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UniProtKB/Swiss-Prot entry P25282

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MTG1_HAEGA
Primary accession number P25282
Secondary accession numbers None
Integrated into Swiss-Prot on May 1, 1992
Sequence was last modified on February 1, 1994 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 53)
Name and origin of the protein
Protein name Modification methylase HgaIA
Synonyms M.HgaIA
EC 2.1.1.37
Cytosine-specific methyltransferase HgaIA
M.HgaI-1
Gene name
Name: hgaIAM
From
Haemophilus gallinarum [TaxID: 728] 
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Avibacterium.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=NCTC 3438;
PubMed=1856224 [NCBI, ExPASy, EBI, Israel, Japan]
Sugisaki H., Yamamoto K., Takanami M.;
"The HgaI restriction-modification system contains two cytosine methylase genes responsible for modification of different DNA strands.";
J. Biol. Chem. 266:13952-13957(1991).
[2]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=NCTC 3438;
Sugisaki H.;
"Nucleotide sequence of the gene of HgaI restriction endonuclease.";
Bull. Inst. Chem. Res., Kyoto Univ. 71:338-342(1993).
Comments
  • FUNCTION: This methylase recognizes the strands carrying the 5'-GCGTC-3' sequence, causes specific methylation on C-2, and protects the DNA from cleavage by the HgaI endonuclease.
  • CATALYTIC ACTIVITY: S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
  • MISCELLANEOUS: The HgaI modification system consists of two cytosine methylase genes responsible for modification of different strands in the target DNA.
  • SIMILARITY: Belongs to the C5-methyltransferase family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
D90363; BAA14377.1; ALT_INIT; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
D17388; BAA04206.1; ALT_INIT; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B39467; B39467.
3D structure databases
ModBase P25282.
Protein family/group databases
REBASE 3650; M1.HgaI.
Ontologies
GO
GO:0003886; Molecular function: DNA (cytosine-5-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0003677; Molecular function: DNA binding (inferred from electronic annotation from InterPro).
GO:0006306; Biological process: DNA methylation (inferred from electronic annotation from InterPro).
GO:0009307; Biological process: DNA restriction-modification system (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001525; C5_DNA_meth.
Graphical view of domain structure.
PANTHER PTHR10629; C5_DNA_meth; 1.
Pfam PF00145; DNA_methylase; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00675; dcm; 1.
PROSITE PS00094; C5_MTASE_1; 1.
PS00095; C5_MTASE_2; FALSE_NEG.
BLOCKS P25282.
ProtoNet P25282.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Methyltransferase; Restriction system; S-adenosyl-L-methionine; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom  To Length Description FTId
CHAIN   1   357  357     Modification methylase HgaIA. PRO_0000087879
ACT_SITE   83    83        By similarity. 
Sequence information
Length: 357 AA [This is the length of the unprocessed precursor] Molecular weight: 40863 Da [This is the MW of the unprocessed precursor] CRC64: 9F3E10D620AF3154 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MKKNIMGLSL FSSAGIGEYF LSRVGIDIIV ANELIKKRAD LYQKIYPNHK MVIGDIRDQR 

        70         80         90        100        110        120 
IFNKVLNIAL TNQVDFLIAS PPCQGMSVAG KNRDVSNMAN DNRNYLIMYV IAMIKKLKPA 

       130        140        150        160        170        180 
YILIENVPFL LKLELYIDNK LTPIKNILED EFGSEYHIHF DILDAADYGT PQRRKRAIIR 

       190        200        210        220        230        240 
LNKKGTIWNL PLKQNIVSVE QAIGNLPSIE SGKHSGLKWH YGRGHTEQQI EWMKHTPTGK 

       250        260        270        280        290        300 
SAFENLVHYP RKANGEKVKG YHSSYRRIRW DEPAPTITIR NDAISSQRNV HPGRPLLDGT 

       310        320        330        340        350 
YSDARVLSVL ELMRLTGLPD NWEIPDDTPE ILIRQIIGEC IPPLLIENIT REIFNEN
P25282 in FASTA format

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