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UniProtKB/Swiss-Prot entry P27448


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MARK3_HUMAN
Primary accession number P27448
Secondary accession numbers O60219 Q8TB41 Q8WX83 Q96RG1 Q9UMY9 Q9UN34
Integrated into Swiss-Prot on August 1, 1992
Sequence was last modified on February 28, 2003 (Sequence version 2)
Annotations were last modified on    September 23, 2008 (Entry version 92)
Name and origin of the protein
Protein name MAP/microtubule affinity-regulating kinase 3
Synonyms EC 2.7.11.1
Cdc25C-associated protein kinase 1
C-TAK1
cTAK1
Serine/threonine protein kinase p78
Ser/Thr protein kinase PAR-1
Protein kinase STK10
Gene name
Name: MARK3
Synonyms: CTAK1
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
PubMed=9543386 [NCBI, ExPASy, EBI, Israel, Japan]
Peng C.Y., Graves P.R., Ogg S., Thoma R.S., Byrnes M.J. III, Wu Z., Stephenson M.T., Piwnica-Worms H.;
"C-TAK1 protein kinase phosphorylates human Cdc25C on serine 216 and promotes 14-3-3 protein binding.";
Cell Growth Differ. 9:197-208(1998).
[2]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Waggoner S.N., Stephen R., Farrar W.L., Howard O.M.Z.;
"Human serine/threonine protein kinase cTAK1/Kp78/Mark3: Identification of a novel splice variant and a larger 5'UTR.";
Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
DOI=10.1038/35083016; PubMed=11433294 [NCBI, ExPASy, EBI, Israel, Japan]
Sun T.-Q., Lu B., Feng J.-J., Reinhard C., Jan Y.N., Fantl W.J., Williams L.T.;
"PAR-1 is a Dishevelled-associated kinase and a positive regulator of Wnt signalling.";
Nat. Cell Biol. 3:628-636(2001).
[4]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5).
Drewes G.;
"Characterization of an alternatively spliced form of MARK3 from human brain.";
Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases.
[5]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 6).
Maheshwari K.K., Som S., Parsa I.;
Submitted (JAN-1992) to the EMBL/GenBank/DDBJ databases.
[6]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
TISSUE=Cervix;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[7]
NUCLEOTIDE SEQUENCE [MRNA] OF 323-775 (ISOFORM 7).
TISSUE=Urinary bladder;
Reynolds C.H., Patel U.A., Anderton B.H.;
"Homo sapiens mRNA partial sequence for a protein kinase, STK10, similar to p78/C-TAK1.";
Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
[8]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-234; SER-442; SER-492 AND SER-624, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.T600062-MCP200; PubMed=17192257 [NCBI, ExPASy, EBI, Israel, Japan]
Wissing J., Jaensch L., Nimtz M., Dieterich G., Hornberger R., Keri G., Wehland J., Daub H.;
"Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry.";
Mol. Cell. Proteomics 6:537-547(2007).
[9]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-563; SER-566; THR-572 AND THR-614, AND MASS SPECTROMETRY.
DOI=10.1021/pr0705441; PubMed=18220336 [NCBI, ExPASy, EBI, Israel, Japan]
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III;
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.";
J. Proteome Res. 7:1346-1351(2008).
[10]
VARIANT [LARGE SCALE ANALYSIS] ALA-452.
DOI=10.1038/nature05610; PubMed=17344846 [NCBI, ExPASy, EBI, Israel, Japan]
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G., Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.;
"Patterns of somatic mutation in human cancer genomes.";
Nature 446:153-158(2007).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U64205; AAC15093.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF159295; AAD48007.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF387637; AAK82367.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF465413; AAL69982.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M80359; AAA59991.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC024773; AAH24773.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF170723; AAD51631.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S27966; S27966.
RefSeq NP_001122390.1; -.
NP_001122391.1; -.
NP_001122392.1; -.
NP_002367.4; -.
UniGene Hs.35828
3D structure databases
HSSP Q63450; 1A06. [HSSP ENTRY / PDB]
SMR P27448; 680-776.
ModBase P27448.
Protein-protein interaction databases
IntAct P27448; -.
PTM databases
PhosphoSite P27448; -.
Organism-specific databases
H-InvDB HIX0012000; -.
HGNC HGNC:6897; MARK3.
GenAtlas MARK3.
MIM 602678; gene. [NCBI / EBI]
PharmGKB PA30640; -.
GeneCards P27448.
Gene expression databases
ArrayExpress P27448; -.
CleanEx HS_MARK3; -.
GermOnline ENSG00000075413; Homo sapiens.
Ontologies
GO
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR001772; Kinase_KA1.
IPR000719; Prot_kinase_core.
IPR017441; Protein_kinase_ATP_bd_CS.
IPR017442; Se/Thr_pkinase-rel.
IPR008271; Ser_thr_pkin_AS.
IPR002290; Ser_thr_pkinase.
IPR000449; UBA/transl_elong_EF1B_N.
IPR015940; UBA/transl_elong_EF1B_N_euk.
Graphical view of domain structure.
Pfam PF02149; KA1; 1.
PF00069; Pkinase; 1.
PF00627; UBA; 1.
Pfam graphical view of domain structure.
ProDom PD000001; Prot_kinase; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00220; S_TKc; 1.
SM00165; UBA; 1.
SMART graphical view of domain structure.
PROSITE PS50032; KA1; 1.
PS00107; PROTEIN_KINASE_ATP; 1.
PS50011; PROTEIN_KINASE_DOM; 1.
PS00108; PROTEIN_KINASE_ST; 1.
PS50030; UBA; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P27448.
Genome annotation databases
Ensembl ENSG00000075413; Homo sapiens. [Contig view]
GeneID 4140; -.
Phylogenomic databases
HOVERGEN P27448; -.
Other
LinkHub P27448; -.
SOURCE MARK3; Homo sapiens.
ProtoNet P27448.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; ATP-binding; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Serine/threonine-protein kinase; Transferase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   776  776     MAP/microtubule affinity-regulating kinase 3. PRO_0000086304
DOMAIN   56   330  275     Protein kinase. 
DOMAIN   326   365  40     UBA. 
DOMAIN   727   776  50     KA1. 
NP_BIND   62    70  9     ATP (By similarity). 
ACT_SITE   201   201        Proton acceptor (By similarity). 
BINDING   85    85        ATP (By similarity). 
MOD_RES   234   234        Phosphothreonine. 
MOD_RES   442   442        Phosphoserine. 
MOD_RES   492   492        Phosphoserine. 
MOD_RES   563   563        Phosphoserine. 
MOD_RES   566   566        Phosphoserine. 
MOD_RES   572   572        Phosphothreonine. 
MOD_RES   614   614        Phosphothreonine. 
MOD_RES   624   624        Phosphoserine. 
VAR_SEQ   162   184        Missing (in isoform 3, isoform 4, isoform 5 and isoform 6). VSP_004942
VAR_SEQ   394   409        Missing (in isoform 6 and isoform 7). VSP_004943
VAR_SEQ   638   661        Missing (in isoform 2, isoform 3 and isoform 6). VSP_004944
VAR_SEQ   638   646        Missing (in isoform 4). VSP_004945
VARIANT   452   452  1     V -> A. VAR_040765 
CONFLICT   125   125        E -> Q (in Ref. 5; AAA59991). 
CONFLICT   139   139        E -> K (in Ref. 4 and 5). 
CONFLICT   149   149        R -> K (in Ref. 2; AAD48007). 
CONFLICT   448   448        A -> G (in Ref. 5; AAA59991). 
CONFLICT   466   466        S -> G (in Ref. 1 and 2). 
CONFLICT   479   479        S -> T (in Ref. 5; AAA59991). 
CONFLICT   539   539        A -> D (in Ref. 5; AAA59991). 
CONFLICT   626   626        N -> T (in Ref. 5; AAA59991). 
CONFLICT   668   668        E -> K (in Ref. 5; AAA59991). 
Sequence information
Length: 776 AA [This is the length of the unprocessed precursor] Molecular weight: 86944 Da [This is the MW of the unprocessed precursor] CRC64: A245496849070098 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSTRTPLPTV NERDTENHTS HGDGRQEVTS RTSRSGARCR NSIASCADEQ PHIGNYRLLK 

