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UniProtKB/Swiss-Prot entry P27742

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ACVS_EMENI
Primary accession number P27742
Secondary accession numbers None
Integrated into Swiss-Prot on August 1, 1992
Sequence was last modified on August 1, 1992 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 61)
Name and origin of the protein
Protein name N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase
Synonyms EC 6.3.2.26
Delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase
ACV synthetase
ACVS
Gene name
Name: acvA
From
Emericella nidulans (Aspergillus nidulans) [TaxID: 162425] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Trichocomaceae; Emericella.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
STRAIN=G191;
PubMed=2061333 [NCBI, ExPASy, EBI, Israel, Japan]
Maccabe A.P., van Liempt H., Pallissa H., Unkles S.E., Riach M.B.R., Pfeifer E., von Doehren H., Kinghorn J.R.;
"Delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Aspergillus nidulans. Molecular characterization of the acvA gene encoding the first enzyme of the penicillin biosynthetic pathway.";
J. Biol. Chem. 266:12646-12654(1991).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X54853; CAA38631.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A40889; A40889.
3D structure databases
HSSP P14687; 1AMU. [HSSP ENTRY / PDB]
ModBase P27742.
Ontologies
GO
GO:0000036; Molecular function: acyl carrier activity (inferred from electronic annotation from InterPro).
GO:0048037; Molecular function: cofactor binding (inferred from electronic annotation from InterPro).
GO:0016788; Molecular function: hydrolase activity, acting on ester bonds (inferred from electronic annotation from InterPro).
GO:0050564; Molecular function: N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase activity (inferred from electronic annotation from EC).
GO:0031177; Molecular function: phosphopantetheine binding (inferred from electronic annotation from InterPro).
GO:0017000; Biological process: antibiotic biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR010071; AA_adenyl_dom.
IPR009081; ACP_like.
IPR000873; AMP-dep_Synth/Lig.
IPR001242; Condensatn.
IPR006163; Phsphopanteth_bd.
IPR006162; Ppantne_S.
IPR001031; Thioesterase.
Graphical view of domain structure.
Gene3D G3DSA:1.10.1200.10; ACP_like; 3.
Pfam PF00501; AMP-binding; 3.
PF00668; Condensation; 3.
PF00550; PP-binding; 3.
PF00975; Thioesterase; 1.
Pfam graphical view of domain structure.
PRINTS PR00154; AMPBINDING.
TIGRFAMs TIGR01733; AA-adenyl-dom; 3.
PROSITE PS50075; ACP_DOMAIN; 3.
PS00455; AMP_BINDING; 3.
PS00012; PHOSPHOPANTETHEINE; 3.
PROSITE graphical view of domain structure (profiles).
BLOCKS P27742.
ProtoNet P27742.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Antibiotic biosynthesis; Direct protein sequencing; Ligase; Multifunctional enzyme; Phosphopantetheine; Repeat.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   3770  3770     N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase. PRO_0000193057
DOMAIN   850    919  70     Acyl carrier 1. 
DOMAIN   1929   2002  74     Acyl carrier 2. 
DOMAIN   3020   3087  68     Acyl carrier 3. 
REGION   321    910  590     Domain 1 (adipate-activating). 
REGION   1413   1993  581     Domain 2 (cysteine-activating). 
REGION   2494   3078  585     Domain 3 (valine-activating). 
ACT_SITE   3623   3623        For thioesterase activity (By similarity). 
BINDING   882    882        Phosphopantetheine (covalent) (By similarity). 
BINDING   1965   1965        Phosphopantetheine (covalent) (By similarity). 
BINDING   3050   3050        Phosphopantetheine (covalent) (By similarity). 
Sequence information
Length: 3770 AA [This is the length of the unprocessed precursor] Molecular weight: 422456 Da [This is the MW of the unprocessed precursor] CRC64: CB66B6D232A58CB0 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MSPPGLLSED GPGYSGGYAD PTVPKVNWKQ SNGKSAGGNG DVDAGNGNID PSKSGVGVQV 

