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UniProtKB/Swiss-Prot entry P27969

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NIA2_HORVU
Primary accession number P27969
Secondary accession numbers None
Integrated into Swiss-Prot on August 1, 1992
Sequence was last modified on August 1, 1992 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 63)
Name and origin of the protein
Protein name Nitrate reductase [NADH] [Fragment]
Synonyms NR
EC 1.7.1.1
Gene name None
From
Hordeum vulgare (Barley) [TaxID: 4513] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Pooideae; Triticeae; Hordeum.
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Steptoe;
TISSUE=Leaf;
DOI=10.1007/BF00273931; PubMed=1865878 [NCBI, ExPASy, EBI, Israel, Japan]
Schnorr K.M., Juricek M., Huang C., Culley D., Kleinhofs A.;
"Analysis of barley nitrate reductase cDNA and genomic clones.";
Mol. Gen. Genet. 227:411-416(1991).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X57844; CAA40975.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S17454; RDBHNS.
3D structure databases
HSSP P17571; 2CND. [HSSP ENTRY / PDB]
SMR P27969; 653-912.
ModBase P27969.
Organism-specific databases
Gramene P27969; -.
Ontologies
GO
GO:0009055; Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0050660; Molecular function: FAD binding (inferred from electronic annotation from InterPro).
GO:0020037; Molecular function: heme binding (inferred from electronic annotation from InterPro).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from InterPro).
GO:0030151; Molecular function: molybdenum ion binding (inferred from electronic annotation from InterPro).
GO:0009703; Molecular function: nitrate reductase (NADH) activity (inferred from electronic annotation from EC).
GO:0042128; Biological process: nitrate assimilation (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001199; Cyt_B5.
IPR001834; Cyt_B5_reductase.
IPR001709; FPN_cyt_redctse.
IPR005066; MoCF_OxRdtse_dimer.
IPR008335; Mopterin_OxRdtase_euk.
IPR012137; Nitr_rd_NADH.
IPR008333; OxRdtase_FAD-bd.
IPR001433; OxRdtase_FAD/NAD_bd.
IPR000572; OxRdtase_Mopterin-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.10.120.10; Cyt_B5; 1.
G3DSA:2.60.40.650; MoCF_oxrdtse_dimer; 1.
G3DSA:3.90.420.10; Oxred_molyb_bd; 1.
Pfam PF00173; Cyt-b5; 1.
PF00970; FAD_binding_6; 1.
PF03404; Mo-co_dimer; 1.
PF00175; NAD_binding_1; 1.
PF00174; Oxidored_molyb; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000233; Nitr_rd_NADH; 1.
PRINTS PR00406; CYTB5RDTASE.
PR00363; CYTOCHROMEB5.
PR00407; EUMOPTERIN.
PR00371; FPNCR.
ProDom PD000612; Cyt_B5; 1.
[Domain structure / List of seq. sharing at least 1 domain]
PROSITE PS00191; CYTOCHROME_B5_1; 1.
PS50255; CYTOCHROME_B5_2; 1.
PS51384; FAD_FR; 1.
PS00559; MOLYBDOPTERIN_EUK; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P27969.
ProtoNet P27969.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
FAD; Flavoprotein; Heme; Iron; Metal-binding; Molybdenum; NAD; Nitrate assimilation; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   <1   912  >912     Nitrate reductase [NADH]. PRO_0000166057
DOMAIN   535   610  76     Cytochrome b5 heme-binding. 
DOMAIN   651   764  114     FAD-binding FR-type. 
METAL   186   186        Molybdenum-pterin (Potential). 
METAL   240   240        Molybdenum-pterin (Potential). 
METAL   570   570        Iron (heme axial ligand) (By similarity). 
METAL   593   593        Iron (heme axial ligand) (By similarity). 
DISULFID   425   425        Interchain (Potential). 
NON_TER   1     1         
Sequence information
Length: 912 AA [This is the length of the partial sequence of the unprocessed precursor] Molecular weight: 101467 Da [This is the MW of the partial sequence of the unprocessed precursor] CRC64: 47AB9C5B303190CC [This is a checksum on the sequence] 
        10         20         30         40         50         60 
SVEPRQPFGR LDAPATAPTA RAPGSNGIRR RADSPVRGCG FPSLISPPRK GPVAEEDEED 

        70         80         90        100        110        120 
DDEDDEGHED WREAYGSHLQ LEVEPSTRDP RDEGTADAWI ERNPSLIRLT GKHPLNCEPP 

       130        140        150        160        170        180 
LARLMHHGFI TPAPLHYVRN HGAVPRGDWA TWTVEVTGLV RRPARLTMDE LANGFPAAEV 

       190        200        210        220        230        240 
PATLVCAGNR RKEQNMVQQT VGFNWGAAGV STSVWRGARL RDVLLRCGVM SKKGQALNVC 

       250        260        270        280        290        300 
FEGAEDLPGG GGSKYGTSMS REWAMDPSRD IILPYAQNGE PLLPDHGYPV RVLIPGCIGG 

       310        320        330        340        350        360 
RMVKWVRRIV VTTAESDNYY HFKDNRVLPS HVDAELANAE AWWYRPEYII NELNTNSVIT 

       370        380        390        400        410        420 
TPGHDEILPI NAFTTQRAYT IKGYAYSGGG KKITRVEVTL DGGESWMLCT LDIPEKPNKY 

       430        440        450        460        470        480 
GRYWCWCFWS VEIEVLDLLG AKEVAVRAWD QTHNTQPEKL IWNLMGMMNN CWFKIKVNVC 

       490        500        510        520        530        540 
RPHKGEIGLV FEHPTQPGNQ TGGWMARQKH LETAEAAAPG LKRSTSTPFM NTAGDKQFTM 

       550        560        570        580        590        600 
SEVRKHGSKE SAWIVVHGHV YDCTAFLKDH PGGADSILIN AGSDCTEEFD AIHSDKAKAL 

       610        620        630        640        650        660 
LDTYRIGELI TTGTGYNSDN SVHGGSSLSH LAPIREATKV AGAPIALSSP REKVPCRLVD 

       670        680        690        700        710        720 
KKELSHDVRL FRFALPSSDQ VLGLPVGKHI FVCATIDGKL CMRAYTPTSM VDEIGQFELL 

       730        740        750        760        770        780 
VKVYFRDEHP KFPNGGLMTQ YLESLQVGSS SIDVKGPLGH VEYTGRGNFV INGKQRRARR 

       790        800        810        820        830        840 
LAMICGSSGI TPMYQVIQAV LRDQPEDETE MHLVYANRSE DDILLRDELD RWATEYPDRL 

       850        860        870        880        890        900 
KVWYVIDQVK RPEDGWKFSV GFVTEDILRA HVPEGGDDTL ALACGPPPMI KFAISPNLEK 

       910 
MKYDMANSFI SF
P27969 in FASTA format

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