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UniProtKB/Swiss-Prot entry P25516


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ACON1_ECOLI
Primary accession number P25516
Secondary accession numbers P78060 P78148
Integrated into Swiss-Prot on May 1, 1992
Sequence was last modified on January 15, 2008 (Sequence version 3)
Annotations were last modified on    September 2, 2008 (Entry version 80)
Name and origin of the protein
Protein name Aconitate hydratase 1
Synonyms Aconitase 1
EC 4.2.1.3
Citrate hydro-lyase 1
Gene name
Name: acnA
Synonyms: acn
OrderedLocusNames: b1276, JW1268
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=1541275 [NCBI, ExPASy, EBI, Israel, Japan]
Prodromou C., Artymiuk P.J., Guest J.R.;
"The aconitase of Escherichia coli. Nucleotide sequence of the aconitase gene and amino acid sequence similarity with mitochondrial aconitases, the iron-responsive-element-binding protein and isopropylmalate isomerases.";
Eur. J. Biochem. 204:599-609(1992).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1093/dnares/3.6.363; PubMed=9097039 [NCBI, ExPASy, EBI, Israel, Japan]
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.;
"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.";
DNA Res. 3:363-377(1996).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[5]
PROTEIN SEQUENCE OF 2-19.
PubMed=1838390 [NCBI, ExPASy, EBI, Israel, Japan]
Prodromou C., Haynes M.J., Guest J.R.;
"The aconitase of Escherichia coli: purification of the enzyme and molecular cloning and map location of the gene (acn).";
J. Gen. Microbiol. 137:2505-2515(1991).
[6]
CHARACTERIZATION, MAGNETIC CIRCULAR DICHROISM, AND EPR SPECTROSCOPY.
DOI=10.1042/0264-6021:3440739; PubMed=10585860 [NCBI, ExPASy, EBI, Israel, Japan]
Jordan P.A., Tang Y., Bradbury A.J., Thomson A.J., Guest J.R.;
"Biochemical and spectroscopic characterization of Escherichia coli aconitases (AcnA and AcnB).";
Biochem. J. 344:739-746(1999).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X60293; CAA42834.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC74358.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAA14828.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR G64875; G64875.
RefSeq AP_001902.1; -.
NP_415792.1; -.
3D structure databases
ModBase P25516.
Protein-protein interaction databases
DIP DIP:9043N; -.
IntAct P25516; -.
Enzyme and pathway databases
BioCyc EcoCyc:ACONITASE-MON; -.
MetaCyc:ACONITASE-MON; -.
Organism-specific databases
EchoBASE EB1301; -.
EcoGene EG11325; acnA.
Ontologies
GO
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
IPR001030; Acoase/IPM_deHydtase_lsu_aba.
IPR015937; Aconitase-like_core.
IPR015928; Aconitase/3IPM_dehydase_swvl.
IPR006249; Aconitase/Fe_reg_prot_2.
IPR015934; Aconitase/Fe_reg_prot_2/AcnD.
IPR015932; Aconitase/IPMdHydase_lsu_aba_2.
IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
Graphical view of domain structure.
Gene3D G3DSA:3.30.499.10; Acnase/IPM_dHydase_lsu_aba_1/3; 2.
G3DSA:3.20.19.10; Aconitase/3IPM_dehydase_swvl; 1.
G3DSA:3.40.1060.10; Aconitase/IPMdHydase_lsu_aba_2; 1.
PANTHER PTHR11670; Aconitase-like_core; 1.
PTHR11670:SF1; Aconitase/Fe_reg_prot_2/AcnD; 1.
Pfam PF00330; Aconitase; 1.
PF00694; Aconitase_C; 1.
Pfam graphical view of domain structure.
PRINTS PR00415; ACONITASE.
ProDom PD000511; Aconitase_N; 1.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR01341; aconitase_1; 1.
PROSITE PS00450; ACONITASE_1; 1.
PS01244; ACONITASE_2; 1.
BLOCKS P25516.
Genome annotation databases
GeneID 946724; -.
GenomeReviews U00096_GR; b1276.
AP009048_GR; JW1268.
KEGG ecj:JW1268; -.
eco:b1276; -.
Phylogenomic databases
HOGENOM P25516; -.
Genome annotation databases
CMR P25516; b1276.
Other
ProtoNet P25516.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
4Fe-4S; Complete proteome; Direct protein sequencing; Iron; Iron-sulfur; Lyase; Metal-binding; Tricarboxylic acid cycle.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   891  890     Aconitate hydratase 1. PRO_0000076661
METAL   435   435        Iron-sulfur (4Fe-4S) (By similarity). 
METAL   501   501        Iron-sulfur (4Fe-4S) (By similarity). 
METAL   504   504        Iron-sulfur (4Fe-4S) (By similarity). 
CONFLICT   522   522        S -> G (in Ref. 1; CAA42834 and 4; BAA14828). 
Sequence information
Length: 891 AA [This is the length of the unprocessed precursor] Molecular weight: 97677 Da [This is the MW of the unprocessed precursor] CRC64: EB47E5B3C3F9C56C [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSSTLREASK DTLQAKDKTY HYYSLPLAAK SLGDITRLPK SLKVLLENLL RWQDGNSVTE 

