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UniProtKB/Swiss-Prot entry P25798


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name FLIF_ECOLI
Primary accession number P25798
Secondary accession numbers P76324 P76914
Integrated into Swiss-Prot on May 1, 1992
Sequence was last modified on November 1, 1997 (Sequence version 3)
Annotations were last modified on    July 22, 2008 (Entry version 68)
Name and origin of the protein
Protein name Flagellar M-ring protein
Synonyms None
Gene name
Name: fliF
Synonyms: fla AII.1, fla BI
OrderedLocusNames: b1938, JW1922
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
al Mamun A.A.M., Tominaga A., Enomoto M.;
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1093/dnares/3.6.379; PubMed=9097040 [NCBI, ExPASy, EBI, Israel, Japan]
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.;
"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.";
DNA Res. 3:379-392(1996).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5.
STRAIN=JA11;
PubMed=1551848 [NCBI, ExPASy, EBI, Israel, Japan]
Mueller V., Jones C.J., Kawagishi I., Aizawa S., Macnab R.M.;
"Characterization of the fliE genes of Escherichia coli and Salmonella typhimurium and identification of the FliE protein as a component of the flagellar hook-basal body complex.";
J. Bacteriol. 174:2298-2304(1992).
[6]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 532-552.
DOI=10.1016/0378-1119(93)90232-R; PubMed=8224881 [NCBI, ExPASy, EBI, Israel, Japan]
Roman S.J., Frantz B.B., Matsumura P.;
"Gene sequence, overproduction, purification and determination of the wild-type level of the Escherichia coli flagellar switch protein FliG.";
Gene 133:103-108(1993).
[7]
SUBCELLULAR LOCATION.
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.1109730; PubMed=15919996 [NCBI, ExPASy, EBI, Israel, Japan]
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
"Global topology analysis of the Escherichia coli inner membrane proteome.";
Science 308:1321-1323(2005).
Comments
  • FUNCTION: The M ring may be actively involved in energy transduction.
  • SUBUNIT: The basal body constitutes a major portion of the flagellar organelle and consists of four rings (L,P,S, and M) mounted on a central rod. The M ring is integral to the inner membrane of the cell and may be connected to the flagellar rod via the S ring. The S (supramembrane ring) lies just distal to the M ring. The L and P rings lie in the outer membrane and the periplasmic space, respectively.
  • INTERACTION:
    P0AC41:sdhA; NbExp=1; IntAct=EBI-1126492, EBI-371263;
  • SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body.
  • SIMILARITY: Belongs to the fliF family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
D89826; BAA14029.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC75005.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAA15763.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M84992; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
L13243; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
PIR G64957; G64957.
RefSeq AP_002550.1; -.
NP_416448.1; -.
3D structure databases
ModBase P25798.
Protein-protein interaction databases
DIP DIP:401N; -.
IntAct P25798; -.
Enzyme and pathway databases
BioCyc EcoCyc:FLIF-FLAGELLAR-MS-RING; -.
Organism-specific databases
EchoBASE EB1323; -.
EcoGene EG11347; fliF.
Ontologies
GO
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR013556; Flag_M-ring_C.
IPR000067; FlgMring_FLIF.
IPR006182; YscJ_FliF.
Graphical view of domain structure.
Pfam PF01514; YscJ_FliF; 1.
PF08345; YscJ_FliF_C; 1.
Pfam graphical view of domain structure.
PRINTS PR01009; FLGMRINGFLIF.
TIGRFAMs TIGR00206; fliF; 1.
BLOCKS P25798.
Genome annotation databases
GeneID 946448; -.
GenomeReviews U00096_GR; b1938.
AP009048_GR; JW1922.
KEGG ecj:JW1922; -.
eco:b1938; -.
Phylogenomic databases
HOGENOM P25798; -.
Genome annotation databases
CMR P25798; b1938.
Other
ProtoNet P25798.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Bacterial flagellum; Cell inner membrane; Cell membrane; Complete proteome; Membrane; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   552  552     Flagellar M-ring protein. PRO_0000180882
TRANSMEM   23    43  21     Potential. 
TRANSMEM   447   467  21     Potential. 
CONFLICT   260   260        H -> R (in Ref. 1; BAA14029). 
CONFLICT   266   266        Q -> H (in Ref. 1; BAA14029). 
CONFLICT   541   541        L -> V (in Ref. 1). 
Sequence information
Length: 552 AA [This is the length of the unprocessed precursor] Molecular weight: 60589 Da [This is the MW of the unprocessed precursor] CRC64: B2038FDBEA914DA5 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MNATAAQTKS LEWLNRLRAN PKIPLIVAGS AAVAVMVALI LWAKAPDYRT LFSNLSDQDG 

        70         80         90        100        110        120 
GAIVSQLTQM NIPYRFSEAS GAIEVPADKV HELRLRLAQQ GLPKGGAVGF ELLDQEKFGI 

       130        140        150        160        170        180 
SQFSEQVNYQ RALEGELSRT IETIGPVKGA RVHLAMPKPS LFVREQKSPS ASVTVNLLPG 

       190        200        210        220        230        240 
RALDEGQISA IVHLVSSAVA GLPPGNVTLV DQGGHLLTQS NTSGRDLNDA QLKYASDVEG 

       250        260        270        280        290        300 
RIQRRIEAIL SPIVGNGNIH AQVTAQLDFA SKEQTEEQYR PNGDESHAAL RSRQLNESEQ 

       310        320        330        340        350        360 
SGSGYPGGVP GALSNQPAPA NNAPISTPPA NQNNRQQQAS TTSNSGPRST QRNETSNYEV 

       370        380        390        400        410        420 
DRTIRHTKMN VGDVQRLSVA VVVNYKTLPD GKPLPLSNEQ MKQIEDLTRE AMGFSEKRGD 

       430        440        450        460        470        480 
SLNVVNSPFN SSDESGGELP FWQQQAFIDQ LLAAGRWLLV LLVAWLLWRK AVRPQLTRRA 

       490        500        510        520        530        540 
EAMKAVQQQA QAREEVEDAV EVRLSKDEQL QQRRANQRLG AEVMSQRIRE MSDNDPRVVA 

       550 
LVIRQWINND HE 

P25798 in FASTA format

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