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UniProtKB/Swiss-Prot entry P27773


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PDIA3_MOUSE
Primary accession number P27773
Secondary accession numbers Q3TEI9 Q3TIL2 Q3UZK8 Q8C2F4 Q99LF6
Integrated into Swiss-Prot on August 1, 1992
Sequence was last modified on May 1, 2007 (Sequence version 2)
Annotations were last modified on    December 16, 2008 (Entry version 89)
Name and origin of the protein
Protein name Protein disulfide-isomerase A3 [Precursor]
Synonyms EC 5.3.4.1
Disulfide isomerase ER-60
ERp60
58 kDa microsomal protein
p58
ERp57
Gene name
Name: Pdia3
Synonyms: Erp, Erp60, Grp58
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=2033248 [NCBI, ExPASy, EBI, Israel, Japan]
Hempel W.M., Defranco A.L.;
"Expression of phospholipase C isozymes by murine B lymphocytes.";
J. Immunol. 146:3713-3720(1991).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=C57BL/6;
Wang W., Jaiswal A.K.;
"Mouse GRP58 gene structure.";
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=C57BL/6J, DBA/2, and NOD;
TISSUE=Thymus;
DOI=10.1126/science.1112014; PubMed=16141072 [NCBI, ExPASy, EBI, Israel, Japan]
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
"The transcriptional landscape of the mammalian genome.";
Science 309:1559-1563(2005).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=C57BL/6J;
The mouse genome sequencing consortium;
Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=Czech II, and FVB/N;
TISSUE=Mammary tumor;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[6]
PROTEIN SEQUENCE OF 25-41 AND 306-329.
TISSUE=Fibroblast;
DOI=10.1002/elps.11501501101; PubMed=7523108 [NCBI, ExPASy, EBI, Israel, Japan]
Merrick B.A., Patterson R.M., Wichter L.L., He C., Selkirk J.K.;
"Separation and sequencing of familiar and novel murine proteins using preparative two-dimensional gel electrophoresis.";
Electrophoresis 15:735-745(1994).
[7]
PROTEIN SEQUENCE OF 25-41, AND PHOSPHORYLATION.
TISSUE=Fibroblast;
Merrick B.A., Wichter L.L., Patterson R.M., He C., Selkirk J.K.;
"Identification of the two isoforms of phospholipase C-alpha from dividing murine fibroblasts by protein microsequencing.";
Biochem. Arch. 9:335-340(1993).
[8]
PROTEIN SEQUENCE OF 63-73; 94-103; 119-128; 130-139; 147-172; 183-193; 234-251; 258-270; 335-343; 347-361; 366-378; 448-459 AND 482-495, AND MASS SPECTROMETRY.
STRAIN=C57BL/6;
TISSUE=Brain;
Lubec G., Kang S.U.;
Submitted (APR-2007) to UniProtKB.
[9]
PROTEIN SEQUENCE OF 195-214; 306-329; 348-362 AND 472-482, AND MASS SPECTROMETRY.
TISSUE=Brain, and Hippocampus;
Lubec G., Klug S., Yang J.W., Zigmond M.;
Submitted (JUL-2007) to UniProtKB.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M73329; AAA39944.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
DQ000491; AAY16987.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK088721; BAC40527.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK133799; BAE21849.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK167807; BAE39834.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK169611; BAE41259.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL845466; CAM24231.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC003285; AAH03285.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC033439; AAH33439.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_031978.2; -.
UniGene Mm.263177
3D structure databases
HSSP P07237; 1MEK. [HSSP ENTRY / PDB]
SMR P27773; 25-137, 134-365, 357-488.
ModBase P27773.
PTM databases
PhosphoSite P27773; -.
2D gel databases
SWISS-2DPAGE P27773; -.
REPRODUCTION-2DPAGE P27773; -.
Q3TEI9; -.
Q8C2F4; -.
Organism-specific databases
MGI MGI:95834; Pdia3.
Gene expression databases
ArrayExpress P27773; -.
CleanEx MM_PDIA3; -.
GermOnline ENSMUSG00000027248; Mus musculus.
Ontologies
GO
GO:0005783; Cellular component: endoplasmic reticulum (inferred from direct assay from MGI).
GO:0005788; Cellular component: endoplasmic reticulum lumen (inferred from electronic annotation from UniProtKB-SubCell).
GO:0042470; Cellular component: melanosome (inferred from electronic annotation from UniProtKB-SubCell).
GO:0003756; Molecular function: protein disulfide isomerase activity (inferred from electronic annotation from EC).
GO:0045454; Biological process: cell redox homeostasis (inferred from electronic annotation from InterPro).
GO:0043065; Biological process: positive regulation of apoptosis (inferred from mutant phenotype from MGI).
QuickGo view.
Family and domain databases
InterPro IPR005788; Disulph_isom.
IPR005792; Disulphide_isom.
IPR006662; Thioredoxin-like.
IPR013766; Thioredoxin_dom.
IPR012335; Thioredoxin_fold.
Graphical view of domain structure.
Gene3D G3DSA:3.40.30.10; Thioredoxin_fold; 1.
Pfam PF00085; Thioredoxin; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR01130; ER_PDI_fam; 1.
TIGR01126; pdi_dom; 2.
PROSITE PS00194; THIOREDOXIN_1; 2.
PS51352; THIOREDOXIN_2; 2.
PROSITE graphical view of domain structure (profiles).
Proteomics databases
PRIDE P27773; -.
Genome annotation databases
Ensembl ENSMUSG00000027248; Mus musculus. [Contig view]
GeneID 14827; -.
KEGG mmu:14827; -.
Phylogenomic databases
HOGENOM P27773; -.
HOVERGEN P27773; -.
Other
NextBio 287035; -.
SOURCE Pdia3; Mus musculus.
ProtoNet P27773.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Direct protein sequencing; Endoplasmic reticulum; Isomerase; Phosphoprotein; Redox-active center; Repeat; Signal.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    24  24      
CHAIN   25   505  481     Protein disulfide-isomerase A3. PRO_0000034226
DOMAIN   25   133  109     Thioredoxin 1. 
DOMAIN   343   485  143     Thioredoxin 2. 
MOTIF   502   505  4     Prevents secretion from ER (By similarity). 
ACT_SITE   57    57        Nucleophile (By similarity). 
ACT_SITE   60    60        Nucleophile (By similarity). 
ACT_SITE   406   406        Nucleophile (By similarity). 
ACT_SITE   409   409        Nucleophile (By similarity). 
SITE   58    58  1     Contributes to redox potential value (By similarity). 
SITE   59    59  1     Contributes to redox potential value (By similarity). 
SITE   119   119  1     Lowers pKa of C-terminal Cys of first active site (By similarity). 
SITE   407   407  1     Contributes to redox potential value (By similarity). 
SITE   408   408  1     Contributes to redox potential value (By similarity). 
SITE   471   471  1     Lowers pKa of C-terminal Cys of second active site (By similarity). 
DISULFID   57    60        Redox-active (By similarity). 
DISULFID   406   409        Redox-active (By similarity). 
CONFLICT   20    20        R -> C (in Ref. 3; BAC40527/BAE41259). 
CONFLICT   76    76        Missing (in Ref. 1; AAA39944). 
CONFLICT   108   108        D -> A (in Ref. 1; AAA39944). 
CONFLICT   230   230        G -> A (in Ref. 1; AAA39944). 
CONFLICT   264   264        Y -> F (in Ref. 3; BAE39834). 
CONFLICT   315   315        G -> S (in Ref. 1; AAA39944). 
CONFLICT   387   387        D -> G (in Ref. 3; BAE39834). 
Sequence information
Length: 505 AA [This is the length of the unprocessed precursor] Molecular weight: 56678 Da [This is the MW of the unprocessed precursor] CRC64: 3A7CD1C35981C4B3 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MRFSCLALLP GVALLLASAR LAAASDVLEL TDENFESRVS DTGSAGLMLV EFFAPWCGHC 

