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UniProtKB/Swiss-Prot entry P25464

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ACVS_CEPAC
Primary accession number P25464
Secondary accession numbers None
Integrated into Swiss-Prot on May 1, 1992
Sequence was last modified on May 1, 1992 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 59)
Name and origin of the protein
Protein name N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase
Synonyms EC 6.3.2.26
Delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase
ACV synthetase
ACVS
Gene name
Name: PCBAB
From
Cephalosporium acremonium (Acremonium chrysogenum) [TaxID: 5044] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Hypocreales; mitosporic Hypocreales; Acremonium.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE.
PubMed=1706706 [NCBI, ExPASy, EBI, Israel, Japan]
Gutierrez S., Diez B., Montenegro E., Martin J.F.;
"Characterization of the Cephalosporium acremonium pcbAB gene encoding alpha-aminoadipyl-cysteinyl-valine synthetase, a large multidomain peptide synthetase: linkage to the pcbC gene as a cluster of early cephalosporin biosynthetic genes and evidence of multiple functional domains.";
J. Bacteriol. 173:2354-2365(1991).
[2]
 PARTIAL NUCLEOTIDE SEQUENCE, AND PARTIAL PROTEIN SEQUENCE.
STRAIN=ATCC 11550 / CBS 779.69 / DSM 880 / IMI 49137;
DOI=10.1007/BF00327023; PubMed=2076552 [NCBI, ExPASy, EBI, Israel, Japan]
Hoskins J.A., O'Callaghan N., Queener S.W., Cantwell C.A., Wood J.S., Chen V.J., Skatrud P.L.;
"Gene disruption of the pcbAB gene encoding ACV synthetase in Cephalosporium acremonium.";
Curr. Genet. 18:523-530(1990).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
PIR A38531; YGCEVC.
3D structure databases
HSSP P14687; 1AMU. [HSSP ENTRY / PDB]
ModBase P25464.
Ontologies
GO
GO:0000036; Molecular function: acyl carrier activity (inferred from electronic annotation from InterPro).
GO:0048037; Molecular function: cofactor binding (inferred from electronic annotation from InterPro).
GO:0016788; Molecular function: hydrolase activity, acting on ester bonds (inferred from electronic annotation from InterPro).
GO:0050564; Molecular function: N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase activity (inferred from electronic annotation from EC).
GO:0031177; Molecular function: phosphopantetheine binding (inferred from electronic annotation from InterPro).
GO:0017000; Biological process: antibiotic biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR010071; AA_adenyl_dom.
IPR009081; ACP_like.
IPR000873; AMP-dep_Synth/Lig.
IPR001242; Condensatn.
IPR006163; Phsphopanteth_bd.
IPR006162; Ppantne_S.
IPR001031; Thioesterase.
Graphical view of domain structure.
Gene3D G3DSA:1.10.1200.10; ACP_like; 2.
Pfam PF00501; AMP-binding; 3.
PF00668; Condensation; 3.
PF00550; PP-binding; 3.
PF00975; Thioesterase; 1.
Pfam graphical view of domain structure.
PRINTS PR00154; AMPBINDING.
TIGRFAMs TIGR01733; AA-adenyl-dom; 3.
PROSITE PS50075; ACP_DOMAIN; 3.
PS00455; AMP_BINDING; 3.
PS00012; PHOSPHOPANTETHEINE; 2.
PROSITE graphical view of domain structure (profiles).
ProtoNet P25464.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Antibiotic biosynthesis; Direct protein sequencing; Ligase; Multifunctional enzyme; Phosphopantetheine; Repeat.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   3712  3712     N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase. PRO_0000193058
DOMAIN   795    864  70     Acyl carrier 1. 
DOMAIN   1880   1953  74     Acyl carrier 2. 
DOMAIN   2960   3027  68     Acyl carrier 3. 
REGION   234   1062  829     Domain 1 (adipate-activating). 
REGION   1335   2162  828     Domain 2 (cysteine-activating). 
REGION   2409   3387  979     Domain 3 (valine-activating). 
ACT_SITE   3568   3568        For thioesterase activity (By similarity). 
BINDING   827    827        Phosphopantetheine (covalent) (By similarity). 
BINDING   1916   1916        Phosphopantetheine (covalent) (By similarity). 
BINDING   2990   2990        Phosphopantetheine (covalent) (By similarity). 
Sequence information
Length: 3712 AA [This is the length of the unprocessed precursor] Molecular weight: 414775 Da [This is the MW of the unprocessed precursor] CRC64: 4EE3C1EB5EBEF9B7 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MALEQWKTTV QSVSERCDLS GLSQHPTDYQ LASTGVKGAG GSSIEERSAI VSDELFSSLR 

