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UniProtKB/Swiss-Prot entry P27743

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ACVS_NOCLA
Primary accession number P27743
Secondary accession numbers None
Integrated into Swiss-Prot on August 1, 1992
Sequence was last modified on August 1, 1992 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 59)
Name and origin of the protein
Protein name N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase
Synonyms EC 6.3.2.26
Delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase
ACV synthetase
ACVS
Gene name
Name: pcbAB
From
Nocardia lactamdurans [TaxID: 1913] 
Taxonomy Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; Pseudonocardineae; Pseudonocardiaceae; Amycolatopsis.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=VAR LC 411;
DOI=10.1111/j.1365-2958.1991.tb01885.x; PubMed=1956290 [NCBI, ExPASy, EBI, Israel, Japan]
Coque J.J.R., Martin J.F., Calzada J.G., Liras P.;
"The cephamycin biosynthetic genes pcbAB, encoding a large multidomain peptide synthetase, and pcbC of Nocardia lactamdurans are clustered together in an organization different from the same genes in Acremonium chrysogenum and Penicillium chrysogenum.";
Mol. Microbiol. 5:1125-1133(1991).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X57310; CAA40561.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S18268; S18268.
3D structure databases
HSSP O30409; 1DNY. [HSSP ENTRY / PDB]
ModBase P27743.
Ontologies
GO
GO:0000036; Molecular function: acyl carrier activity (inferred from electronic annotation from InterPro).
GO:0048037; Molecular function: cofactor binding (inferred from electronic annotation from InterPro).
GO:0016788; Molecular function: hydrolase activity, acting on ester bonds (inferred from electronic annotation from InterPro).
GO:0050564; Molecular function: N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase activity (inferred from electronic annotation from EC).
GO:0031177; Molecular function: phosphopantetheine binding (inferred from electronic annotation from InterPro).
GO:0017000; Biological process: antibiotic biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR010071; AA_adenyl_dom.
IPR009081; ACP_like.
IPR000873; AMP-dep_Synth/Lig.
IPR001242; Condensatn.
IPR006163; Phsphopanteth_bd.
IPR006162; Ppantne_S.
IPR001031; Thioesterase.
Graphical view of domain structure.
Gene3D G3DSA:1.10.1200.10; ACP_like; 1.
Pfam PF00501; AMP-binding; 3.
PF00668; Condensation; 3.
PF00550; PP-binding; 3.
PF00975; Thioesterase; 1.
Pfam graphical view of domain structure.
PRINTS PR00154; AMPBINDING.
TIGRFAMs TIGR01733; AA-adenyl-dom; 3.
PROSITE PS50075; ACP_DOMAIN; 3.
PS00455; AMP_BINDING; 1.
PS00012; PHOSPHOPANTETHEINE; 2.
PROSITE graphical view of domain structure (profiles).
BLOCKS P27743.
ProtoNet P27743.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Antibiotic biosynthesis; Ligase; Multifunctional enzyme; Phosphopantetheine; Repeat.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   3649  3649     N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase. PRO_0000193059
DOMAIN   788    857  70     Acyl carrier 1. 
DOMAIN   1864   1933  70     Acyl carrier 2. 
DOMAIN   2910   2981  72     Acyl carrier 3. 
REGION   401    861  461     Domain 1 (adipate-activating). 
REGION   1014   1937  924     Domain 2 (cysteine-activating). 
REGION   2079   2985  907     Domain 3 (valine-activating). 
ACT_SITE   3502   3502        For thioesterase activity (By similarity). 
BINDING   820    820        Phosphopantetheine (covalent) (By similarity). 
BINDING   1896   1896        Phosphopantetheine (covalent) (By similarity). 
BINDING   2944   2944        Phosphopantetheine (covalent) (By similarity). 
Sequence information
Length: 3649 AA [This is the length of the unprocessed precursor] Molecular weight: 404088 Da [This is the MW of the unprocessed precursor] CRC64: 6FD095704F858E6B [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MTSARHLKSA ADWCARIDAI AGQRCDLEML LKDEWRHRVA VRDSDTAVRA TQEKELTISG 

