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UniProtKB/Swiss-Prot entry P25765


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CHI2_ORYSJ
Primary accession number P25765
Secondary accession number Q7Y1K8
Integrated into Swiss-Prot on May 1, 1992
Sequence was last modified on September 5, 2006 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 68)
Name and origin of the protein
Protein name Basic endochitinase 2 [Precursor]
Synonym EC 3.2.1.14
Gene name
Name: RCH10
OrderedLocusNames: Os03g0418000, LOC_Os03g30470
ORFNames: OSJNBb0028K20.4, OSJNBb0056B16.17
From
Oryza sativa subsp. japonica (Rice) [TaxID: 39947] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1007/BF00273615; PubMed=2034221 [NCBI, ExPASy, EBI, Israel, Japan]
Zhu Q., Lamb C.J.;
"Isolation and characterization of a rice gene encoding a basic chitinase.";
Mol. Gen. Genet. 226:289-296(1991).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Nipponbare;
DOI=10.1101/gr.3869505; PubMed=16109971 [NCBI, ExPASy, EBI, Israel, Japan]
The rice chromosome 3 sequencing consortium;
Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H.-I., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.;
"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species.";
Genome Res. 15:1284-1291(2005).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AC137992; AAP44624.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC145386; AAR01697.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
DP000009; ABF96639.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S15997; S15997.
3D structure databases
HSSP P23951; 1CNS. [HSSP ENTRY / PDB]
SMR P25765; 22-324.
ModBase P25765.
Organism-specific databases
Gramene P25765; -.
Ontologies
GO
GO:0016231; Molecular function: beta-N-acetylglucosaminidase activity (inferred from direct assay from Gramene).
GO:0008061; Molecular function: chitin binding (inferred from electronic annotation from InterPro).
GO:0008843; Molecular function: endochitinase activity (inferred from direct assay from Gramene).
GO:0016998; Biological process: cell wall catabolic process (inferred from electronic annotation from InterPro).
GO:0006032; Biological process: chitin catabolic process (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR001002; Chitin_bd_1.
IPR016283; Glyco_hydro_19.
IPR000726; Glyco_hydro_19_cat.
Graphical view of domain structure.
Gene3D G3DSA:3.30.60.10; Chitin_bd_1; 1.
PANTHER PTHR22595; Glyco_hydro_19_cat; 1.
Pfam PF00187; Chitin_bind_1; 1.
PF00182; Glyco_hydro_19; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF001060; Endochitinase; 1.
PRINTS PR00451; CHITINBINDNG.
ProDom PD000609; Chitin_binding_1; 1.
PD354900; Glyco_hydro_19; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00270; ChtBD1; 1.
SMART graphical view of domain structure.
PROSITE PS00026; CHIT_BIND_I_1; 1.
PS50941; CHIT_BIND_I_2; 1.
PS00773; CHITINASE_19_1; 1.
PS00774; CHITINASE_19_2; 1.
PROSITE graphical view of domain structure (profiles).
ProtoNet P25765.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Carbohydrate metabolism; Chitin degradation; Chitin-binding; Glycosidase; Hydrolase; Plant defense; Polysaccharide degradation; Signal.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    21  21     Potential. 
CHAIN   22   326  305     Basic endochitinase 2. PRO_0000005306
DOMAIN   22    62  41     Chitin-binding type-1. 
DISULFID   24    39        By similarity. 
DISULFID   33    45        By similarity. 
DISULFID   38    52        By similarity. 
DISULFID   56    60        By similarity. 
DISULFID   102   165        By similarity. 
DISULFID   179   187        By similarity. 
DISULFID   286   318        By similarity. 
Sequence information
Length: 326 AA [This is the length of the unprocessed precursor] Molecular weight: 33636 Da [This is the MW of the unprocessed precursor] CRC64: 41E748706FAFEDC1 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MRALAVVAMV ATAFLAAAVH AEQCGSQAGG AVCPNCLCCS QFGWCGSTSD YCGAGCQSQC 

        70         80         90        100        110        120 
SAAGCGGGGP TPPSGSGGSG VASIVSRSLF DQMLLHRNDA ACPASNFYTY DAFVAAASAF 

       130        140        150        160        170        180 
PGFAAAGGDA DTNKREVAAF LAQTSHETTG GWATAPDGPY AWGYCFKEEN GGAAGPDYCQ 

       190        200        210        220        230        240 
QSAQWPCAAG KKYYGRGPIQ LSYNFNYGPA GQAIGADLLG DPDLVASDAT VSFDTAFWFW 

       250        260        270        280        290        300 
MTPQSPKPSC HAVATGQWTP SADDQAAGRV PGYGVITNII NGGLECGHGE DDRVADRIGF 

       310        320 
YKRYCDILGV SYDANLDCYS QRPFGS 

P25765 in FASTA format

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BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

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