        70         80         90        100        110        120 
EDIHALAGWL KNAHADREIA YRPARVLMQD FTGVPAVVDL AAMREAVKRL GGDTAKVNPL 

       130        140        150        160        170        180 
SPVDLVIDHS VTVDRFGDDE AFEENVRLEM ERNHERYVFL KWGKQAFSRF SVVPPGTGIC 

       190        200        210        220        230        240 
HQVNLEYLGK AVWSELQDGE WIAYPDTLVG TDSHTTMING LGVLGWGVGG IEAEAAMLGQ 

       250        260        270        280        290        300 
PVSMLIPDVV GFKLTGKLRE GITATDLVLT VTQMLRKHGV VGKFVEFYGD GLDSLPLADR 

       310        320        330        340        350        360 
ATIANMSPEY GATCGFFPID AVTLDYMRLS GRSEDQVELV EKYAKAQGMW RNPGDEPIFT 

       370        380        390        400        410        420 
STLELDMNDV EASLAGPKRP QDRVALPDVP KAFAASNELE VNATHKDRQP VDYVMNGHQY 

       430        440        450        460        470        480 
QLPDGAVVIA AITSCTNTSN PSVLMAAGLL AKKAVTLGLK RQPWVKASLA PGSKVVSDYL 

       490        500        510        520        530        540 
AKAKLTPYLD ELGFNLVGYG CTTCIGNSGP LPDPIETAIK KSDLTVGAVL SGNRNFEGRI 

       550        560        570        580        590        600 
HPLVKTNWLA SPPLVVAYAL AGNMNINLAS EPIGHDRKGD PVYLKDIWPS AQEIARAVEQ 

       610        620        630        640        650        660 
VSTEMFRKEY AEVFEGTAEW KGINVTRSDT YGWQEDSTYI RLSPFFDEMQ ATPAPVEDIH 

       670        680        690        700        710        720 
GARILAMLGD SVTTDHISPA GSIKPDSPAG RYLQGRGVER KDFNSYGSRR GNHEVMMRGT 

       730        740        750        760        770        780 
FANIRIRNEM VPGVEGGMTR HLPDSDVVSI YDAAMRYKQE QTPLAVIAGK EYGSGSSRDW 

       790        800        810        820        830        840 
AAKGPRLLGI RVVIAESFER IHRSNLIGMG ILPLEFPQGV TRKTLGLTGE EKIDIGDLQN 

       850        860        870        880        890 
LQPGATVPVT LTRADGSQEV VPCRCRIDTA TELTYYQNDG ILHYVIRNML K 

P25516 in FASTA format

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