        70         80         90        100        110        120 
QDYTALKQAL GAMPLEAFAL ATLHSVLHAY GHGHQTVVAF LRDGKVLPVV VDHLEQAGLT 

       130        140        150        160        170        180 
CAEAAEQLED AVAREDMYLP PEELLQRGLF DALLVLADGH LGFTELPPAP LVTIVRDDPA 

       190        200        210        220        230        240 
AGCLHWRIAY AGEFFEDKII AGVLDVAREV LGQFIGRPEQ LVADIDLVSA EQELQLHQWN 

       250        260        270        280        290        300 
GTDGEFDEDK RLNELFEDVV RRAPDREAVV CGDVRLTYRE VNERANQFAH WLIQGPVRVR 

       310        320        330        340        350        360 
PGALIGLYLD KSDLGVVATF GIWKSGAAYV PIDPAYPAER IRFLVGDTGL SGIVTNRRHA 

       370        380        390        400        410        420 
ERLREVLGDE HASVHVIEVE AVVAGPHPEQ ARENPGLALS SRDRAYVTYT SGTTGVPKGV 

       430        440        450        460        470        480 
PKYHYSVVNS ITDLSERYDM RRPGTERVAL FASYVFEPHL RQTLIALINE QTLVIVPDDV 

       490        500        510        520        530        540 
RLDPDLFPEY IERHGVTYLN ATGSVLQHFD LRRCASLKRL LLVGEELTAS GLRQLREKFA 

       550        560        570        580        590        600 
GRVVNEYAFT EAAFVTAVKE FGPGVTERRD RSIGRPLRNV KWYVLSQGLK QLPIGAIGEL 

       610        620        630        640        650        660 
YIGGCGVAPG YLNRDDLTAE RFTANPFQTE EEKARGRNGR LYRTGDLARV LLNGEVEFMG 

       670        680        690        700        710        720 
RADFQLKLNG VRVEPGEIEA QATEFPGVKK CVVVAKENAT GDRHLVGYYL VEDGAEVAEA 

       730        740        750        760        770        780 
DLIAFLEQRL IRIMVPARMV RLTSIPVNVN GKVDWRALPD VSLHPAPANA MNGALLAIDG 

       790        800        810        820        830        840 
SNAPLLAITE QLRAIWSEVL GVPQNRIGER DDFFRLGGQS ISCILLIARV RQRLSLSLGV 

       850        860        870        880        890        900 
EDVFALRTLD ALAGHLESQG HAEPEVVAEE VTTGSEPVRV LANGLQQGLL YHHLKTAGGD 

       910        920        930        940        950        960 
DAYVVQSVHR YHAPIRPELM KDAWQAARQT YPALRLRFDW AEEPVQIVDN DDKPFDWRFV 

       970        980        990       1000       1010       1020 
DLSATADDAE QEARVRELQE RDRTEPYDLA GGRLFRVYLI KQREDLFSLI FSCHHIILDG 

      1030       1040       1050       1060       1070       1080 
WSLPVLHDEV HRNYLALRAG QPIESDVDNA YVAAQRYWEA HRNDHAAYWV EQLGRIDERG 

      1090       1100       1110       1120       1130       1140 
DFAGLLNEKS RYRVSLGDYD HVQRHRTRKL YLGADLTGAL KAGCAADQVT LHSVLQFVWH 

      1150       1160       1170       1180       1190       1200 
KVLHAIGGGN TTVVGTIVSG RNLPVDGIEN SAGLFINTLP LIVDHDQQAG QNVAEAVRDI 

      1210       1220       1230       1240       1250       1260 
QAAVNTMNSK SIVELGRLQS GEMKRRLFDT LLVLENYPRL LDEEEELAHQ EALRFEKAYD 