        70         80         90        100        110        120 
DVCSQRQLDP RSLMLFSVHQ MLKRFGNGSH TVVASLVTSS EGCPSTSAWR AIPSVIHHIE 

       130        140        150        160        170        180 
GGDNNNTVAS AVEQAANLLN SEGSGQDLLI PIGLTELVKS ELIDLLVIFD DETNNIRLPQ 

       190        200        210        220        230        240 
DFPLILRIHQ RQDHWQLSVR YPSPLFDTMV IDSFLSALHN LLSAVTKPSQ LVRDIELLPE 

       250        260        270        280        290        300 
YQVAQLEKWN NTDGDYPTEK RLHHLFEEAA VRRPQHVALI CGDKRITYEE LNAMANRLAH 

       310        320        330        340        350        360 
HLVSSGIQTE QLVGLFLDKT ELMIATILGI WKSGAAHVPI DPGYPDERVK FVLNDTKAQV 

       370        380        390        400        410        420 
VIASQRHVDR LRAEAVGGQH LRIIGLESLF DNLAQQTQHS PETSGNLTHL PLNSKQLAYV 

       430        440        450        460        470        480 
TYTSGTTGFP KGIYKEHTSV VNSITDLSAR YGVAGEDDEV ILVFSAYVFE PFVRQMLMAL 

       490        500        510        520        530        540 
TTGNSLAIIS DEDKFDPDTL IPFIQKHKVT YIHATSSVLQ EYDFGSCPSL KRMILVGENL 

       550        560        570        580        590        600 
TEPRYEALRQ RFKSRILNEY GFTESAFVTA LNIFEPTSQR KDMSLGRPVR NVKCYILDAN 

       610        620        630        640        650        660 
LKRVPIGVTG ELHIGGLGIS RGYMNREELT RQKFLPNPYQ TDKERQRGVN STMYKTGDLA 

       670        680        690        700        710        720 
RWLPSGEVEY LGRADFQIKL RGIRIEPGEI ESTLAMYPGI RASIVVSKKL LSQGQETIQD 

       730        740        750        760        770        780 
HLVGYYVCDE GHIPEGDLLS FLEKKLPRYM VPTRLVQLAQ IPTNINGKAD LRALPAVEVA 

       790        800        810        820        830        840 
VAPTHKQDGE RGNQLESDLA AIWGNILSVP AQDIGSESNF FRLGGHSIAC IQLIARVRQQ 

       850        860        870        880        890        900 
LGQGITLEEV FQTKTLRAMA ALLSEKYTKA SNGTNGVTNG TAHVNGHAAN GHVSDSYVAS 

       910        920        930        940        950        960 
SLQQGFVYHS LKNELSEAYT MQSMIHYGVP LKRDIYQAAW QRVQGEHPAL RLRFTWEAEV 

       970        980        990       1000       1010       1020 
MQIVDPKSEL DWRVVDWTDV SSREKQLVAL EQLQTEDLAK VYHLDKGPLM RLYLILLPDS 

      1030       1040       1050       1060       1070       1080 
KYSCLFSCHH AILDGWSLPL LFNNVHQAYL DLVEGTASPV EQDATYLLGQ QYLQSHRDDH 

      1090       1100       1110       1120       1130       1140 
LDFWAEQIGR IEERCDMNAL LNEASRYKVP LADYDQVREQ RQQTISLPWN NSMDAGVREE 

      1150       1160       1170       1180       1190       1200 
LSSRGITLHS ILQTVWHLVL HSYGGGTHTI TGTTISGRHL PVPGIERSVG LFINTLPMIF 

