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UniProtKB/Swiss-Prot entry P27395

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name POLG_JAEV1
Primary accession number P27395
Secondary accession numbers Q82920 Q82921 Q82922 Q82923 Q82924 Q82925 Q82926 Q82927 Q82928
Integrated into Swiss-Prot on August 1, 1992
Sequence was last modified on August 1, 1992 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 75)
Name and origin of the protein
Protein name Genome polyprotein
Synonyms None
Contains Protein C
     (Core protein)
     (Capsid protein)
Small envelope protein M
     (Matrix protein)
Envelope protein E
Non-structural protein 1
     (NS1)
Non-structural protein 2A
     (NS2A)
Flavivirin protease NS2B regulatory subunit
Flavivirin protease NS3 catalytic subunit
     (EC 3.4.21.91)
Non-structural protein 4A
     (NS4A)
Non-structural protein 4B
     (NS4B)
RNA-directed RNA polymerase
     (EC 2.7.7.48)
     (NS5)
Gene name None
From
Japanese encephalitis virus (strain SA-14) [TaxID: 11073] 
Taxonomy Viruses; ssRNA positive-strand viruses, no DNA stage; Flaviviridae; Flavivirus; Japanese encephalitis virus group.
Virus hosts Ardeidae (herons) [TaxID: 8899]
Bos taurus (Bovine) [TaxID: 9913]
Culex gelidus [TaxID: 308713]
Culex tritaeniorhynchus [TaxID: 7178]
Equus caballus (Horse) [TaxID: 9796]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1016/0042-6822(90)90519-W; PubMed=2371768 [NCBI, ExPASy, EBI, Israel, Japan]
Nitayaphan S., Grant J.A., Chang G.J.J., Trent D.W.;
"Nucleotide sequence of the virulent SA-14 strain of Japanese encephalitis virus and its attenuated vaccine derivative, SA-14-14-2.";
Virology 177:541-552(1990).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M55506; AAA46248.1; -; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M55506; AAA46249.1; ALT_INIT; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A35519; GNWVJS.
3D structure databases
SMR P27395; 295-693, 1514-1680, 2533-2794.
ModBase P27395.
Ontologies
GO
GO:0016021; Cellular component: integral to membrane (inferred from electronic annotation from InterPro).
GO:0019031; Cellular component: viral envelope (inferred from electronic annotation from InterPro).
GO:0019013; Cellular component: viral nucleocapsid (inferred from electronic annotation from UniProtKB-KW).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from InterPro).
GO:0008026; Molecular function: ATP-dependent helicase activity (inferred from electronic annotation from InterPro).
GO:0003725; Molecular function: double-stranded RNA binding (inferred from electronic annotation from InterPro).
GO:0003724; Molecular function: RNA helicase activity (inferred from electronic annotation from InterPro).
GO:0003968; Molecular function: RNA-directed RNA polymerase activity (inferred from electronic annotation from InterPro).
GO:0004252; Molecular function: serine-type endopeptidase activity (inferred from electronic annotation from InterPro).
GO:0005198; Molecular function: structural molecule activity (inferred from electronic annotation from InterPro).
GO:0016070; Biological process: RNA metabolic process (inferred from electronic annotation from InterPro).
GO:0006410; Biological process: transcription, RNA-dependent (inferred from electronic annotation from UniProtKB-KW).
GO:0019079; Biological process: viral genome replication (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR014001; DEAD-like_N.
IPR011492; DEAD_Flavivir.
IPR001650; DNA/RNA_helicase_C.
IPR002464; DNA/RNA_helicase_DEAH_CS.
IPR013756; Flav_glyE_cen_2.
IPR011999; Flav_glyE_cen_dm.
IPR013754; Flav_glyE_dim.
IPR001122; Flavi_capsidC.
IPR000069; Flavi_M.
IPR001157; Flavi_NS1.
IPR000752; Flavi_NS2A.
IPR000487; Flavi_NS2B.
IPR000404; Flavi_NS4A.
IPR001528; Flavi_NS4B.
IPR002535; Flavi_propep.
IPR000336; Flv_glyE_Ig-like.
IPR014412; Gen_Poly_FLV.
IPR014021; Helicase_SF1/SF2_ATP-bd.
IPR001850; Peptidase_S7.
IPR000208; RNA_pol_flaviviral.
IPR007094; RNA_pol_PSvir.