        70         80         90        100        110        120 
CFAGGLEGWK AGISKITERC DLSSIATNST KYQLAVTGFS DGPDDYNEYS VPFPSEVLVA 

       130        140        150        160        170        180 
MEEMCLARDI SMRSVIQFAV HYVLKGFGGG SHTVAASIDV GDDPNNIATS YTITPSIVCH 

       190        200        210        220        230        240 
ESRQGQTVMQ EIQSMEKLNQ LRKQEMHPGE AGLSLIRMGL FDILVIFADA NKCEGLIAGL 

       250        260        270        280        290        300 
PLAVMVCEGG GRLQVRIHFS GSLFRQKTLV DIAEALNVLF AKAASGGATP VRDLELLSAE 

       310        320        330        340        350        360 
QKQQLEEWNK TDGEYPECKR LNHLIEEATQ LHEDKVAIVY KRRQLTYGEL NAQANCFAHY 

       370        380        390        400        410        420 
LRSIGILPEQ LVALFLEKSE NLIVTILGIW KSGAAYVPID PTYPDERVRF VLEDTQAKVI 

       430        440        450        460        470        480 
IASNHLAERL QSEVISDREL SIIRLEHCLS AIDQQPSTFP RANLRDPSLT SKQLAYVTYT 

       490        500        510        520        530        540 
SGTTGFPKGI LKQHTNVVNS ITDLSARYGV TGDHHEAILL FSAYVFEPFV RQMLMALVNG 

       550        560        570        580        590        600 
HLLAMVDDAE KYDAEKLIPF IREHKITYLN GTASVLQEYD FSSCPSLKRL ILVGENLTES 

       610        620        630        640        650        660 
RYLALRRHFK NCILNEYGFT ESAFVTALNV FEPGSARNNT SLGRPVRNVK CYILNKSLKR 

       670        680        690        700        710        720 
VPIGATGELH IGGLGISKGY LNRPDLTPQR FIPNPFQTDH EKELGLNQLM YKTGDLARWL 

       730        740        750        760        770        780 
PNGEIEYLGR ADFQIKLRGI RIEPGEIEST LAGYPGVRTS LVVSKRLRHG EKETTNEHLV 

       790        800        810        820        830        840 
GYYVGDNTSV SETALLQFLE LKLPRYMIPT RLVRVSQIPV TVNGKADLRA LPSVDLIQPK 

       850        860        870        880        890        900 
VSSCELTDEV EIALGKIWAD VLGAHHLSIS RKDNFFRLGG HSITCIQLIA RIRQQLGVII 

       910        920        930        940        950        960 
SIEDVFSSRT LERMAELLRS KESNGTPDER ARPQLKTVAG EVANANVYLA NSLQQGFVYQ 

       970        980        990       1000       1010       1020 
FLKNMGRSEA YVMQSVLRYD VNINPDLFKK AWKQVQHMLP TLRLRFQWGQ DVLQVIDEDQ 

      1030       1040       1050       1060       1070       1080 
PLNWWFLHLA DDSALPEEQK LLELQRRDLA EPYDLAAGSL FRIYLIEHSS TRFSCLFSCH 

      1090       1100       1110       1120       1130       1140 
HAILDGWSLP LLFRKTHGTY LHLLHGHSLR TLEDPYRQSQ QYLQDHREDH LRYWAGIVNQ 

      1150       1160       1170       1180       1190       1200 
IEERCDMNAL LNERSRYKIQ LADYDKVEDQ QQLTLTVPDA SWLSKLRQTC SAQGITLHSI 

      1210       1220       1230       1240       1250       1260 
LQFVWHAVLH AYGGGTHTVT GTTISGRNLP VSGIERSVGL YINTLPLVIN QLAYKNKTVL 

      1270       1280       1290       1300       1310       1320 
EAIRDVQAIV NGMNSRGNVE LGRLQKNELK HGLFDSLFVL ENYPILDKSE EMRQKSELKY 

      1330       1340       1350       1360       1370       1380 
TIEGNIEKLD YPLAVIAREV DLTGGFTFTI CYARELFDEI VISELLQMVR DTLLQVAKHL 

