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UniProtKB/Swiss-Prot entry P27914

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name POLG_DEN2T
Primary accession number P27914
Secondary accession numbers None
Integrated into Swiss-Prot on August 1, 1992
Sequence was last modified on August 1, 1992 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 76)
Name and origin of the protein
Protein name Genome polyprotein [Fragments]
Synonyms None
Contains Envelope protein E
Non-structural protein 1
     (NS1)
Non-structural protein 2A
     (NS2A)
Non-structural protein 2A-alpha
     (NS2A-alpha)
Serine protease subunit NS3
     (EC 3.4.21.91)
     (Non-structural protein 3)
Gene name None
From
Dengue virus type 2 (strain Tonga/EKB194/1974) (DENV-2) [TaxID: 11067] 
Taxonomy Viruses; ssRNA positive-strand viruses, no DNA stage; Flaviviridae; Flavivirus; Dengue virus group.
Virus hosts Aedimorphus [TaxID: 53540]
Diceromyia [TaxID: 53539]
Erythrocebus patas (Red guenon) (Cercopithecus patas) [TaxID: 9538]
Homo sapiens (Human) [TaxID: 9606]
Stegomyia [TaxID: 53541]
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-495.
DOI=10.1093/nar/18.19.5889; PubMed=2216784 [NCBI, ExPASy, EBI, Israel, Japan]
Chen W., Maguire T.;
"Nucleotide sequence of the envelope glycoprotein gene of a dengue-2 virus isolated during an epidemic of benign dengue fever in Tonga in 1974.";
Nucleic Acids Res. 18:5889-5889(1990).
[2]
 NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 496-1683.
Qu X., Chen W., Maguire T.;
Submitted (MAR-1991) to the EMBL/GenBank/DDBJ databases.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X54319; CAA38217.1; -; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X57469; CAA40705.1; -; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X57468; CAA40704.1; -; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR PQ0507; PQ0507.
S11482; S11482.
3D structure databases
PDB
1TG8; X-ray; 2.61 A; A=1-395.[ExPASy / RCSB / EBI]
1TGE; EM; 12.50 A; A/B/C=1-395.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1TG8; -.
1TGE; -.
SMR P27914; 1070-1246, 1236-1683.
ModBase P27914.
Ontologies
GO
GO:0016021; Cellular component: integral to membrane (inferred from electronic annotation from InterPro).
GO:0019031; Cellular component: viral envelope (inferred from electronic annotation from InterPro).
GO:0019013; Cellular component: viral nucleocapsid (inferred from electronic annotation from UniProtKB-KW).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from InterPro).
GO:0008026; Molecular function: ATP-dependent helicase activity (inferred from electronic annotation from InterPro).
GO:0003725; Molecular function: double-stranded RNA binding (inferred from electronic annotation from InterPro).
GO:0003724; Molecular function: RNA helicase activity (inferred from electronic annotation from InterPro).
GO:0005198; Molecular function: structural molecule activity (inferred from electronic annotation from InterPro).
GO:0019079; Biological process: viral genome replication (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR014001; DEAD-like_N.
IPR011492; DEAD_Flavivir.
IPR001650; DNA/RNA_helicase_C.
IPR002464; DNA/RNA_helicase_DEAH_CS.
IPR011999; Flav_glyE_cen_dm.
IPR013754; Flav_glyE_dim.
IPR001157; Flavi_NS1.
IPR000752; Flavi_NS2A.
IPR000336; Flv_glyE_Ig-like.
IPR014021; Helicase_SF1/SF2_ATP-bd.
IPR001850; Peptidase_S7.
Graphical view of domain structure.
Gene3D G3DSA:2.60.98.10; Flav_glyE_dim; 1.
G3DSA:2.60.40.350; Flv_glyE_Ig-like; 1.
Pfam PF07652; Flavi_DEAD; 1.
PF02832; Flavi_glycop_C; 1.
PF00869; Flavi_glycoprot; 1.
PF00948; Flavi_NS1; 1.
PF01005; Flavi_NS2A; 1.
PF00949; Peptidase_S7; 1.
Pfam graphical view of domain structure.
ProDom PD001496; Flavi_NS1; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00487; DEXDc; 1.
SM00490; HELICc; 1.
SMART graphical view of domain structure.
PROSITE PS00690; DEAH_ATP_HELICASE; FALSE_NEG.
PS51192; HELICASE_ATP_BIND_1; 1.
