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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=HR;
PubMed=1907281 [NCBI, ExPASy, EBI, Israel, Japan]
Gibson J.L.,
Falcone D.L.,
Tabita F.R.;
"Nucleotide sequence, transcriptional analysis, and expression of genes encoded within the form I CO2 fixation operon of Rhodobacter sphaeroides.";
J. Biol. Chem. 266:14646-14653(1991).
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[2]
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KINETIC CHARACTERIZATION, AND MUTAGENESIS.
STRAIN=FI;
DOI=10.1006/abbi.1998.0979; PubMed=9882445 [NCBI, ExPASy, EBI, Israel, Japan]
Horken K.M.,
Tabita F.R.;
"Closely related form I ribulose bisphosphate carboxylase/oxygenase molecules that possess different CO2/O2 substrate specificities.";
Arch. Biochem. Biophys. 361:183-194(1999).
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- FUNCTION: RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site (By similarity).
- CATALYTIC ACTIVITY: 2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.
- CATALYTIC ACTIVITY: 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.
- COFACTOR: Binds 1 magnesium ion per subunit (By similarity).
- BIOPHYSICOCHEMICAL PROPERTIES:
| Kinetic parameters: |
KM=50 µM for ribulose 1,5-bisphosphate; | | KM=22 µM for CO2; | | Vmax=2.5 µmol/min/mg enzyme with CO2 as substrate; | | Note=The CO(2)/O(2) specificity factor (tau) is 56; | |
- SUBUNIT: Heterohexadecamer of 8 large chains and 8 small chains (By similarity).
- INDUCTION: Expression of this operon predominates when carbon dioxide is limiting.
- MISCELLANEOUS: The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" (By similarity).
- SIMILARITY: Belongs to the RuBisCO large chain family. Type I subfamily.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 486 AA [This is the length of the unprocessed precursor] |
Molecular weight: 53686 Da [This is the MW of the unprocessed precursor] |
CRC64: 82B91D700303C3C0 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MDTKTTEIKG KERYKAGVLK YAQMGYWDGD YVPKDTDVLA LFRITPQEGV DPVEAAAAVA
70 80 90 100 110 120
GESSTATWTV VWTDRLTACD SYRAKAYRVE PVPGTPGQYF CYVAYDLILF EEGSIANLTA
130 140 150 160 170 180
SIIGNVFSFK PLKAARLEDM RFPVAYVKTY KGPPTGIVGE RERLDKFGKP LLGATTKPKL
190 200 210 220 230 240
GLSGKNYGRV VYEGLKGGLD FMKDDENINS QPFMHWRDRF LYVMEAVNLA SAQTGEVKGH
250 260 270 280 290 300
YLNITAGTME EMYRRAEFAK SLGSVIVMVD LIIGYTAIQS ISEWCRQNDM ILHMHRAGHG
310 320 330 340 350 360
TYTRQKNHGI SFRVIAKWLR LAGVDHLHCG TAVGKLEGDP LTVQGYYNVC REPFNTVDLP
370 380 390 400 410 420
RGIFFEQDWA DLRKVMPVAS GGIHAGQMHQ LLSLFGDDVV LQFGGGTIGH PMGIQAGATA
430 440 450 460 470 480
NRVALEAMVL ARNEGRNIDV EGPEILRAAA KWCKPLEAAL DTWGNITFNY TSTDTSDFVP
TASVAM
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P27997 in FASTA format |
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