      1390       1400       1410       1420       1430       1440 
DDPVRSLEYL SSAQMAQLDA WNATDAEFPD TTLHAMFEKE AAQKPDKVAV VYEQRSLTYR 

      1450       1460       1470       1480       1490       1500 
QLNERANRMA HQLKSDISPK PNSIIALVVD KSEHMIATIL AVWKTGGAYV PIDPEYPDDR 

      1510       1520       1530       1540       1550       1560 
IRYILEDTSA IAVISDACYL SRIQELAGES VRLYRSDIST QTDGNWSVSN PAPSSTSTDL 

      1570       1580       1590       1600       1610       1620 
AYIIYTSGTT GKPKGVMVEH HGVVNLQISL SKTFGLRDTD DEVILSFSNY VFDHFVEQMT 

      1630       1640       1650       1660       1670       1680 
DAILNGQTLV MLNDAMRSDK ERLYQYIETN RVTYLSGTPS VISMYEFSRF KDHLRRVDCV 

      1690       1700       1710       1720       1730       1740 
GEAFSQPVFD QIRDTFQGLI INGYGPTEIS ITTHKRLYPF PERRTDKSIG QQIGNSTSYV 

      1750       1760       1770       1780       1790       1800 
LNADMKRVPI GAVGELYLGG EGVARGYHNR PEVTAERFLR NPFQTDSERQ NGRNSRLYRT 

      1810       1820       1830       1840       1850       1860 
GDLVRWIPGS NGEIEYLGRN DFQVKIRGLR IELGEIEAVM SSHPDIKQSV VIAKSGKEGD 

      1870       1880       1890       1900       1910       1920 
QKFLVGYFVA SSPLSPGAIR RFMQSRLPGY MIPSSFIPIS SLPVTPSGKL DTKALPTAEE 

      1930       1940       1950       1960       1970       1980 
KGAMNVLAPR NEIESILCGI SAGLLDISAQ TIGSDSDFFT LGGDSLKSTK LSFKIHEVFG 

      1990       2000       2010       2020       2030       2040 
RTISVSALFR HRTIESLAHL IMNNVGDIQE ITPVDYDNRR KIAVSPAQER LLFIHELEGG 

      2050       2060       2070       2080       2090       2100 
GNAYNIDAAF ELPPYIDQSR VEEALYTILS RHEALRTFLL RDQATGTFYQ KILTTDEAKC 

      2110       2120       2130       2140       2150       2160 
MLIIEKSAVS TIDQIDSIVG RLSQHIFRLD SELPWLAHIV THKTGNLYLT LSFHHTCFDA 

      2170       2180       2190       2200       2210       2220 
WSLKIFEREL RVFCASKQKG GNMPILPMPQ VQYKEYAEHH RRRLGKNQIQ KLSDFWLQRL 

      2230       2240       2250       2260       2270       2280 
DGLEPLQLLP DYPRPAQFNY DGGDLSVILD GVVLETLRGI AKDHGVTLYA VLLAVYCLML 

      2290       2300       2310       2320       2330       2340 
STYTHQVDIA VGVPISHRTH PLFQSIVGFF VNMVVVRVDV KDFAVHDLIR RVMKPLVDAQ 

      2350       2360       2370       2380       2390       2400 
LHQDMPFQDV TKLLRVDNDA SRHPLVQTVF NFESDMDKEF ETTPSIQDTA TIAPYQSVQR 

      2410       2420       2430       2440       2450       2460 
IKSVAKFDLN ATATESGSAL KINFNYATSL FRKETIQGFL ETYRHLLLQL SYLGSQGLKE 

      2470       2480       2490       2500       2510       2520 
DTKLLLVRPE EMSGPHLPLA GLSNGAETLE AISLSRAFEF EAFRVPDRAA VVQGDKSLSY 

      2530       2540       2550       2560       2570       2580 
TELNKRANQL ARYIQSVAHL RPDDKVLLIL DKSIDMIICI LAIWKTGSAY VPLDPSYPKE 