IPR002877; RrmJFtsJ_MeTrfase.
Graphical view of domain structure.
Gene3D G3DSA:3.30.67.10; Flav_glyE_cen_2; 1.
G3DSA:2.60.98.10; Flav_glyE_dim; 1.
G3DSA:2.60.40.350; Flv_glyE_Ig-like; 1.
Pfam PF01003; Flavi_capsid; 1.
PF07652; Flavi_DEAD; 1.
PF02832; Flavi_glycop_C; 1.
PF00869; Flavi_glycoprot; 1.
PF01004; Flavi_M; 1.
PF00948; Flavi_NS1; 1.
PF01005; Flavi_NS2A; 1.
PF01002; Flavi_NS2B; 1.
PF01350; Flavi_NS4A; 1.
PF01349; Flavi_NS4B; 1.
PF00972; Flavi_NS5; 1.
PF01570; Flavi_propep; 1.
PF01728; FtsJ; 1.
PF00271; Helicase_C; 1.
PF00949; Peptidase_S7; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF003817; Gen_Poly_FLV; 1.
ProDom PD001496; Flavi_NS1; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00487; DEXDc; 1.
SM00490; HELICc; 1.
SMART graphical view of domain structure.
PROSITE PS00690; DEAH_ATP_HELICASE; FALSE_NEG.
PS51192; HELICASE_ATP_BIND_1; 1.
PS51194; HELICASE_CTER; 1.
PS50507; RDRP_SSRNA_POS; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P27395.
ProtoNet P27395.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Capsid protein; Cleavage on pair of basic residues; Complete proteome; Core protein; Envelope protein; Glycoprotein; Helicase; Hydrolase; Membrane; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; Transferase; Transmembrane; Virion.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
INIT_MET   1      1        Removed; by host. 
CHAIN   2    127  126     Protein C. PRO_0000037836
PROPEP   128    219  92      PRO_0000037837
CHAIN   220    294  75     Small envelope protein M. PRO_0000037838
CHAIN   295    794  500     Envelope protein E. PRO_0000037839
CHAIN   795   1206  412     Non-structural protein 1. PRO_0000037840
CHAIN   1207   1373  167     Non-structural protein 2A. PRO_0000037841
CHAIN   1374   1504  131     Flavivirin protease NS2B regulatory subunit. PRO_0000037842
CHAIN   1505   2123  619     Flavivirin protease NS3 catalytic subunit. PRO_0000037843
CHAIN   2124   2412  289     Non-structural protein 4A. PRO_0000037844
CHAIN   2413   2527  115     Non-structural protein 4B. PRO_0000037845
CHAIN   2528   3432  905     RNA-directed RNA polymerase. PRO_0000037846
TRANSMEM   44     60  17     Potential. 
TRANSMEM   112    127  16     Potential. 
TRANSMEM   280    294  15     Potential. 
TRANSMEM   774    790  17     Potential. 
DOMAIN   1685   1841  157     Helicase ATP-binding. 
DOMAIN   1852   2017  166     Helicase C-terminal. 
DOMAIN   3057   3209  153     RdRp catalytic. 
NP_BIND   1698   1705  8     ATP (Potential). 
MOTIF   1789   1792  4     DEAH box. 
ACT_SITE   1555   1555        Charge relay system (By similarity). 
ACT_SITE   1579   1579        Charge relay system (By similarity). 
ACT_SITE   1639   1639        Charge relay system (By similarity). 
CARBOHYD   142    142        N-linked (GlcNAc...) (Potential). 
CARBOHYD   448    448        N-linked (GlcNAc...) (Potential). 
CARBOHYD   924    924        N-linked (GlcNAc...) (Potential). 
CARBOHYD   1001   1001        N-linked (GlcNAc...) (Potential). 
CARBOHYD   1594   1594        N-linked (GlcNAc...) (Potential). 
CARBOHYD   2463   2463        N-linked (GlcNAc...) (Potential). 
CARBOHYD   2491   2491        N-linked (GlcNAc...) (Potential). 
CARBOHYD   2761   2761        N-linked (GlcNAc...) (Potential). 
CARBOHYD   2866   2866        N-linked (GlcNAc...) (Potential). 
CARBOHYD   2904   2904        N-linked (GlcNAc...) (Potential). 
DISULFID   297    324        By similarity. 
DISULFID   354    410        By similarity. 
DISULFID   368    399        By similarity. 
DISULFID   386    415        By similarity. 
DISULFID   484    581        By similarity. 
DISULFID   598    629        By similarity. 
Sequence information
Length: 3432 AA [This is the length of the unprocessed precursor] Molecular weight: 380211 Da [This is the MW of the unprocessed precursor] CRC64: 11B9423735B1B5FE [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MTKKPGGPGK NRAINMLKRG LPRVFPLVGV KRVVMSLLDG RGPVRFVLAL ITFFKFTALA 

