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UniProtKB/Swiss-Prot entry P25995


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PYRC_BACSU
Primary accession number P25995
Secondary accession numbers None
Integrated into Swiss-Prot on May 1, 1992
Sequence was last modified on May 1, 1992 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 75)
Name and origin of the protein
Protein name Dihydroorotase
Synonyms DHOase
EC 3.5.2.3
Gene name
Name: pyrC
OrderedLocusNames: BSU15500
From
Bacillus subtilis [TaxID: 1423] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 3: Inferred from homology;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=1709162 [NCBI, ExPASy, EBI, Israel, Japan]
Quinn C.L., Stephenson B.T., Switzer R.L.;
"Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon.";
J. Biol. Chem. 266:9113-9127(1991).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=168;
DOI=10.1038/36786; PubMed=9384377 [NCBI, ExPASy, EBI, Israel, Japan]
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.;
"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.";
Nature 390:249-256(1997).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M59757; AAA21268.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z99112; CAB13424.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR D39845; DEBSO.
RefSeq NP_389433.1; -.
3D structure databases
ModBase P25995.
Protein family/group databases
MEROPS M38.972; -.
Enzyme and pathway databases
BioCyc BSUB224308:BSU1552-MON; -.
Organism-specific databases
SubtiList BG10714; pyrC. [Micado]
Ontologies
GO
GO:0004151; Molecular function: dihydroorotase activity (inferred from electronic annotation from HAMAP).
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from HAMAP).
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.
Family and domain databases
HAMAP MF_00220; -; 1.
PBIL [Tree]
InterPro IPR006680; Amidohydro_1.
IPR004722; DHOmult.
IPR002195; Dihydroorotase_CS.
Graphical view of domain structure.
Pfam PF01979; Amidohydro_1; 1.
Pfam graphical view of domain structure.
ProDom PD000518; DHOase; 1.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR00857; pyrC_multi; 1.
PROSITE PS00482; DIHYDROOROTASE_1; 1.
PS00483; DIHYDROOROTASE_2; 1.
BLOCKS P25995.
ProtoNet P25995.
Genome annotation databases
GeneID 937095; -.
GenomeReviews AL009126_GR; BSU15500.
KEGG bsu:BSU15500; -.
NMPDR fig|224308.1.peg.1552; -.
Phylogenomic databases
HOGENOM P25995; -.
Genome annotation databases
CMR P25995; BSU15500.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Hydrolase; Metal-binding; Pyrimidine biosynthesis; Zinc.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   428  428     Dihydroorotase. PRO_0000147229
METAL   59    59        Zinc 1 (By similarity). 
METAL   61    61        Zinc 1 (By similarity). 
METAL   141   141        Zinc 1; via carbamate group (By similarity). 
METAL   141   141        Zinc 2; via carbamate group (By similarity). 
METAL   178   178        Zinc 2 (By similarity). 
METAL   230   230        Zinc 2 (By similarity). 
METAL   303   303        Zinc 1 (By similarity). 
MOD_RES   141   141        N6-carboxylysine (By similarity). 
Sequence information
Length: 428 AA [This is the length of the unprocessed precursor] Molecular weight: 46609 Da [This is the MW of the unprocessed precursor] CRC64: 0DC49B0F37DE0E2B [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSYLIKNGWI LNENGEKTQA DIRVTGETIT AIGKLDATDN ETVIDAKGLL VSPGFVDLHV 

        70         80         90        100        110        120 
HFREPGGEKK ETIETGAKAA GRGGYTTVAA MPNTRPVPDT KEQMEWVQNR IKETSCVRVL 

       130        140        150        160        170        180 
PYASITIRQI GDEMTNFEAL KEAGAFAFTD DGVGIQTAGM MYEAMKRAAA IDKAIVAHCE 

       190        200        210        220        230        240 
DNSLIYGGSV HEGTFSKANG LNGIPSVCES VHIARDVLLL GANCHYHVCH ISTKESVRVV 

       250        260        270        280        290        300 
RDAKKAGIRV TAEVSPHHLL LCDEDIPGLD TNYKMNPPLR SPEDRAALIE GLLDGTIDFI 

       310        320        330        340        350        360 
ATDHAPHTEE EKNTEMKLAP FRNCRLRNSI PASLHTLCQK WQLVTEAADL TYMTIKPCEA 

       370        380        390        400        410        420 
FGLPYGTLQT GQAADITLID LEKEAVIDKE TFLSKGKNTP FNGISCTGWP VATIAAGKLA 


YEEGRLVK 

P25995 in FASTA format

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