3-beta-hydroxy-Delta(5)-steroid dehydrogenase
     (EC 1.1.1.145)
     (3-beta-hydroxy-5-ene steroid dehydrogenase)
     (Progesterone reductase)
Steroid Delta-isomerase
     (EC 5.3.3.1)
     (Delta-5-3-ketosteroid isomerase)

Gene name

	OrderedLocusNames:	VACWR170
	ORFNames:	SALF7L

From

Vaccinia virus (strain Western Reserve / WR) (VACV)

[TaxID: 10254]

Taxonomy

Viruses; dsDNA viruses, no RNA stage; Poxviridae; Chordopoxvirinae; Orthopoxvirus.

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein existence

3: Inferred from homology;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=1870190 [NCBI, ExPASy, EBI, Israel, Japan] Blasco R., Cole N.B., Moss B.; "Sequence analysis, expression, and deletion of a vaccinia virus gene encoding a homolog of profilin, a eukaryotic actin-binding protein."; J. Virol. 65:4598-4608(1991).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=2045793 [NCBI, ExPASy, EBI, Israel, Japan] Smith G.L., Chan Y.S., Howard S.T.; "Nucleotide sequence of 42 kbp of vaccinia virus strain WR from near the right inverted terminal repeat."; J. Gen. Virol. 72:1349-1376(1991).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.; "Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."; Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
[4]	FUNCTION. PubMed=1582424 [NCBI, ExPASy, EBI, Israel, Japan] Moore J.B., Smith G.L.; "Steroid hormone synthesis by a vaccinia enzyme: a new type of virus virulence factor."; EMBO J. 11:1973-1980(1992).

Comments

FUNCTION: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
CATALYTIC ACTIVITY: A 3-beta-hydroxy-Delta⁵-steroid + NAD⁺ = a 3-oxo-Delta⁵-steroid + NADH.
CATALYTIC ACTIVITY: A 3-oxo-Delta⁵-steroid = a 3-oxo-Delta⁴-steroid.
PATHWAY: Lipid metabolism; steroid biosynthesis .
SIMILARITY: Belongs to the 3-beta-HSD family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M72474; AAA48311.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
D11079; BAA01818.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY243312; AAO89449.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

E40897; WMVZ2W.

3D structure databases

ModBase

P26670.

Ontologies

GO:0004769;	Molecular function: steroid delta-isomerase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR002225; 3Beta_OHSteriod_DHase/Estase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

Pfam

PF01073; 3Beta_HSD; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Isomerase; Multifunctional enzyme; NAD; Oxidoreductase; Steroidogenesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 346`	`346`		`3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase.`	`PRO_0000087795`

Sequence information

Length: 346 AA [This is the length of the unprocessed precursor]

Molecular weight: 39396 Da [This is the MW of the unprocessed precursor]

CRC64: 3107A2B7F442CCD9 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAVYAVTGGA GFLGRYIVKL LISADDVQEI RVIDIVEDPQ PITSKVKVIN YIQCDINDFD 

        70         80         90        100        110        120 
KVREALDGVN LIIHTAALVD VFGKYTDNEI MKVNYYGTQT ILAACVDLGI KYLIYTSSME 

       130        140        150        160        170        180 
AIGPNKHGDP FIGHEHTLYD ISPGHVYAKS KRMAEQLVMK ANNSVIMNGA KLYTCCLRPT 

       190        200        210        220        230        240 
GIYGEGDKLT KVFYEQCKQH GNIMYRTVDD DAVHSRVYVG NVAWMHVLAA KYIQYPGSEI 

       250        260        270        280        290        300 
KGNAYFCYDY SPSCSYDMFN LLLMKPLGIE QGSRIPRWML KMYACKNDMK RILFRKPSLL 

       310        320        330        340 
NNYTLKISNT TFEVRTNNAE LDFNYSPIFN VDVAFERTRK WLEESE

P26670 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools