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UniProtKB/Swiss-Prot entry P27625

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name RPC1_PLAFA
Primary accession number P27625
Secondary accession numbers None
Integrated into Swiss-Prot on August 1, 1992
Sequence was last modified on August 1, 1992 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 52)
Name and origin of the protein
Protein name DNA-directed RNA polymerase III subunit RPC1
Synonyms RNA polymerase III subunit C1
EC 2.7.7.6
DNA-directed RNA polymerase III largest subunit
C160
Gene name None
From
Plasmodium falciparum [TaxID: 5833] 
Taxonomy Eukaryota; Alveolata; Apicomplexa; Aconoidasida; Haemosporida; Plasmodium; Plasmodium (Laverania).
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0166-6851(91)90047-A; PubMed=1656254 [NCBI, ExPASy, EBI, Israel, Japan]
Li W.B., Bzik D.J., Tanaka M., Gu H., Fox B.A., Inselburg J.;
"Characterization of the gene encoding the largest subunit of Plasmodium falciparum RNA polymerase III.";
Mol. Biochem. Parasitol. 46:229-240(1991).
Comments
  • FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).
  • CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
  • SUBUNIT: Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity).
  • SUBCELLULAR LOCATION: Nucleus (By similarity).
  • SIMILARITY: Belongs to the RNA polymerase beta' chain family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M73770; AAA29729.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A45597; A45597.
3D structure databases
HSSP P04050; 1K83. [HSSP ENTRY / PDB]
ModBase P27625.
Ontologies
GO
GO:0005634; Cellular component: nucleus (inferred from electronic annotation from UniProtKB-KW).
GO:0003677; Molecular function: DNA binding (inferred from electronic annotation from InterPro).
GO:0003899; Molecular function: DNA-directed RNA polymerase activity (inferred from electronic annotation from InterPro).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006350; Biological process: transcription (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR015700; RNA_pol3.
IPR000722; RNA_pol_A.
IPR006592; RNA_pol_N.
IPR007080; RNA_pol_Rpb1_1.
IPR007066; RNA_pol_Rpb1_3.
IPR007083; RNA_pol_Rpb1_4.
IPR007081; RNA_pol_Rpb1_5.
Graphical view of domain structure.
Gene3D G3DSA:2.40.40.30; RNA_pol_A; 1.
G3DSA:3.90.1120.10; RNA_pol_Rpb1_1; 1.
PANTHER PTHR19376:SF15; RNA_pol3; 1.
Pfam PF04997; RNA_pol_Rpb1_1; 1.
PF00623; RNA_pol_Rpb1_2; 1.
PF04983; RNA_pol_Rpb1_3; 1.
PF05000; RNA_pol_Rpb1_4; 1.
PF04998; RNA_pol_Rpb1_5; 1.
Pfam graphical view of domain structure.
SMART SM00663; RPOLA_N; 1.
SMART graphical view of domain structure.
BLOCKS P27625.
ProtoNet P27625.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
DNA-directed RNA polymerase; Magnesium; Metal-binding; Nucleotidyltransferase; Nucleus; Transcription; Transferase; Zinc; Zinc-finger.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   2339  2339     DNA-directed RNA polymerase III subunit RPC1. PRO_0000073949
ZN_FING   88    101  14     C3H1-type (Potential). 
REGION   955    967  13     Bridging helix (By similarity). 
METAL   88     88        Zinc 1 (By similarity). 
METAL   91     91        Zinc 1 (By similarity). 
METAL   98     98        Zinc 1 (By similarity). 
METAL   101    101        Zinc 1 (By similarity). 
METAL   128    128        Zinc 2 (By similarity). 
METAL   131    131        Zinc 2 (By similarity). 
METAL   175    175        Zinc 2 (By similarity). 
METAL   611    611        Magnesium; catalytic (By similarity). 
METAL   613    613        Magnesium; catalytic (By similarity). 
METAL   615    615        Magnesium; catalytic (By similarity). 
Sequence information
Length: 2339 AA [This is the length of the unprocessed precursor] Molecular weight: 272832 Da [This is the MW of the unprocessed precursor] CRC64: EDD899363086FD48 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MMKKKNIDIE ELKRLIEESS MKKRFVKDIK RNCEIKSIRF GIMSKEDIIK YSEVKIMNRE 