        70         80         90        100        110        120 
TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL FREVRIMKIL NHPNIVKLFE 

       130        140        150        160        170        180 
VIETEKTLYL IMEYASGGEV FDYLVAHGRM KEKEARSKFR QGCQAGQTIK VQVSFDLLSL 

       190        200        210        220        230        240 
MFTFIVSAVQ YCHQKRIVHR DLKAENLLLD ADMNIKIADF GFSNEFTVGG KLDTFCGSPP 

       250        260        270        280        290        300 
YAAPELFQGK KYDGPEVDVW SLGVILYTLV SGSLPFDGQN LKELRERVLR GKYRIPFYMS 

       310        320        330        340        350        360 
TDCENLLKRF LVLNPIKRGT LEQIMKDRWI NAGHEEDELK PFVEPELDIS DQKRIDIMVG 

       370        380        390        400        410        420 
MGYSQEEIQE SLSKMKYDEI TATYLLLGRK SSELDASDSS SSSNLSLAKV RPSSDLNNST 

       430        440        450        460        470        480 
GQSPHHKVQR SVSSSQKQRR YSDHAGPAIP SVVAYPKRSQ TSTADSDLKE DGISSRKSSG 

       490        500        510        520        530        540 
SAVGGKGIAP ASPMLGNASN PNKADIPERK KSSTVPSSNT ASGGMTRRNT YVCSERTTAD 

       550        560        570        580        590        600 
RHSVIQNGKE NSTIPDQRTP VASTHSISSA ATPDRIRFPR GTASRSTFHG QPRERRTATY 

       610        620        630        640        650        660 
NGPPASPSLS HEATPLSQTR SRGSTNLFSK LTSKLTRRNM SFRFIKRLPT EYERNGRYEG 

       670        680        690        700        710        720 
SSRNVSAEQK DENKEAKPRS LRFTWSMKTT SSMDPGDMMR EIRKVLDANN CDYEQRERFL 

       730        740        750        760        770 
LFCVHGDGHA ENLVQWEMEV CKLPRLSLNG VRFKRISGTS IAFKNIASKI ANELKL 

P27448 in FASTA format

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