      1210       1220       1230       1240       1250       1260 
DHTVCQDMTA LEAIEHVQGQ VNAMNSRGNV ELGRMSKNDL KHGLFDTLFV LENYPNLDTE 

      1270       1280       1290       1300       1310       1320 
QREKHEEKLK FTIKGGTEKL SYPLAVIAQE DGDSGCSFTL CYAGELFTDE SIQALLDTVR 

      1330       1340       1350       1360       1370       1380 
DTLSDILGNI HAPIRNMEYL SSNQTAQLDK WNATAFEYPN TTLHAMFESE AQQKPDKVAV 

      1390       1400       1410       1420       1430       1440 
VYEDIRLTYR ELNSRANALA FYLLSQAAIQ PNKLVGLIMD KSEHMITSIL AVWKTGGAYV 

      1450       1460       1470       1480       1490       1500 
PIDPRYPDQR IQYILEDTAA LAVITDSPHI DRLRSITNNR LPVIQSDFAL QLPPSPVHPV 

      1510       1520       1530       1540       1550       1560 
SNCKPSDLAY IMYTSGTTGN PKGVMVEHHG VVNLCVSLCR LFGLRNTDDE VILSFSNYVF 

      1570       1580       1590       1600       1610       1620 
DHFVEQMTDA LLNGQTLVVL NDEMRGDKER LYRYIETNRV TYLSGTPSVI SMYEFDRFRD 

      1630       1640       1650       1660       1670       1680 
HLRRVDCVGE AFSEPVFDKI RETFPGLIIN GYGPTEVSIT THKRPYPFPE RRTDKSIGCQ 

      1690       1700       1710       1720       1730       1740 
LDNSTSYVLN DDMKRVPIGA VGELYLGGDG VARGYHNRPD LTADRFPANP FQTEQERLEG 

      1750       1760       1770       1780       1790       1800 
RNARLYKTGD LVRWIHNANG DGEIEYLGRN DFQVKIRGQR IELGEIEAVL SSYPGIKQSV 

      1810       1820       1830       1840       1850       1860 
VLAKDRKNDG QKYLVGYFVS SAGSLSAQAI RRFMLTSLPD YMVPAQLVPI AKFPVTVSGK 

      1870       1880       1890       1900       1910       1920 
LDAKALPVPD DTVEDDIVPP RTEVERILAG IWSELLEIPV DRISIYSDFF SLGGDSLKST 

      1930       1940       1950       1960       1970       1980 
KLSFAATRAL GVAVSVRNLF SHPTIEALSQ WIIRGSNEVK DVAVVKGGAS LDIPLSPAQE 

      1990       2000       2010       2020       2030       2040 
RLMFIHEFGH SGEDTGAYNV PLQLQLHHDV CLESLEKALR DVVSRHEALR TLITRTQKSS 

      2050       2060       2070       2080       2090       2100 
VHCQKILDAE EAQKLFSVDV LRLTSETEMQ GRMAESTAHA FKLDEELPIH VRLYQVVRDG 

      2110       2120       2130       2140       2150       2160 
RTLSFASIVC HHLAFDAWSW DVFQRDLDAF YAVHTKHKAA ANLPTLRVQY KEYAIEHRRA 

      2170       2180       2190       2200       2210       2220 
LRAEQHRVLA DYWLRKLSDM EASYLVPDRP RPAQFDYTGN DLQFSTTPET TAQLKELAKR 

      2230       2240       2250       2260       2270       2280 
EGSSLYTVVA AAYFLLLYVY TNQRDITIGI PVAHRNHPDF ESVVGFFVNL LPLRVNVSQS 

      2290       2300       2310       2320       2330       2340 
DIHGLIQAVQ KELVDAQIHQ DLPFQEITKL LHVQHDPSRH PLLQAVFNWE NVPANVHEEQ 

      2350       2360       2370       2380       2390       2400 
LLQEYKPPSP LPSAAKFDLN VTVKESVNSL NVNFNYPTSL FEEETVQGFM ETFHLLLRQL 

      2410       2420       2430       2440       2450       2460 
AHNKASTSLS KLSVEDGVLN PEPTNLQPSS RDSGNSLHGL FEDIVASTPD RIAIADGTRS 