        70         80         90        100        110        120 
PTKALLGRWK AVEKSVAMKH LTSFKRELGT LIDAVNKRGR KQNKRGGNEG SIMWLASLAV 

       130        140        150        160        170        180 
VIACAGAMKL SNFQGKLLMT INNTDIADVI VIPTSKGENR CWVRAIDVGY MCEDTITYEC 

       190        200        210        220        230        240 
PKLTMGNDPE DVDCWCDNQE VYVQYGRCTR TRHSKRSRRS VSVQTHGESS LVNKKEAWLD 

       250        260        270        280        290        300 
STKATRYLMK TENWIIRNPG YAFLAAVLGW MLGSNNGQRV VFTILLLLVA PAYSFNCLGM 

       310        320        330        340        350        360 
GNRDFIEGAS GATWVDLVLE GDSCLTIMAN DKPTLDVRMI NIEASQLAEV RSYCYHASVT 

       370        380        390        400        410        420 
DISTVARCPT TGEAHNEKRA DSSYVCKQGF TDRGWGNGCG LFGKGSIDTC AKFSCTSKAI 

       430        440        450        460        470        480 
GRTIQPENIK YEVGIFVHGT TTSENHGNYS AQVGASQAAK FTVTPNAPSI TLKLGDYGEV 

       490        500        510        520        530        540 
TLDCEPRSGL NTEAFYVMTV GSKSFLVHRE WFHDLALPWT SPSSTAWRNR ELLMEFEGAH 

       550        560        570        580        590        600 
ATKQSVVALG SQEGGLHQAL AGAIVVEYSS SVKLTSGHLK CRLKMDKLAL KGTTYGMCTE 

       610        620        630        640        650        660 
KFSFAKNPVD TGHGTVVIEL SYSGSDGPCK IPIVSVASLN DMTPVGRLVT VNPFVATSSA 

       670        680        690        700        710        720 
NSKVLVEMEP PFGDSYIVVG RGDKQINHHW HKAGSTLGKA FSTTLKGAQR LAALGDTAWD 

       730        740        750        760        770        780 
FGSIGGVFNS IGRAVHQVFG GAFRTLFGGM SWITQGLMGA LLLWMGVNAR DRSIALAFLA 

       790        800        810        820        830        840 
TGGVLVFLAT NVHADTGCAI DITRKEMRCG SGIFVHNDVE AWVDRYKYLP ETPRSLAKIV 

       850        860        870        880        890        900 
HKAHKEGVCG VRSVTRLEHQ MWEAVRDELN VLLKENAVDL SVVVNKPVGR YRSAPKRLSM 

       910        920        930        940        950        960 
TQEKFEMGWK AWGKSILFAP ELANSTFVVD GPETKECPDE HRAWNSMQIE DFGFGITSTR 

       970        980        990       1000       1010       1020 
VWLKIREEST DECDGAIIGT AVKGHVAVHS DLSYWIESRY NDTWKLERAV FGEVKSCTWP 

