 AA [This is the length of the unprocessed precursor] |
Molecular weight: 55686 Da [This is the MW of the unprocessed precursor] |
CRC64: A0DE0B9E4BA74FA5 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MDTMSNSQQN KGIGRKVQAF GSFLSSMIMP NIGAFIAWGF IAAIFIDNGW FPNKDLAQLA
70 80 90 100 110 120
GPMITYLIPL LIAFSGGRLI HDLRGGIIAA TATMGVIVAL PDTPMLLGAM IMGPLVGWLM
130 140 150 160 170 180
KKTDEFVQPR TPQGFEMLFN NFSAGILGFI MTIFGFEVLA PIMKFIMHIL SVGVEALVHA
190 200 210 220 230 240
HLLPLVSILV EPAKIVFLNN AINHGVFTPL GADQAAHAGQ SILYTIESNP GPGIGVLIAY
250 260 270 280 290 300
MIFGKGTAKA TSYGAGIIQF FGGIHEIYFP YVLMRPLLFV SVILGGMTGV ATYSLLDFGF
310 320 330 340 350 360
KTPASPGSII VYAINAPKGE FLHMLTGVVL AALVSFVVSA LILKFTKDPK QDLAEATAQM
370 380 390 400 410 420
EATKGKKSSV ASKLSAKDDN KAADNKTAET TTATAASNKA EDKDSDELLD DYNTEDVDAH
430 440 450 460 470 480
NYNNVDHVIF ACDAGMGSSA MGASMLRNKF KNAGLENIQV TNTAINQLPK NAQLVITQKK
490 500 510
LTDRAIKQSP DAIHISVENF LNSPRYEELI NNLKEDQD
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P28008 in FASTA format |
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