      2470       2480       2490       2500       2510       2520 
LSYSELNERA NQLVHLIISS ASIVADDRIA LLLDKSIDMV IALLAVWKAG AAYVPLDPTY 

      2530       2540       2550       2560       2570       2580 
PSQRTELILE ESSARTLITT RKHTPRGGTV ANVPSVVLDS PETLACLNQQ SKENPTTSTQ 

      2590       2600       2610       2620       2630       2640 
KPSDLAYVIF TSGTTGKPKG VLVEHQSVVQ LRNSLIERYF GETNGSHAVL FLSNYVFDFS 

      2650       2660       2670       2680       2690       2700 
LEQLCLSVLG GNKLIIPPEE GLTHEAFYDI GRREKLSYLS GTPSVLQQIE LSRLPHLHMV 

      2710       2720       2730       2740       2750       2760 
TAAGEEFHAS QFEKMRSQFA GQINNAYGIT ETTVYNIITT FKGDAPFTKA LCHGIPGSHV 

      2770       2780       2790       2800       2810       2820 
YVLNDRLQRV PFNAVGELYL GGDCLARGYL NQDALTNERF IPNPFYEPKQ ASDSRPQRLY 

      2830       2840       2850       2860       2870       2880 
KTGDLVRFRG PHHLEYLGRK DQQVKLRGFR IELSEVRDAV LAISAVKEAA VIPKYDEDGS 

      2890       2900       2910       2920       2930       2940 
DSRRVSAIVC YYTLNAGTVC EASSIRDHLH ANLPPYMVPS QIHQLEGSLP VTVNGKLDLN 

      2950       2960       2970       2980       2990       3000 
RLSTTQVSQP ELYTAPRNST EETLCQLWAS LLGVDHCGID DDLFARGGDS ISSLRLVGDI 

      3010       3020       3030       3040       3050       3060 
YRALGRKVTV KDIYLHRSVR ALSENVLTDQ KDKGTLPASP PLQRAEQGQV EGDAPLLPIQ 

      3070       3080       3090       3100       3110       3120 
DWFLSKPLDN PAYWNHCFTI RTGALSVEGL RGALKLLQER HDVLRLRLQR RDEGRHVQTF 

      3130       3140       3150       3160       3170       3180 
ARDCAQPRLT VLDRRSFEDA EDVQEALCEI QSHFDLENGP LYTVAYIHGY EDGSARVWFA 

      3190       3200       3210       3220       3230       3240 
CHHVMVDTVS WNIILQDLQA LYHGDSLGPK SSSVQQWSLA VSDYKMPLSE RAHWNVLRKT 

      3250       3260       3270       3280       3290       3300 
VAQSFETLPI CMGGVLQCQE KFSRETTTAL LSKACPALDS GMHEILLMAV GSALQKAAGD 

      3310       3320       3330       3340       3350       3360 
VPQVVTIEGH GREDTIDATL DVSRTVGWFT SMYPFEIPKV TDPAQGVVDV KEAMRRVPNR 

      3370       3380       3390       3400       3410       3420 
GVGYGPAYGY GGSCLPAVSF NYLGRLDQAS SGAQRDWTLV MDEDEYPVGL CTSAEDSGRS 

      3430       3440       3450       3460       3470       3480 
SSMVDFTFSI SGGQLVMDMS SSWGHGARNE FVRTVRNTLD DLIKTTSSRD FSAPLPPSDQ 

      3490       3500       3510       3520       3530       3540 
ESSFTPYFVF EEGERHGAPL FLLPPGEGGA ESYFHNIVKG LPNRNLVVFN NHYREEKTLR 

      3550       3560       3570       3580       3590       3600 
TIEALAEYYL SHIRSIQPEG PYHILGWSFG GILGLEAAKR LTGEGHKIAT LALIDPYFDI 

      3610       3620       3630       3640       3650       3660 
PSASKAIGQP DDACVLDPIY HVYHPSPESF RTVSSLTNHI ALFKATETND QHGNATQQAL 

      3670       3680       3690       3700       3710 
YEWFATCPLN NLDKFLAADT IKVVPLEGTH FTWVHHPEQV RSMCTMLDEW LG
P25464 in FASTA format

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