        70         80         90        100        110        120 
MYKNNSGIPY PYGVLDLKLG AHKSNSVCET CNKKLINCSG HFGYIELNYP VFHIGYYKYI 

       130        140        150        160        170        180 
IHILYCICKY CSSLLLSKEK IDFYCNLKKK STDDSFYKKH LFKRILNNCK KVNKCYICGN 

       190        200        210        220        230        240 
PQGVIKKIIK PSLDQFMKLK HILKVKENGK MIIKEEDLNS LYVLKLFKNI NPYHVKLLNI 

       250        260        270        280        290        300 
ENPEKLIITA LLVPPNTIRP SVIIDEHGTA EDDLTCILSE ITQLNNTIYN QCTNGYQTNQ 

       310        320        330        340        350        360 
FLGNVEFLQL QITRFINSDS PAVSQLLATQ NISKPGRGIC QRLKGKEGRF RCNLSGKRVD 

       370        380        390        400        410        420 
FSSRTVISPD PNISIDEVVI PKIIAMRLTY PETVNKYNID KLKMLIKNGC NKWPGANYII 

       430        440        450        460        470        480 
KKSKKGTDPY SDISTSYNNN SNNISSIGCS NIFNVVNNYI NNNCKNVRYN IKDVNNNVLL 

       490        500        510        520        530        540 
KDMCDINNMN NDINNNINNI YKNTSETNLC NVNNHNNNNN IYCNNQTQDN EEERKNSQFN 

       550        560        570        580        590        600 
KISLKYANKN HVIQNLNIGD VVERHICDGD IVLFNRQPSL HRMSIMCHKA KIMDFKTFRF 

       610        620        630        640        650        660 
NECVCSPYNA DFDGDEMNLD VPQTEEARAE ALYLMNVKHN LITPKNGEVI IALTQDFLSA 

       670        680        690        700        710        720 
SYIITNKDTF LDRDTFCLLC SYFSDASLYI ELPIPAILKP KELWTGKQLI SVLIKPNKKE 

       730        740        750        760        770        780 
NTIINFEIQE REYSNKFGDL KHLCLNDSYV CFYKSELICG SLGKKVLGSS KYGLFYYLIH 

       790        800        810        820        830        840 
HNSSHIALKI MNRLSKLTSR YFSNKGMTIG IDDVRPSQTL TEKKKDLLLK GYEKVNNEII 

       850        860        870        880        890        900 
LYNEKKMQIQ PGCTLEETLE IKVKSILDDL RNDAGKTCNQ YLHYLNKPLI MFNSGAKGAL 

       910        920        930        940        950        960 
INIAQMIACV GQQNVAGQRI QNGFINRTLP HFHFHCKDSE SRGFVQNSFY TGLSPTEFFF 

       970        980        990       1000       1010       1020 
HTMSGREGLV DTAVKTAETG YMQRRLMKAL EDLSIHYDYS VRSCDKQIVQ FIYGDDALNP 

      1030       1040       1050       1060       1070       1080 
SYIDNNNTYL DQFDKVFDHI VSISSSHLLL SYKNKIPYLP HVQHQNKTSN MNNIYNNMNN 

      1090       1100       1110       1120       1130       1140 
INNNDSNRSI IYNNDSNMNN INNNDSNMNS IHNNNSNMNN IHNNDSNRSI IHNNDSNMNS 

      1150       1160       1170       1180       1190       1200 
IHNNDSNMNS IHNNNSNMNN IHNNDSNRSI IHNNDSNMNS IHNNDSNNNN NYKDCTHNPY 