      1030       1040       1050       1060       1070       1080 
ETHTLWGDDV EESELIIPHT IAGPKSKHNR REGYKTQNQG PWDENGIVLD FDYCPGTKVT 

      1090       1100       1110       1120       1130       1140 
ITEDCSKRGP SVRTTTDSGK LITDWCCRSC SLPPLRFRTE NGCWYGMEIR PVMHDETTLV 

      1150       1160       1170       1180       1190       1200 
RSQVDAFKGE MVDPFQLGLL VMFLATQEVL RKRWTARLTI PAVLGVLLVL MLGGITYTDL 

      1210       1220       1230       1240       1250       1260 
ARYVVLVAAA FAEANSGGDV LHLALIAVFK IQPAFLVMNM LSTRWTNQEN VILVLGAAFF 

      1270       1280       1290       1300       1310       1320 
QLASVDLQIG VHGILNAAAI AWMIVRAITF PTTSSVTMPV LALLTPGMRA LYLDTYRIIL 

      1330       1340       1350       1360       1370       1380 
LVIGICSLLH ERKKTMAKKK GAVLLGLALT STGWFSPTTI AAGLMVCNPN KKRGWPATEF 

      1390       1400       1410       1420       1430       1440 
LSAVGLMFAI VGGLAELDIE SMSIPFMLAG LMAVSYVVSG KATDMWLERA ADISWEMDAA 

      1450       1460       1470       1480       1490       1500 
ITGSSRRLDV KLDDDGDFHL IDDPGVPWKV WVLRMSCIGL AALTPWAIVP AAFGYWLTLK 

      1510       1520       1530       1540       1550       1560 
TTKRGGVFWD TPSPKPCSKG DTTTGVYRIM ARGILGTYQA GVGVMYENVF HTLWHTTRGA 

      1570       1580       1590       1600       1610       1620 
AIMSGEGKLT PYWGSVREDR IAYGGPWRFD RKWNGTDDVQ VIVVEPGKAA VNIQTKPGVF 

      1630       1640       1650       1660       1670       1680 
RTPFGEVGAV SLDYPRGTSG SPILDSNGDI IGLYGNGVEL GDGSYVSAIV QGDRQEEPVP 

      1690       1700       1710       1720       1730       1740 
EAYTPNMLRK RQMTVLDLHP GSGKTRKILP QIIKDAIQQR LRTAVLAPTR VVAAEMAEAL 

      1750       1760       1770       1780       1790       1800 
RGLPVRYQTS AVQREHQGNE IVDVMCHATL THRLMSPNRV PNYNLFVMDE AHFTDPASIA 

      1810       1820       1830       1840       1850       1860 
ARGYIATKVE LGEAAAIFMT ATPPGTTDPF PDSNAPIHDL QDEIPDRAWS SGYEWITEYA 

      1870       1880       1890       1900       1910       1920 
GKTVWFVASV KMGNEIAMCL QRAGKKVIQL NRKSYDTEYP KCKNGDWDFV ITTDISEMGA 

      1930       1940       1950       1960       1970       1980 
NFGASRVIDC RKSVKPTILE EGEGRVILGN PSPITSASAA QRRGRVGRNP NQVGDEYHYG 

      1990       2000       2010       2020       2030       2040 
GATSEDDSNL AHWTEAKIML DNIHMPNGLV AQLYGPEREK AFTMDGEYRL RGEEKKNFLE 

      2050       2060       2070       2080       2090       2100 
LLRTADLPVW LAYKVASNGI QYTDRKWCFD GPRTNAILED NTEVEIVTRM GERKILKPRW 

      2110       2120       2130       2140       2150       2160 
LDARVYADHQ ALKWFKDFAA GKRSAVSFIE VLGRMPEHFM GKTREALDTM YLVATAEKGG 

      2170       2180       2190       2200       2210       2220 
KAHRMALEEL PDALETITLI VAITVMTGGF FLLMMQRKGI GKMGLGALVL TLATFFLWAA 