      1270       1280       1290       1300       1310       1320 
ADKVDYPIAV VAREEGDELT VTLWYAGELF DEDTIDTLLD VARTLFRQVT EDIARPVREL 

      1330       1340       1350       1360       1370       1380 
DLISPSMRAR FDSWNETAEE FPADKTLHAV FEEMAERWPD EIAVVYRENR LTYRELNERA 

      1390       1400       1410       1420       1430       1440 
NRLAHYLRSV VELRPDDLVA LVLDKSELMI TAIIAAWKTG AAYVPIDSGY PDDRISFMLS 

      1450       1460       1470       1480       1490       1500 
DTAARVVVTN EIHSDRLRSL AETGTPVLEI ELLHLDDQPA VNPVTETTST DLAYAIYTSG 

      1510       1520       1530       1540       1550       1560 
TTGKPKAVLV EHRGVVNLQV SLAKLFGLDK AHRDEALLSF SNYIFDHFVE QMTDALLNGQ 

      1570       1580       1590       1600       1610       1620 
KLVVLDDSMR TDPGRLCRYM NDEQVTYLSG TPSVLSLYDY SSATSLTRID AIGEDFTEPV 

      1630       1640       1650       1660       1670       1680 
FAKIRGTFPG LIINGYGPTE ISITSHKRPY PPDVHRVNKS IGFPVANTKC HVLNKAMKPV 

      1690       1700       1710       1720       1730       1740 
PVGGIGELYI GGIGVTRGYL NREDLTADRF VENPFQTAEE RRLGENGRLY KTGDLVRWLP 

      1750       1760       1770       1780       1790       1800 
NGEVEYLGRT DLQVKIRGQR VELGEVEAAL SSYPGVVRSL VVAREHAVGQ KYLVGFYVGE 

      1810       1820       1830       1840       1850       1860 
QEFDEQDLKQ WMRKKLPESV VPARVLRITD IPVTPSGKLD ARRLPETDFG AGEGAEYVAP 

      1870       1880       1890       1900       1910       1920 
VSEFELKLCG IWAQVLEIAP DRIGVHDDFF ALGGDSIRAM ALAQAITTGF GQGLGVATVL 

      1930       1940       1950       1960       1970       1980 
QHTTLAAQAE HIQAAALEHT AWTPPPTAVE HPPVSLAQER LLFIDDFEGG TAAYNIPFVL 

      1990       2000       2010       2020       2030       2040 
RLPAHTRAAL PGALGTLVRR HPALRTLLKT DDQGVRRQYP IPADDVRLEV PSTTVDSRAE 

      2050       2060       2070       2080       2090       2100 
LDEVLTERAG YVFRLHEELP IRAEAFDHGD EIYLSVVVHH SCFDGWSWDI FRRELAALLD 

      2110       2120       2130       2140       2150       2160 
GVPEADLGAL RGTYGEFAVW QRQYLTGKRL AALTEFWTGA LGGFETIALP LDHPRPPRFD 

      2170       2180       2190       2200       2210       2220 
YRGRELEFEL DERTTEALRE LARTARVSLY SVLLGAWCLM LNMYTGQHDL VVGTPSANRG 

      2230       2240       2250       2260       2270       2280 
RPEFDRAVGF FANLLALRVR VDPAATLPAY VRSVGEAVVA AQVHGELPFE QLVKELKVEK 

      2290       2300       2310       2320       2330       2340 
DPSRHPILQL NFTLQNVSDH TSALTGYQPD SGGWTTTKFD LSATMTETAT GLAGNLTYAA 

      2350       2360       2370       2380       2390       2400 
SLFDDTSASG FIATFKHVLA EFASAAAQTP IAQLTALDEP GQAALPDATR RARRPGGPGR 

      2410       2420       2430       2440       2450       2460 
CTRLFEEVAA TWPDRVAVVH GDVRLTYREL NERANRLAHH LRSVAEPRAD ELIALVLDKS 