      1210       1220       1230       1240       1250       1260 
ICNESLIIRN IMNRLIYQNI AQEDLFIPLE HDEFLVNKIM ESYTDQECNY EDIIRSLDLN 

      1270       1280       1290       1300       1310       1320 
KNVSYIHNDQ GKHLSLQMCA EEHITINNTN NDNTYVEQIE MKELSKNKTK EKQSFKGTIR 

      1330       1340       1350       1360       1370       1380 
DMHEDSEEQM NKFITKKAKF FIEKKKGKMH ECNDDIEYNN TQYDNIQYNN ISCNYIKSQN 

      1390       1400       1410       1420       1430       1440 
LENTHHQVNN DLSFIKNNVI LPPKEYHSIF HFVNDYRNVV EIKNLMDKKK IFLNNSEKNV 

      1450       1460       1470       1480       1490       1500 
VQSKYNRMSK NLKKKIEIIN NIYRNEKKKL NRWKTKMDND DNYWSSDDDS IIAKKIIKIK 

      1510       1520       1530       1540       1550       1560 
NKEKRKYHPK EEKENFDRNN YKMITDNNNN DNNNNNNDNN NNDNNNNNNN SNNNNYYYNL 

      1570       1580       1590       1600       1610       1620 
HDDVNNLGVT NYNTNIYPND CNGIYEKETN NNELTTNSNM CDKNNDFSDE FFNNINENDL 

      1630       1640       1650       1660       1670       1680 
LYDNKYYRQI FKNVIGFVSV FEYVESYKQH YILFPYEIIK WTSFLLEYLT EIIPTNIFLH 

      1690       1700       1710       1720       1730       1740 
TKLSKKEKPT HQKNTGKMKI YIEEIKKWLF IKAINIYKYF SFKKSIELIK KKDYFNYIIK 

      1750       1760       1770       1780       1790       1800 
NYDISHRYII HDYSFINLKQ LYLFIFFNIY KYFKYISTPG DAVGSISAQS IGEPGTQMTL 

      1810       1820       1830       1840       1850       1860 
KTFHFAGVAS MNVTLGVPRI KEIINASNSI QTPILNIPLE VNDNYNFALM MKSKLEKTTI 

      1870       1880       1890       1900       1910       1920 
RDICMYIKED YTSRGVFLSV KFNEELIQKL FLNINAYNIK DIILKQSHIN KIKINKIHIN 

      1930       1940       1950       1960       1970       1980 
VINKYKLHIS LKNDEFIFFQ MESLKKGLLD LLIYGDKDIK RCIIKKEDIE VTDNEDEICD 

      1990       2000       2010       2020       2030       2040 
DMDEYYNVSQ GTELYERKCN SKEENKNAIR VKKEEIDDNL EKEENIIYVS EKDSVNQLKS 

      2050       2060       2070       2080       2090       2100 
EKKKDINDDN NNNDDNNNNN DDDNKINDTI FNDDIDSDRN NLKENGSKLE NVGEHIIERL 

      2110       2120       2130       2140       2150       2160 
SYKMKEKNVK KEHIKKEPNL INLDTINLDT LNFDEINVHN INNEKIEFYD EHLNICQGNK 

      2170       2180       2190       2200       2210       2220 
KHIQKKKKKK TVYSILVEGN SLNYVLGLEG VDFKHIISNH VINVFQVLGI EAARITIINE 

      2230       2240       2250       2260       2270       2280 
IKKCVEAYSI DIDIRHIMLL ADIMAFTGDI LGINRFGIQK ARQSTLMLAS FEETNEHLFV 

      2290       2300       2310       2320       2330 
SSFFKNVDEI NNISESIIVG KNIPIGTGAF QLLYDYKLEK ETKNLTLLEK AERETAMNY
P27625 in FASTA format

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