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UniProtKB/Swiss-Prot entry P25524


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CODA_ECOLI
Primary accession number P25524
Secondary accession number Q2MC87
Integrated into Swiss-Prot on May 1, 1992
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    November 25, 2008 (Entry version 79)
Name and origin of the protein
Protein name Cytosine deaminase
Synonyms EC 3.5.4.1
Cytosine aminohydrolase
Gene name
Name: codA
OrderedLocusNames: b0337, JW0328
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-20.
STRAIN=CSH01;
PubMed=1640834 [NCBI, ExPASy, EBI, Israel, Japan]
Danielsen S., Kilstrup M., Barilla K., Jochimsen B., Neuhard J.;
"Characterization of the Escherichia coli codBA operon encoding cytosine permease and cytosine deaminase.";
Mol. Microbiol. 6:1335-1344(1992).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=8450832 [NCBI, ExPASy, EBI, Israel, Japan]
Austin E.A., Huber B.E.;
"A first step in the development of gene therapy for colorectal carcinoma: cloning, sequencing, and expression of Escherichia coli cytosine deaminase.";
Mol. Pharmacol. 43:380-387(1993).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
"Sequence of minutes 4-25 of Escherichia coli.";
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[6]
X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS).
DOI=10.1006/jmbi.2001.5277; PubMed=11812140 [NCBI, ExPASy, EBI, Israel, Japan]
Ireton G.C., McDermott G., Black M.E., Stoddard B.L.;
"The structure of Escherichia coli cytosine deaminase.";
J. Mol. Biol. 315:687-697(2002).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X63656; CAA45196.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S56903; AAB25761.2; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U73857; AAB18061.1; ALT_INIT; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC73440.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAE76119.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S22662; S22662.
RefSeq AP_000989.1; -.
NP_414871.1; -.
3D structure databases
PDB
1K6W; X-ray; 1.75 A; A=1-427.[ExPASy / RCSB / EBI]
1K70; X-ray; 1.80 A; A=1-427.[ExPASy / RCSB / EBI]
1R9X; X-ray; 1.58 A; A=3-427.[ExPASy / RCSB / EBI]
1R9Y; X-ray; 1.57 A; A=3-427.[ExPASy / RCSB / EBI]
1R9Z; X-ray; 1.32 A; A=3-427.[ExPASy / RCSB / EBI]
1RA0; X-ray; 1.12 A; A=3-427.[ExPASy / RCSB / EBI]
1RA5; X-ray; 1.40 A; A=3-427.[ExPASy / RCSB / EBI]
1RAK; X-ray; 1.32 A; A=3-427.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1K6W; -.
1K70; -.
1R9X; -.
1R9Y; -.
1R9Z; -.
1RA0; -.
1RA5; -.
1RAK; -.
ModBase P25524.
Protein-protein interaction databases
DIP DIP:9306N; -.
Enzyme and pathway databases
BioCyc EcoCyc:CYTDEAM-MON; -.
MetaCyc:CYTDEAM-MON; -.
Organism-specific databases
EchoBASE EB1302; -.
EcoGene EG11326; codA.
Ontologies
GO
GO:0005829; Cellular component: cytosol (inferred from direct assay from UniProtKB).
GO:0004131; Molecular function: cytosine deaminase activity (inferred from electronic annotation from EC).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0019858; Biological process: cytosine metabolic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR013108; Amidohydro_3.
Graphical view of domain structure.
Pfam PF07969; Amidohydro_3; 1.
Pfam graphical view of domain structure.
ProtoNet P25524.
Genome annotation databases
GeneID 944996; -.
GenomeReviews U00096_GR; b0337.
AP009048_GR; JW0328.
KEGG ecj:JW0328; -.
eco:b0337; -.
Phylogenomic databases
HOGENOM P25524; -.
Other
LinkHub P25524; -.
Genome annotation databases
CMR P25524; b0337.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Complete proteome; Cytosine metabolism; Direct protein sequencing; Hydrolase; Iron; Metal-binding.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   427  426     Cytosine deaminase. PRO_0000090002
METAL   62    62        Iron. 
METAL   64    64        Iron. 
VARIANT   13    13  1     R -> W (in strain: SO5076). 
STRAND   8    13  6      
STRAND   19    26  8      
STRAND   29    38  10      
STRAND   46    48  3      
STRAND   53    56  4      
STRAND   58    63  6      
TURN   65    69  5      
STRAND   73    75  3      
HELIX   82    90  9      
HELIX   91    95  5      
HELIX   98   114  17      
STRAND   117   125  9      
HELIX   132   144  13      
TURN   145   147  3      
STRAND   149   155  7      
STRAND   160   163  4      
HELIX   166   175  10      
STRAND   179   183  5      
HELIX   185   187  3      
STRAND   188   190  3      
HELIX   191   208  18      
STRAND   211   216  6      
HELIX   226   237  12      
HELIX   240   242  3      
STRAND   243   247  5      
HELIX   249   253  5      
HELIX   256   269  14      
STRAND   272   275  4      
HELIX   277   283  7      
TURN   284   287  4      
HELIX   299   304  6      
STRAND   309   311  3      
HELIX   328   338  11      
HELIX   344   348  5      
HELIX   349   354  6      
HELIX   356   361  6      
STRAND   377   384  8      
HELIX   385   391  7      
STRAND   396   400  5      
STRAND   403   409  7      
STRAND   412   423  12      
Sequence information
Length: 427 AA [This is the length of the unprocessed precursor] Molecular weight: 47591 Da [This is the MW of the unprocessed precursor] CRC64: 9F91A2C46B3B1E42 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSNNALQTII NARLPGEEGL WQIHLQDGKI SAIDAQSGVM PITENSLDAE QGLVIPPFVE 

        70         80         90        100        110        120 
PHIHLDTTQT AGQPNWNQSG TLFEGIERWA ERKALLTHDD VKQRAWQTLK WQIANGIQHV 

       130        140        150        160        170        180 
RTHVDVSDAT LTALKAMLEV KQEVAPWIDL QIVAFPQEGI LSYPNGEALL EEALRLGADV 

       190        200        210        220        230        240 
VGAIPHFEFT REYGVESLHK TFALAQKYDR LIDVHCDEID DEQSRFVETV AALAHHEGMG 

       250        260        270        280        290        300 
ARVTASHTTA MHSYNGAYTS RLFRLLKMSG INFVANPLVN IHLQGRFDTY PKRRGITRVK 

       310        320        330        340        350        360 
EMLESGINVC FGHDDVFDPW YPLGTANMLQ VLHMGLHVCQ LMGYGQINDG LNLITHHSAR 

       370        380        390        400        410        420 
TLNLQDYGIA AGNSANLIIL PAENGFDALR RQVPVRYSVR GGKVIASTQP AQTTVYLEQP 


EAIDYKR 

P25524 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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