      2470       2480       2490       2500       2510       2520 
ELTLVAILAV WKAGAAYMPI DPSYPDDRIA FMLSDTGAKL VLAGEAHGSR VRGLTSGDVL 

      2530       2540       2550       2560       2570       2580 
DLEQLDLTGE PAENPVTETT STELAYAIYT SGTTGKPKAV LVSHGSVDSF RAQLSGRYFG 

      2590       2600       2610       2620       2630       2640 
SPDESAEAVL FLANYVFDFS VEQLALSVLG GHKLLVPPPS AADDPAFYEL ANREGLSYLS 

      2650       2660       2670       2680       2690       2700 
GTPTQVERFD LARLSHLRCV LVAGEAFQPQ HFEKMRGEFA GPILNAYGTT ETTVYNTVHR 

      2710       2720       2730       2740       2750       2760 
FEPGDAYRNT LGAPLGNTRL YVLGDGMKLL PTGAVGELYL AGDCVTEGYL HRPELTRERF 

      2770       2780       2790       2800       2810       2820 
LPNPFAAESG RFPMIYRTGD VVRRGPDGEL QYLGRNDAQV KINGLRIEPG EVEAALAGCS 

      2830       2840       2850       2860       2870       2880 
GVRQCAVVAG ADPQAPERKR LVGYYLPEPG AAVDEADLFA ALRAQLMPSM VPSLLVRLDR 

      2890       2900       2910       2920       2930       2940 
PLPMTITGKL DVDALPSADF SPKRAAYAAP RDRVEARLCH LWSAQLPGGT VGIDDDFFRC 

      2950       2960       2970       2980       2990       3000 
GGDSISALHL ASQVQREIER KVSVKYLFDH PTVRSFVDNV LSGLAESSGD DEPEQGRLTG 

      3010       3020       3030       3040       3050       3060 
ECPMLPIQEW FFAKPLADRH RWNHNFAIRT PPLDPGELRT ALDRLVEHHD AFRLRFPESG 

      3070       3080       3090       3100       3110       3120 
GEVYAEDAAP ITLHELDVRG LADADLRQRL VDWQRTFDLA NGPTACAAYL HGFDDGTARV 

      3130       3140       3150       3160       3170       3180 
WFALHHLVVD TVSWHILAQD LEILYNGGDL GAKTGSYRQW AQAVRDYTPA EGEREFWAET 

      3190       3200       3210       3220       3230       3240 
TRDMESAELL AQTEGTTRRR EEFALTAPDT RTLLAESPWA YDTEVNDLLL TATGFALRSI 

      3250       3260       3270       3280       3290       3300 
TRQATNHLTV EGHGRELFEG APDVRDTVGW FTTMHPFAVE VDPGDLGRSV LATRANRRRV 

      3310       3320       3330       3340       3350       3360 
PHHGIGYGAL FGGEAPLPAV SFNYLGRLGE GDGQPTEAWQ LDPALSGSHT VDGNRLANRS 

      3370       3380       3390       3400       3410       3420 
SIDVTMSCTG GRLVAVVDSL LGEAATRLFA SELKVWLERL VSHTATVARN EPAREATTEL 

      3430       3440       3450       3460       3470       3480 
FDPYILVNED AERTLFVLPP GEGGAESYLS NLARQLPDLR LVLFNNVHLH TPMGSFEELG 

      3490       3500       3510       3520       3530       3540 
RYYVEHIRRL QPSGPYHLLG WSFGGVLSLE ISRQLARAGE RIDDLLLIDP YFGMRQASAN 

      3550       3560       3570       3580       3590       3600 
IGLPGVEDIL DPINYHYRPD EADLARLAGR LGNLVLFKAG EPNDVVNGPH QPRLFEYYHG 

      3610       3620       3630       3640 
TRFNHLDLLL PAAAIEVCDL AGETHHSWVR NEKLVRLMCE RISTSLGSD
P27743 in FASTA format

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