PS51194; HELICASE_CTER; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P27914.
ProtoNet P27914.
Other
LinkHub P27914; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; ATP-binding; Capsid protein; Cleavage on pair of basic residues; Core protein; Envelope protein; Glycoprotein; Helicase; Hydrolase; Membrane; Nucleotide-binding; Transmembrane; Virion.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1     495  495     Envelope protein E (By similarity). PRO_0000037980
CHAIN   496     847  352     Non-structural protein 1 (By similarity). PRO_0000037981
CHAIN   848   >1064  >217     Non-structural protein 2A (By similarity). PRO_0000308290
CHAIN   848    1035  188     Non-structural protein 2A-alpha (By similarity). PRO_0000037982
CHAIN   1066    1683  618     Serine protease subunit NS3 (By similarity). PRO_0000037983
TOPO_DOM   7     445  439     Lumenal (Potential). 
TRANSMEM   446     466  21     Potential. 
TOPO_DOM   467     472  6     Cytoplasmic (Potential). 
TRANSMEM   473     493  21     Potential. 
TOPO_DOM   494     876  383     Lumenal (Potential). 
TRANSMEM   877     897  21     Potential. 
TOPO_DOM   898   >1065  >168     Cytoplasmic (Potential). 
DOMAIN   1245    1401  157     Helicase ATP-binding. 
DOMAIN   1411    1582  172     Helicase C-terminal. 
NP_BIND   1258    1265  8     ATP (Potential). 
MOTIF   1349    1352  4     DEAH box. 
ACT_SITE   1116    1116        Charge relay system; for serine protease NS3 activity (By similarity). 
ACT_SITE   1140    1140        Charge relay system; for serine protease NS3 activity (By similarity). 
ACT_SITE   1200    1200        Charge relay system; for serine protease NS3 activity (By similarity). 
SITE   495     496  2     Cleavage; by host signal peptidase (By similarity). 
SITE   847     848  2     Cleavage; by host (By similarity). 
SITE   1035    1036  2     Cleavage; by serine protease NS3 (By similarity). 
SITE   1065    1066  2     Cleavage; by serine protease NS3 (By similarity). 
CARBOHYD   67      67        N-linked (GlcNAc...) (Potential). 
CARBOHYD   153     153        N-linked (GlcNAc...) (Potential). 
DISULFID   3      30        By similarity. 
DISULFID   60     121        By similarity. 
DISULFID   74     105        By similarity. 
DISULFID   92     116        By similarity. 
DISULFID   185     285        By similarity. 
DISULFID   302     333        By similarity. 
NON_CONS   1065    1066         
NON_TER   1       1         
NON_TER   1683    1683         
TURN   2       5  4      
STRAND   7      13  7      
STRAND   20      24  5      
STRAND   30      34  5      
STRAND   36      38  3      
STRAND   41      50  10      
STRAND   55      72  18      
STRAND   75      77  3      
HELIX   83      85  3      
STRAND   90     100  11      
HELIX   101     103  3      
STRAND   109     128  20      
STRAND   135     143  9      
TURN   148     152  5      
STRAND   159     164  6      
STRAND   171     175  5      
TURN   176     178  3      
STRAND   179     186  8      
HELIX   193     195  3      
STRAND   196     201  6      
STRAND   204     209  6      
HELIX   210     214  5      
STRAND   220     222  3      
HELIX   234     237  4      
STRAND   238     241  4      
STRAND   249     252  4      
HELIX   257     263  7      
TURN   264     266  3      
STRAND   273     276  4      
STRAND   284     288  5      
STRAND   306     310  5      
STRAND   320     326  7      
STRAND   332     334  3      
STRAND   337     340  4      
STRAND   346     353  8      
STRAND   365     370  6      
STRAND   373     381  9      
STRAND   383     385  3      
STRAND   387     393  7      
Sequence information
Length: 1683 AA [This is the length of the partial sequence of the unprocessed precursor] Molecular weight: 187440 Da [This is the MW of the partial sequence of the unprocessed precursor] CRC64: 3B0438D96196BFC8 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MRCIGISNRD FVEGVSGGSW VDIVLEHGSC VTTMAKNKPT LDFELIKTEA KQPATLRKYC 

