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UniProtKB/Swiss-Prot entry P25375


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PRTD_YEAST
Primary accession number P25375
Secondary accession numbers None
Integrated into Swiss-Prot on May 1, 1992
Sequence was last modified on May 1, 1992 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 77)
Name and origin of the protein
Protein name Saccharolysin
Synonyms EC 3.4.24.37
Protease D
Proteinase yscD
Oligopeptidase YSCD
Gene name
Name: PRD1
OrderedLocusNames: YCL057W
ORFNames: YCL57W
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=8307027 [NCBI, ExPASy, EBI, Israel, Japan]
Buechler M., Tisljar U., Wolf D.H.;
"Proteinase yscD (oligopeptidase yscD). Structure, function and relationship of the yeast enzyme with mammalian thimet oligopeptidase (metalloendopeptidase, EP 24.15).";
Eur. J. Biochem. 219:627-639(1994).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
DOI=10.1038/357038a0; PubMed=1574125 [NCBI, ExPASy, EBI, Israel, Japan]
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.;
"The complete DNA sequence of yeast chromosome III.";
Nature 357:38-46(1992).
[3]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[4]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73, AND MASS SPECTROMETRY.
DOI=10.1021/pr060559j; PubMed=17330950 [NCBI, ExPASy, EBI, Israel, Japan]
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.;
"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.";
J. Proteome Res. 6:1190-1197(2007).
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73 AND SER-75, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X76504; CAA54039.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X59720; CAA42388.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S19387; S19387.
RefSeq NP_009874.1; -.
3D structure databases
HSSP P42676; 1I1I. [HSSP ENTRY / PDB]
ModBase P25375.
Protein-protein interaction databases
DIP DIP:4938N; -.
IntAct P25375; -.
Protein family/group databases
MEROPS M03.003; -.
Organism-specific databases
CYGD YCL057w; -.
SGD S000000562; PRD1.
Yeast-GFP YCL057W.
Gene expression databases
ArrayExpress P25375; -.
GermOnline YCL057W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005758; Cellular component: mitochondrial intermembrane space (inferred from direct assay from SGD).
GO:0004222; Molecular function: metalloendopeptidase activity (inferred from electronic annotation from InterPro).
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from InterPro).
GO:0006508; Biological process: proteolysis (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR001567; Pept_M3A_M3B.
IPR006025; Pept_M_Zn_BS.
Graphical view of domain structure.
Pfam PF01432; Peptidase_M3; 1.
Pfam graphical view of domain structure.
PROSITE PS00142; ZINC_PROTEASE; 1.
ProtoNet P25375.
Proteomic databases
PeptideAtlas P25375; -.
Genome annotation databases
Ensembl YCL057W; Saccharomyces cerevisiae. [Contig view]
GeneID 850301; -.
GenomeReviews X59720_GR; YCL057W.
KEGG sce:YCL057W; -.
NMPDR fig|4932.3.peg.594; -.
Phylogenomic databases
HOGENOM P25375; -.
Other
LinkHub P25375; -.
NextBio 965679; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytoplasm; Hydrolase; Metal-binding; Metalloprotease; Phosphoprotein; Protease; Zinc.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   712  712     Saccharolysin. PRO_0000078156
ACT_SITE   502   502        By similarity. 
METAL   501   501        Zinc; catalytic (By similarity). 
METAL   505   505        Zinc; catalytic (By similarity). 
METAL   508   508        Zinc; catalytic (By similarity). 
MOD_RES   73    73        Phosphoserine. 
MOD_RES   75    75        Phosphoserine. 
Sequence information
Length: 712 AA [This is the length of the unprocessed precursor] Molecular weight: 81934 Da [This is the MW of the unprocessed precursor] CRC64: 340910B7FDAFBE37 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MRLLLCKNWF ASPVISPLLY TRSLYSMANT TSFPIAPQAP PNWSFTPSDI SGKTNEIINN 

        70         80         90        100        110        120 
SNNFYDSMSK VESPSVSNFV EPFMKFENEL GPIINQLTFL QHVSSDKEIR DASVNSSMKL 

       130        140        150        160        170        180 
DELNIDLSLR HDIFLQFARV WQDVQSKADS VERETFKYVE KSYKDYIHSG LELDEGNRLK 

       190        200        210        220        230        240 
IKEIKKKISV NSINFSKNLG EQKEYITFTK EQLEGVPDSI LTQFETIKSD KDSNETLYKV 

       250        260        270        280        290        300 
TFKYPDIFPV MKLASSAQTR KQAFLADQNK VPENEAILLD TLKLRDELAS LLGYDTYANY 

       310        320        330        340        350        360 
NLYDKMAEDS TTVMNFLNDL KDKLIPLGRK ELQVLQDMKA EDVKKLNQGA DPNYYIWDHR 

       370        380        390        400        410        420 
YYDNKYLLEN FNVDLEKISE YFPLEATITG MLEIYETLFN LKFIETKDSQ NKSVWHDDVK 

       430        440        450        460        470        480 
QIAVWNMDDP KSPNFVGWIY FDLHPRDGKY GHAANFGLSS SFMIDDTTRS YPVTALVCNF 

       490        500        510        520        530        540 
SKSTKDKPSL LKHNEIVTFF HELGHGIHDL VGQNKESRFN GPGSVPWDFV EAPSQMLEFW 

       550        560        570        580        590        600 
TWNKNELINL SSHYKTGEKI PESLINSLIK TKHVNGALFT LRQLHFGLFD MKVHTCKDLQ 

       610        620        630        640        650        660 
NLSICDTWNQ LRQDISLISN GGTLSKGYDS FGHIMSDSYS AGYYGYLWAE VFATDMYHTK 

       670        680        690        700        710 
FAKDPLNAKN GIQYRDIVLA RGGLYDINDN LKEFLGREPS KDAFLKELGL QN 

P25375 in FASTA format

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