ID   FLIF_ECOLI              Reviewed;         552 AA.
AC   P25798; P76324; P76914;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 3.
DT   25-NOV-2008, entry version 69.
DE   RecName: Full=Flagellar M-ring protein;
GN   Name=fliF; Synonyms=fla AII.1, fla BI;
GN   OrderedLocusNames=b1938, JW1922;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA   al Mamun A.A.M., Tominaga A., Enomoto M.;
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   MEDLINE=97251358; PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA   Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA   Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA   Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA   Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA   Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C.,
RA   Yamamoto Y., Horiuchi T.;
RT   "A 460-kb DNA sequence of the Escherichia coli K-12 genome
RT   corresponding to the 40.1-50.0 min region on the linkage map.";
RL   DNA Res. 3:379-392(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1474(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5.
RC   STRAIN=JA11;
RX   MEDLINE=92202159; PubMed=1551848;
RA   Mueller V., Jones C.J., Kawagishi I., Aizawa S., Macnab R.M.;
RT   "Characterization of the fliE genes of Escherichia coli and Salmonella
RT   typhimurium and identification of the FliE protein as a component of
RT   the flagellar hook-basal body complex.";
RL   J. Bacteriol. 174:2298-2304(1992).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 532-552.
RX   MEDLINE=94040782; PubMed=8224881; DOI=10.1016/0378-1119(93)90232-R;
RA   Roman S.J., Frantz B.B., Matsumura P.;
RT   "Gene sequence, overproduction, purification and determination of the
RT   wild-type level of the Escherichia coli flagellar switch protein
RT   FliG.";
RL   Gene 133:103-108(1993).
RN   [7]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- FUNCTION: The M ring may be actively involved in energy
CC       transduction.
CC   -!- SUBUNIT: The basal body constitutes a major portion of the
CC       flagellar organelle and consists of four rings (L,P,S, and M)
CC       mounted on a central rod. The M ring is integral to the inner
CC       membrane of the cell and may be connected to the flagellar rod via
CC       the S ring. The S (supramembrane ring) lies just distal to the M
CC       ring. The L and P rings lie in the outer membrane and the
CC       periplasmic space, respectively.
CC   -!- INTERACTION:
CC       P0AC41:sdhA; NbExp=1; IntAct=EBI-1126492, EBI-371263;
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane
CC       protein. Bacterial flagellum basal body.
CC   -!- SIMILARITY: Belongs to the fliF family.
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DR   EMBL; D89826; BAA14029.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75005.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15763.1; -; Genomic_DNA.
DR   EMBL; M84992; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; L13243; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; G64957; G64957.
DR   RefSeq; AP_002550.1; -.
DR   RefSeq; NP_416448.1; -.
DR   DIP; DIP:401N; -.
DR   IntAct; P25798; -.
DR   GeneID; 946448; -.
DR   GenomeReviews; U00096_GR; b1938.
DR   GenomeReviews; AP009048_GR; JW1922.
DR   KEGG; ecj:JW1922; -.
DR   KEGG; eco:b1938; -.
DR   EchoBASE; EB1323; -.
DR   EcoGene; EG11347; fliF.
DR   HOGENOM; P25798; -.
DR   BioCyc; EcoCyc:FLIF-FLAGELLAR-MS-RING; -.
DR   GO; GO:0009431; C:flagellin-based flagellum basal body, MS ring; IEA:InterPro.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0003774; F:motor activity; IEA:InterPro.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0001539; P:ciliary or flagellar motility; IEA:InterPro.
DR   InterPro; IPR013556; Flag_M-ring_C.
DR   InterPro; IPR000067; FlgMring_FLIF.
DR   InterPro; IPR006182; YscJ_FliF.
DR   Pfam; PF01514; YscJ_FliF; 1.
DR   Pfam; PF08345; YscJ_FliF_C; 1.
DR   PRINTS; PR01009; FLGMRINGFLIF.
DR   TIGRFAMs; TIGR00206; fliF; 1.
PE   1: Evidence at protein level;
KW   Bacterial flagellum; Cell inner membrane; Cell membrane;
KW   Complete proteome; Membrane; Transmembrane.
FT   CHAIN         1    552       Flagellar M-ring protein.
FT                                /FTId=PRO_0000180882.
FT   TRANSMEM     23     43       Potential.
FT   TRANSMEM    447    467       Potential.
FT   CONFLICT    260    260       H -> R (in Ref. 1; BAA14029).
FT   CONFLICT    266    266       Q -> H (in Ref. 1; BAA14029).
FT   CONFLICT    541    541       L -> V (in Ref. 1).
SQ   SEQUENCE   552 AA;  60589 MW;  B2038FDBEA914DA5 CRC64;
     MNATAAQTKS LEWLNRLRAN PKIPLIVAGS AAVAVMVALI LWAKAPDYRT LFSNLSDQDG
     GAIVSQLTQM NIPYRFSEAS GAIEVPADKV HELRLRLAQQ GLPKGGAVGF ELLDQEKFGI
     SQFSEQVNYQ RALEGELSRT IETIGPVKGA RVHLAMPKPS LFVREQKSPS ASVTVNLLPG
     RALDEGQISA IVHLVSSAVA GLPPGNVTLV DQGGHLLTQS NTSGRDLNDA QLKYASDVEG
     RIQRRIEAIL SPIVGNGNIH AQVTAQLDFA SKEQTEEQYR PNGDESHAAL RSRQLNESEQ
     SGSGYPGGVP GALSNQPAPA NNAPISTPPA NQNNRQQQAS TTSNSGPRST QRNETSNYEV
     DRTIRHTKMN VGDVQRLSVA VVVNYKTLPD GKPLPLSNEQ MKQIEDLTRE AMGFSEKRGD
     SLNVVNSPFN SSDESGGELP FWQQQAFIDQ LLAAGRWLLV LLVAWLLWRK AVRPQLTRRA
     EAMKAVQQQA QAREEVEDAV EVRLSKDEQL QQRRANQRLG AEVMSQRIRE MSDNDPRVVA
     LVIRQWINND HE
//