        70         80         90        100        110        120 
IEAKLTNTTT DSRCPTQGEP TLNEEQDKRF VCKHSMVDRG WGNGCGLFGK GGIVTCAMFT 

       130        140        150        160        170        180 
CKKNMEGKIV QPENLEYTVV ITPHSGEEHA VGNDTGKHGK EVKITPQSSI TEAELTGYGT 

       190        200        210        220        230        240 
VTMECSPRTG LDFNEMVLLQ MEDKAWLVHR QWFLDLPLPW LPGADTQGSN WIQKETLVTF 

       250        260        270        280        290        300 
KNPHAKKQDV VVLGSQEGAM HTALTGATEI QMSSGNLLFT GHLKCRLRMD KLQLKGMSYS 

       310        320        330        340        350        360 
MCTGKFKIVK EIAETQHGTI VIRVQYEGDG SPCKIPFEIM DLEKRHVLGR LITVNPIVTE 

       370        380        390        400        410        420 
KDSPVNIEAE PPFGDSYIII GVEPGQLKLD WFKKGSSIGQ MFETTMRGAK RMAILGDTAW 

       430        440        450        460        470        480 
DFGSLGGVFT SIGKALHQVF GAIYGAAFSG VSWTMKILIG VIITWIGMNS RSTSLSVSLV 

       490        500        510        520        530        540 
LVGIVTLYLG VMVQADSGCV VSWKNKELKC GSGIFVTDNV HTWTEQYKFQ PESPSKLASA 

       550        560        570        580        590        600 
IQKAHEEGIC GIRSVTRLEN LMWKQITSEL NHILSENEVK LTIMTGDIKG IMQVGKRSLR 

       610        620        630        640        650        660 
PQPTELRYSW KTWGKAKMLS TELHNQTFLI DGPETAECPN TNRAWNSLEV EDYGFGVFTT 

       670        680        690        700        710        720 
NIWLRLREKQ DVFCDSKLMS AAIKDNRAVH ADMGYWIESA LNDTWKIEKA SFIEVKSCHW 

       730        740        750        760        770        780 
PKSHTLWSNG VLESEMVIPK NFAGPVSQHN NRPGYYTQTA GPWHLGKLEM DFDFCEGTTV 

       790        800        810        820        830        840 
VVTEDCGNRG PSLRTTTASG KLITEWCCRS CTLPPLRYRG EDGCWYGMEI RPLKEKEENL 

       850        860        870        880        890        900 
VSSLVTAGHG QIDNFSLGIL GMALFLEEML RTRVGTKHAI LLVAVSFVTL ITGNMSFRDL 

       910        920        930        940        950        960 
GRVMVMVGAT MTDDIGMGVT YLALLAAFRV RPTFAAGLLL RKLTSKELMM TTIGIVLLSQ 

       970        980        990       1000       1010       1020 
SSIPETILEL TDALALGMMV LKMVRNMEKY QLAVTIMAIL CVPNAVILQN AWKVSCTILA 

      1030       1040       1050       1060       1070       1080 
VVSVSPLLLT SSQQKADWIP LALTIKGLNP TAIFLTTLSR TSKKRAGVLW DVPSPPPVGK 

      1090       1100       1110       1120       1130       1140 
AELEDGAYRI KQKGILGYSQ IGAGVYKEGT FHTMWHVTRG AVLMHKGKRI EPSWADVKKD 

      1150       1160       1170       1180       1190       1200 
LISYGGGWKL EGEWKEGEEV QVLALEPGKN PRAVQTKPGL FRTNTGTIGA VSLDFSPGTS 

      1210       1220       1230       1240       1250       1260 
GSPIVDKKGK VVGLYGNGVV TRSGAYVSAI AQTEKSIEDN PEIEDDIFRK RRLTIMDLHP 

      1270       1280       1290       1300       1310       1320 
GAGKTKRYLP AIVREAIKRG LRTLILAPTR VVAAEMEEAL RGLPIRYQTP AIRAEHTGRE 

      1330       1340       1350       1360       1370       1380 
IVDLMCHATF TMRLLSPIRV PNYNLIIMDE AHFTDPASIA ARGYISTRVE MGEAAGIFMT 

      1390       1400       1410       1420       1430       1440 
ATPPGSRDPF PQSNAPIMDE EREIPERSWN SGHEWVTDFK GKTVWFVPSI KTGNDIAACL 

      1450       1460       1470       1480       1490       1500 
RKNGKRVIQL SRKTFDSEYV KTRTNDWDFV VTTDISEMGA NFKAERVIDP RRCMKPVILT 

      1510       1520       1530       1540       1550       1560 
DGEERVILAG PMPVTHSSAA QRRGRIGRNP RNENDQYIYM GEPLENDEDC AHWKEAKMLL 

      1570       1580       1590       1600       1610       1620 
DNINTPEGII PSIFEPEREK VDAIDGEYRL RGEARKTFVD LMRRGDLPVW LAYKVAAEGI 

      1630       1640       1650       1660       1670       1680 
NYADRRWCFD GTRNNQILEE NVEVEIWTKE GERKKLKPRW LDARIYSDPL ALKEFKEFAA 


GRK
P27914 in FASTA format

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View entry in raw text format (no links)
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