      2590       2600       2610       2620       2630       2640 
RVQCISEVVQ AKILITESRY ASAWGSQTST ILAIDSPKVS NMVNNQATHN LPNIAGIKNL 

      2650       2660       2670       2680       2690       2700 
AYIIFTSGTS GKPKGVLVEQ GGVLHLRDAL RKRYFGIECN EYHAVLFLSN YVFDFSIEQL 

      2710       2720       2730       2740       2750       2760 
VLSIMSGHKL IIPEGEFVAD DEFYITANGQ RLSYLSGTPS LLQQIDLARL NHLQVVTAAG 

      2770       2780       2790       2800       2810       2820 
EQLHAAQFNK LRSGFRGPIY NAYGITETTV YNIVSEFSAQ SQFENALREL LPGTRAYLLN 

      2830       2840       2850       2860       2870       2880 
HATQPVPMNA VGELYLAGDC VARGYLNQPV LTGDRFIQNP FQTEQDIACG SYPRLYRTGD 

      2890       2900       2910       2920       2930       2940 
LFRCRLDRQH QPYLEYLGRA DLQVKIRGYR IEPSEVQNVL ASCPGVRECA VVAKYENTDA 

      2950       2960       2970       2980       2990       3000 
YSRIAKFLVG YYTPDTETVS DSSILAHMKS KLPAYMVPKY LCRLEGGLPV TINGKLDVRK 

      3010       3020       3030       3040       3050       3060 
LPDIGNPQHQ ISYNPPRDVL EADLCRLWAS ALGTERCGID DDLFRLGGDS ITALHLAAQI 

      3070       3080       3090       3100       3110       3120 
HHQIGRKVTV RDIFDHPTIR GIHDNVMVKL VPHNVPQFQA EQQTVLGDAP LLPIQIWFLS 

      3130       3140       3150       3160       3170       3180 
KSLQHPSHWN HTFYLRTPDL DVTTLSTAVA ELQLYHDAFR MRLRQIDGRT VQCFADDISP 

      3190       3200       3210       3220       3230       3240 
VQLRVLNVKD VDGSAAIDQQ LQKYQSDFDL EKGPICAAAY LHGYEDRSAR VWFSVHHIII 

      3250       3260       3270       3280       3290       3300 
DIVSWQILAR DLQILYEGGT LGRKSSSVRQ WAEALQSYQG SASERAYWEG LLAQTAANIS 

      3310       3320       3330       3340       3350       3360 
ALPPVTGTRT RLARTWSDDR TVILLNEASN QNASIQDLLL AAVGLALQQV TPGSPSMITL 

      3370       3380       3390       3400       3410       3420 
EGHGREEIVD PTLDLSRTLG WFTSMYPFEI PPLNVETLSQ GIASLRECLR QVPARGIGFG 

      3430       3440       3450       3460       3470       3480 
SLYGYCKHQM PQVTFNYLGQ LTSKQSITDQ WALAVGDGEM QYGLTTSPAD RDQSSFAVDI 

      3490       3500       3510       3520       3530       3540 
TASCVNGALS VEMNSAWSLE KSMRFISRIE EVLNMILSGT LAQQATPVLT PQVFNEEMYT 

      3550       3560       3570       3580       3590       3600 
PYFEFSKTPR RGPILFLLPP GEGGAESYFN NIVKHLPTTN MVVFNNYYLH SKSLNTFEKL 

      3610       3620       3630       3640       3650       3660 
AEMYLGHIRQ IQPDGPYHFI GWSFGGTIAM EISRQLVGLG STIGLLGIID TYFNVPGATR 

      3670       3680       3690       3700       3710       3720 
AIGLGDTEVL DPIHHISQPE PADFQCLPAS TDYIILFKAT RVNDKFQSEN QRRLYEYYDK 

      3730       3740       3750       3760       3770 
TLLNDLDWLL PGASNIHLVR LEEDTHFSWA TNPRQIAHVC STIEKFLARY
P27742 in FASTA format

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