ID   MCEL_RFVKA              Reviewed;         836 AA.
AC   P25950;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   25-NOV-2008, entry version 46.
DE   RecName: Full=mRNA-capping enzyme large subunit;
DE   Includes:
DE     RecName: Full=Polynucleotide 5'-triphosphatase;
DE              EC=3.1.3.33;
DE     AltName: Full=mRNA 5'-triphosphatase;
DE              Short=TPase;
DE   Includes:
DE     RecName: Full=mRNA guanylyltransferase;
DE              EC=2.7.7.50;
DE     AltName: Full=GTP--RNA guanylyltransferase;
DE              Short=GTase;
GN   ORFNames=D3R;
OS   Rabbit fibroma virus (strain Kasza) (RFV) (Shope fibroma virus (strain
OS   Kasza)).
OC   Viruses; dsDNA viruses, no RNA stage; Poxviridae; Chordopoxvirinae;
OC   Leporipoxvirus.
OX   NCBI_TaxID=10272;
OH   NCBI_TaxID=9986; Oryctolagus cuniculus (Rabbit).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=91306463; PubMed=1649507; DOI=10.1016/0042-6822(91)91009-6;
RA   Upton C., Stuart D., McFadden G.;
RT   "Identification and DNA sequence of the large subunit of the capping
RT   enzyme from Shope fibroma virus.";
RL   Virology 183:773-777(1991).
CC   -!- FUNCTION: Catalyzes the first two reactions in the mRNA cap
CC       formation pathway.
CC   -!- CATALYTIC ACTIVITY: A 5'-phosphopolynucleotide + H(2)O = a
CC       polynucleotide + phosphate.
CC   -!- CATALYTIC ACTIVITY: GTP + (5')pp-Pur-mRNA = diphosphate +
CC       G(5')ppp-Pur-mRNA.
CC   -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC   -!- SIMILARITY: Belongs to the viral GTase family.
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DR   EMBL; M63902; AAA47224.1; -; Genomic_DNA.
DR   PIR; A40478; QQVZRA.
DR   GO; GO:0004484; F:mRNA guanylyltransferase activity; IEA:EC.
DR   GO; GO:0004651; F:polynucleotide 5'-phosphatase activity; IEA:EC.
DR   GO; GO:0006370; P:mRNA capping; IEA:InterPro.
DR   InterPro; IPR004971; Pox_MCEL.
DR   InterPro; IPR002035; VWF_A.
DR   Pfam; PF03291; Pox_MCEL; 1.
DR   PRINTS; PR00453; VWFADOMAIN.
PE   3: Inferred from homology;
KW   Hydrolase; mRNA capping; mRNA processing; Multifunctional enzyme;
KW   Nucleotidyltransferase; Transferase.
FT   CHAIN         1    836       mRNA-capping enzyme large subunit.
FT                                /FTId=PRO_0000210131.
FT   ACT_SITE    256    256       N6-GMP-lysine intermediate (Potential).
SQ   SEQUENCE   836 AA;  97018 MW;  7375FDA548AE1730 CRC64;
     MDDSKKKRGT DYIEELILLY EDVPNPVQTD DMNHEVELTF IQPPVITLST LLPFATSQES
     YILFTVTNKG VKIRNRINLS KIHGLDLKNI QLVDSIDNII WEKKTLVKEH KIDSVALVKY
     STEEKYIFLD YKKYLSAIKL ELVNVVQVKV KHVTVDFKFK YFLGSGAQAK SSLLHVLNHP
     KSKPNPSLEF EIITTDEKID SASLRKELIA LFKLVFMASP SNIILDVVFK NPVQTILLKK
     NELPGIDLTN LYVTTKTDGV GVLITVTNKG IYCFFTHLQY TIRYDTTFES NESVTLYGEA
     VKQNNVWQIY LIKLITPKVS DRFKEKEYVE ERLQNICDRM TFKVKKYEGP FESHSEIIDL
     LTTYLPSQPE GVVLFYSDQR NQPDYKIKLD NTTDHMINII YRYMSSEPVI FGENSTFLEY
     KKFSDDKGFP KDYGTGKLML TDNVRYLNNI YCIAFTNVYE DVGIKNVVVP IKFISEFSAT
     GELIKPRIDK TFKYLYKEYY GNQYQIVVAH IRDQNIKIGD VLDEDKLSDV GQHYANDKYR
     LNPDVSYFTN KRTRGPLGIL SNYVKTLLIS LYCSKTFLDN SNKRKVLAID FGNGADLEKY
     FYGEISSLVA TDPDKEAIGR CIERYNSLNS GIKSKYYKFD YIQETIRSVT YVSSVREVFF
     FGKFDLVDWQ FAIHYSFHPK HYATVMNNLT ELTASGGKVL ITTMDGDLLS QLTDKKTFVI
     HKNLPSSENY MSVEKIHEDQ ILVYNPSSMS RPMQEYIVKR VNLTKIFSEY GFELIDCVHF
     DTIIERSKRF INSVSKMEER KSTKNFFELN REALKHEGTD IDDLLRYYIV YVFSKR
//