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UniProtKB/Swiss-Prot entry P27968

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NIA7_HORVU
Primary accession number P27968
Secondary accession numbers None
Integrated into Swiss-Prot on August 1, 1992
Sequence was last modified on August 1, 1992 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 66)
Name and origin of the protein
Protein name Nitrate reductase [NAD(P)H]
Synonym EC 1.7.1.2
Gene name
Name: NAR-7
From
Hordeum vulgare (Barley) [TaxID: 4513] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Pooideae; Triticeae; Hordeum.
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=cv. Himalaya;
DOI=10.1007/BF00260624; PubMed=1896007 [NCBI, ExPASy, EBI, Israel, Japan]
Miyazaki J., Juricek M., Angelis K., Schnorr K.M., Kleinhofs A., Warner R.L.;
"Characterization and sequence of a novel nitrate reductase from barley.";
Mol. Gen. Genet. 228:329-334(1991).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X60173; CAA42739.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S16895; RDBHNP.
3D structure databases
HSSP P17571; 2CND. [HSSP ENTRY / PDB]
ModBase P27968.
Organism-specific databases
Gramene P27968; -.
Ontologies
GO
GO:0009055; Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0050660; Molecular function: FAD binding (inferred from electronic annotation from InterPro).
GO:0020037; Molecular function: heme binding (inferred from electronic annotation from InterPro).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from InterPro).
GO:0030151; Molecular function: molybdenum ion binding (inferred from electronic annotation from InterPro).
GO:0050463; Molecular function: nitrate reductase [NAD(P)H] activity (inferred from electronic annotation from EC).
GO:0042128; Biological process: nitrate assimilation (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001199; Cyt_B5.
IPR001834; Cyt_B5_reductase.
IPR001709; FPN_cyt_redctse.
IPR005066; MoCF_OxRdtse_dimer.
IPR008335; Mopterin_OxRdtase_euk.
IPR012137; Nitr_rd_NADH.
IPR008333; OxRdtase_FAD-bd.
IPR001433; OxRdtase_FAD/NAD_bd.
IPR000572; OxRdtase_Mopterin-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.10.120.10; Cyt_B5; 1.
G3DSA:2.60.40.650; MoCF_oxrdtse_dimer; 1.
G3DSA:3.90.420.10; Oxred_molyb_bd; 1.
Pfam PF00173; Cyt-b5; 1.
PF00970; FAD_binding_6; 1.
PF03404; Mo-co_dimer; 1.
PF00175; NAD_binding_1; 1.
PF00174; Oxidored_molyb; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000233; Nitr_rd_NADH; 1.
PRINTS PR00406; CYTB5RDTASE.
PR00363; CYTOCHROMEB5.
PR00407; EUMOPTERIN.
PR00371; FPNCR.
ProDom PD000612; Cyt_B5; 1.
[Domain structure / List of seq. sharing at least 1 domain]
PROSITE PS00191; CYTOCHROME_B5_1; 1.
PS50255; CYTOCHROME_B5_2; 1.
PS51384; FAD_FR; 1.
PS00559; MOLYBDOPTERIN_EUK; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P27968.
ProtoNet P27968.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
FAD; Flavoprotein; Heme; Iron; Metal-binding; Molybdenum; NAD; NADP; Nitrate assimilation; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   891  891     Nitrate reductase [NAD(P)H]. PRO_0000166058
DOMAIN   515   590  76     Cytochrome b5 heme-binding. 
DOMAIN   630   742  113     FAD-binding FR-type. 
METAL   168   168        Molybdenum-pterin (Potential). 
METAL   221   221        Molybdenum-pterin (Potential). 
METAL   550   550        Iron (heme axial ligand) (By similarity). 
METAL   573   573        Iron (heme axial ligand) (By similarity). 
DISULFID   406   406        Interchain (Potential). 
Sequence information
Length: 891 AA [This is the length of the unprocessed precursor] Molecular weight: 98630 Da [This is the MW of the unprocessed precursor] CRC64: AA47EC52FC1EFD13 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MAASVEYNRQ VSAHPWPTNA QPKAAFDLFS SSGGGRRRSG ADSDSDDEDS VPPDWRSLYS 

        70         80         90        100        110        120 
PRLDVEPSVK DPRDEATSDA WVKRHPALVR LTGKHPFNSE PPLPRLMSHG FITPVPLHYV 

       130        140        150        160        170        180 
RNHGAVPKAD WSTWTVEVTG LVKRPVKFTM EELVTGFQAV EFPVTLVCAG NRRKEQNMVR 

       190        200        210        220        230        240 
QSSGFNWGPG AISTTVWRGV RLRDVLRRCG VMGAGAASNV CFEGAEDLPG GGGCKYGTSL 

       250        260        270        280        290        300 
RRSVAMDPAR DVILAYMQNG EPLAPDHGFP VRVIVPGFIG GRMVKWLKRI VVACNESESY 

       310        320        330        340        350        360 
YHYRDNRVLP SHVDAELANA EAWWYKPECM INELNINSVI TTPGHDEVLP INALTTQKPY 

       370        380        390        400        410        420 
TMKGYAYSGG GRKVTRVEVT LDGGETWQVC DLEHPERPTK YGKYWCWCFW SVEVEVLELL 

       430        440        450        460        470        480 
GAKEMAVRAW DEALNTQPER LIWNLMGMMN NCWFRVKINV CRPHKGEIGL VFDHPTQPGN 

       490        500        510        520        530        540 
QSGGWMARQK HIETSETTQG TLKRSTSTPF MSTASAQFTM SEVRRHASKD SAWIVVHGHV 

       550        560        570        580        590        600 
YDCTAFLKDH PGGADSILIN AGSDCTEEFD AIHSAKARGL LEMYRVGELI VTGNDYSPQS 

       610        620        630        640        650        660 
SNADLAAIVE APAVVVPRLP ASAVALANPR EKVRCRLVDK KSMSHNVRLF RFALPSPDQK 

       670        680        690        700        710        720 
LGLPVGKHVY VCASTGGKLC MRAYTPTSSV EEVGHVELLI KIYFKDEDPK FPAGGLMSQY 

       730        740        750        760        770        780 
LDALPLGAPV DIKGPVGHIE YAGRGAFTVG GERRFARRLA MVAGGTGITP VYQVIQAVLR 

       790        800        810        820        830        840 
DQPDDTTEMH LVYANRTEDD MLLREEIDRW AAANPARLKV WYVVSKVGRP EDAWEYGVGR 

       850        860        870        880        890 
VDEQVLREHL PLGGDGETLA LVCGPPAMLE CTVRPGLEKM GYDLDKDCLV F
P27968 in FASTA format

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