        70         80         90        100        110        120 
KRLAPEYEAA ATRLKGIVPL AKVDCTANTN TCNKYGVSGY PTLKIFRDGE EAGAYDGPRT 

       130        140        150        160        170        180 
ADGIVSHLKK QAGPASVPLR TEEEFKKFIS DKDASVVGFF RDLFSDGHSE FLKAASNLRD 

       190        200        210        220        230        240 
NYRFAHTNIE SLVKEYDDNG EGITIFRPLH LANKFEDKTV AYTEKKMTSG KIKKFIQDSI 

       250        260        270        280        290        300 
FGLCPHMTED NKDLIQGKDL LTAYYDVDYE KNAKGSNYWR NRVMMVAKKF LDAGHKLNFA 

       310        320        330        340        350        360 
VASRKTFSHE LSDFGLESTT GEVPVVAIRT AKGEKFVMQE EFSRDGKALE QFLQEYFDGN 

       370        380        390        400        410        420 
LKRYLKSEPI PESNEGPVKV VVAENFDDIV NEEDKDVLIE FYAPWCGHCK NLEPKYKELG 

       430        440        450        460        470        480 
EKLSKDPNIV IAKMDATAND VPSPYEVKGF PTIYFSPANK KLTPKKYEGG RELNDFISYL 

       490        500 
QREATNPPII QEEKPKKKKK AQEDL 

P27773 in FASTA format

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