ID   3BHS_VACCV              Reviewed;         346 AA.
AC   P26670; Q76ZN3;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-NOV-2008, entry version 49.
DE   RecName: Full=3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase;
DE            Short=3-beta-HSD;
DE   Includes:
DE     RecName: Full=3-beta-hydroxy-Delta(5)-steroid dehydrogenase;
DE              EC=1.1.1.145;
DE     AltName: Full=3-beta-hydroxy-5-ene steroid dehydrogenase;
DE     AltName: Full=Progesterone reductase;
DE   Includes:
DE     RecName: Full=Steroid Delta-isomerase;
DE              EC=5.3.3.1;
DE     AltName: Full=Delta-5-3-ketosteroid isomerase;
GN   OrderedLocusNames=VACWR170; ORFNames=SALF7L;
OS   Vaccinia virus (strain Western Reserve / WR) (VACV).
OC   Viruses; dsDNA viruses, no RNA stage; Poxviridae; Chordopoxvirinae;
OC   Orthopoxvirus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=91332999; PubMed=1870190;
RA   Blasco R., Cole N.B., Moss B.;
RT   "Sequence analysis, expression, and deletion of a vaccinia virus gene
RT   encoding a homolog of profilin, a eukaryotic actin-binding protein.";
RL   J. Virol. 65:4598-4608(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=91259063; PubMed=2045793;
RA   Smith G.L., Chan Y.S., Howard S.T.;
RT   "Nucleotide sequence of 42 kbp of vaccinia virus strain WR from near
RT   the right inverted terminal repeat.";
RL   J. Gen. Virol. 72:1349-1376(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M.,
RA   Osborne J., Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   FUNCTION.
RX   MEDLINE=92258409; PubMed=1582424;
RA   Moore J.B., Smith G.L.;
RT   "Steroid hormone synthesis by a vaccinia enzyme: a new type of virus
RT   virulence factor.";
RL   EMBO J. 11:1973-1980(1992).
CC   -!- FUNCTION: 3-beta-HSD is a bifunctional enzyme, that catalyzes the
CC       oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and
CC       the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic
CC       system plays a crucial role in the biosynthesis of all classes of
CC       hormonal steroids.
CC   -!- CATALYTIC ACTIVITY: A 3-beta-hydroxy-Delta(5)-steroid + NAD(+) = a
CC       3-oxo-Delta(5)-steroid + NADH.
CC   -!- CATALYTIC ACTIVITY: A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-
CC       steroid.
CC   -!- PATHWAY: Lipid metabolism; steroid biosynthesis.
CC   -!- SIMILARITY: Belongs to the 3-beta-HSD family.
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DR   EMBL; M72474; AAA48311.1; -; Genomic_DNA.
DR   EMBL; D11079; BAA01818.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89449.1; -; Genomic_DNA.
DR   PIR; E40897; WMVZ2W.
DR   RefSeq; YP_233052.1; -.
DR   GeneID; 3707700; -.
DR   GO; GO:0003854; F:3-beta-hydroxy-delta5-steroid dehydrogenase...; IEA:InterPro.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004769; F:steroid delta-isomerase activity; IEA:EC.
DR   GO; GO:0006700; P:C21-steroid hormone biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002225; 3Beta_OHSteriod_DHase/Estase.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF01073; 3Beta_HSD; 1.
PE   3: Inferred from homology;
KW   Isomerase; Multifunctional enzyme; NAD; Oxidoreductase;
KW   Steroidogenesis.
FT   CHAIN         1    346       3 beta-hydroxysteroid dehydrogenase/Delta
FT                                5-->4-isomerase.
FT                                /FTId=PRO_0000087795.
SQ   SEQUENCE   346 AA;  39396 MW;  3107A2B7F442CCD9 CRC64;
     MAVYAVTGGA GFLGRYIVKL LISADDVQEI RVIDIVEDPQ PITSKVKVIN YIQCDINDFD
     KVREALDGVN LIIHTAALVD VFGKYTDNEI MKVNYYGTQT ILAACVDLGI KYLIYTSSME
     AIGPNKHGDP FIGHEHTLYD ISPGHVYAKS KRMAEQLVMK ANNSVIMNGA KLYTCCLRPT
     GIYGEGDKLT KVFYEQCKQH GNIMYRTVDD DAVHSRVYVG NVAWMHVLAA KYIQYPGSEI
     KGNAYFCYDY SPSCSYDMFN LLLMKPLGIE QGSRIPRWML KMYACKNDMK RILFRKPSLL
     NNYTLKISNT TFEVRTNNAE LDFNYSPIFN VDVAFERTRK WLEESE
//