ID   3BHS1_MACMU             Reviewed;         373 AA.
AC   P27365;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-NOV-2008, entry version 62.
DE   RecName: Full=3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1;
DE            Short=3-beta-HSD I;
DE   Includes:
DE     RecName: Full=3-beta-hydroxy-Delta(5)-steroid dehydrogenase;
DE              EC=1.1.1.145;
DE     AltName: Full=3-beta-hydroxy-5-ene steroid dehydrogenase;
DE     AltName: Full=Progesterone reductase;
DE   Includes:
DE     RecName: Full=Steroid Delta-isomerase;
DE              EC=5.3.3.1;
DE     AltName: Full=Delta-5-3-ketosteroid isomerase;
GN   Name=HSD3B1;
OS   Macaca mulatta (Rhesus macaque).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
OX   NCBI_TaxID=9544;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Ovary;
RX   MEDLINE=91267292; PubMed=2050270; DOI=10.1016/0303-7207(91)90224-G;
RA   Simard J., Melner M.H., Breton N., Low K.G., Zhao H.-F., Periman L.M.,
RA   Labrie F.;
RT   "Characterization of macaque 3 beta-hydroxy-5-ene steroid
RT   dehydrogenase/delta 5-delta 4 isomerase: structure and expression in
RT   steroidogenic and peripheral tissues in primate.";
RL   Mol. Cell. Endocrinol. 75:101-110(1991).
CC   -!- FUNCTION: 3-beta-HSD is a bifunctional enzyme, that catalyzes the
CC       oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and
CC       the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic
CC       system plays a crucial role in the biosynthesis of all classes of
CC       hormonal steroids.
CC   -!- CATALYTIC ACTIVITY: A 3-beta-hydroxy-Delta(5)-steroid + NAD(+) = a
CC       3-oxo-Delta(5)-steroid + NADH.
CC   -!- CATALYTIC ACTIVITY: A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-
CC       steroid.
CC   -!- PATHWAY: Lipid metabolism; steroid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC       membrane protein. Mitochondrion membrane; Single-pass membrane
CC       protein.
CC   -!- TISSUE SPECIFICITY: Adrenal glands, testes and ovaries.
CC   -!- SIMILARITY: Belongs to the 3-beta-HSD family.
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DR   EMBL; M67467; AAA36847.1; -; mRNA.
DR   PIR; A54325; A54325.
DR   RefSeq; XP_001113717.1; -.
DR   RefSeq; XP_001113744.1; -.
DR   RefSeq; XP_001113769.1; -.
DR   UniGene; Mmu.15109; -.
DR   Ensembl; ENSMMUG00000016568; Macaca mulatta.
DR   GeneID; 712686; -.
DR   HOVERGEN; P27365; -.
DR   LinkHub; P27365; -.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0003854; F:3-beta-hydroxy-delta5-steroid dehydrogenase...; IEA:InterPro.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004769; F:steroid delta-isomerase activity; IEA:EC.
DR   GO; GO:0006700; P:C21-steroid hormone biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002225; 3Beta_OHSteriod_DHase/Estase.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF01073; 3Beta_HSD; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Isomerase; Membrane; Mitochondrion;
KW   Multifunctional enzyme; NAD; Oxidoreductase; Steroidogenesis;
KW   Transmembrane.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    373       3 beta-hydroxysteroid dehydrogenase/Delta
FT                                5-->4-isomerase type 1.
FT                                /FTId=PRO_0000087776.
FT   TRANSMEM    288    308       Potential.
SQ   SEQUENCE   373 AA;  42005 MW;  9B6C172F319B5BB9 CRC64;
     MTGWSCLVTG AGGFLGQRIV RLLVEEKELK EIRVLDKAFR PELREEFSKL QNKTKLTVLE
     GDILDEPFLK RACQDVSVVI HTACIIDVFG VTHRESIMNV NVKGTQLLLE ACVQASVPVF
     IYTSTLEVAG PNSYKEIIQN GHEEEPLENT WPAPYPYSKK LAEKAVLAAN GWTLKNGGTL
     YTCALRPMYI YGEGGPFLSA SINEALNNNG ILSSVGKFST VNPVYVGNVA WAHILALRAL
     RDPKKAPSVQ GQFYYISDDT PHQSYDNLNY ILSKEFGLCL DSRWSLPLAL MYWIGFLLEV
     VSFLLSPVYS YQPPFNRHTV TLSNSVFTFS YKKAQRDLAY KPLYSWEEAK QKTVEWVGSL
     VDRHKETLKS KTQ
//