      2230       2240       2250       2260       2270       2280 
EVPGTKIAGT LLIALLLMVV LIPEPEKQRS QTDNQLAVFL ICVLTVVGVV AANEYGMLEK 

      2290       2300       2310       2320       2330       2340 
TKADLKSMFG GKTQASGLTG LPSMALDLRP ATAWALYGGS TVVLTPLLKH LITSEYVTTS 

      2350       2360       2370       2380       2390       2400 
LASINSQAGS LFVLPRGVPF TDLDLTVGLV FLGCWGQITL TTFLTAMVLA TLHYGYMLPG 

      2410       2420       2430       2440       2450       2460 
WQAEALRAAQ RRTAAGIMKN AVVDGMVATD VPELERTTPL MQKKVGQVLL IGVSVAAFLV 

      2470       2480       2490       2500       2510       2520 
NPNVTTVREA GVLVTAATLT LWDNGASAVW NSTTATGLCH VMRGSYLAGG SIAWTLIKNA 

      2530       2540       2550       2560       2570       2580 
DKPSLKRGRP GGRTLGEQWK EKLNAMSREE FFKYRREAII EVDRTEARRA RRENNIVGGH 

      2590       2600       2610       2620       2630       2640 
PVSRGSAKLR WLVEKGFVSP IGKVIDLGCG RGGWSYYAAT LKKVQEVRGY TKGGAGHEEP 

      2650       2660       2670       2680       2690       2700 
MLMQSYGWNL VSLKSGVDVF YKPSEPSDTL FCDIGESSPS PEVEEQRTLR VLEMTSDWLH 

      2710       2720       2730       2740       2750       2760 
RGPREFCIKV LCPYMPKVIE KMEVLQRRFG GGLVRLPLSR NSNHEMYWVS GAAGNVVHAV 

      2770       2780       2790       2800       2810       2820 
NMTSQVLLGR MDRTVWRGPK YEEDVNLGSG TRAVGKGEVH SNQEKIKKRI QKLKEEFATT 

      2830       2840       2850       2860       2870       2880 
WHKDPEHPYR TWTYHGSYEV KATGSASSLV NGVVELMSKP WDAIANVTTM AMTDTTPFGQ 

      2890       2900       2910       2920       2930       2940 
QRVFKEKVDT KAPEPPAGAK EVLNETTNWL WAHLSREKRP RLCTKEEFIK KVNSNAALGA 

      2950       2960       2970       2980       2990       3000 
VFAEQNQWST AREAVDDPRF WEMVDEEREN HLRGECHTCI YNMMGKREKK PGEFGKAKGS 

      3010       3020       3030       3040       3050       3060 
RAIWFMWLGA RYLEFEALGF LNEDHWLSRE NSGGGVEGSG VQKLGYILRD IAGKQGGKMY 

      3070       3080       3090       3100       3110       3120 
ADDTAGWDTR ITRTDLENEA KVLELLDGEH RMLARAIIEL TYRHKVVKVM RPAAEGKTVM 

      3130       3140       3150       3160       3170       3180 
DVISREDQRG SGQVVTYALN TFTNIAVQLV RLMEAEGVIG PQHLEQLPRK TKIAVRTWLF 

      3190       3200       3210       3220       3230       3240 
ENGEERVTRM AISGDDCVVK PLDDRFATAL HFLNAMSKVR KDIQEWKPSH GWHDWQQVPF 

      3250       3260       3270       3280       3290       3300 
CSNHFQEIVM KDGRSIVVPC RGQDELIGRA RISPGAGWNV KDTACLAKAY AQMWLLLYFH 

      3310       3320       3330       3340       3350       3360 
RRDLRLMANA ICSAVPVDWV PTGRTSWSIH SKGEWMTTED MLQVWNRVWI EENEWMMDKT 

      3370       3380       3390       3400       3410       3420 
PITSWTDVPY VGKREDIWCG SLIGTRSRAT WAENIYAAIN QVRAVIGKEN YVDYMTSLRR 

      3430 
YEDVLIQEDR VI
P27395 in FASTA format

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