[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=HPA2; DOI=10.1016/0378-1119(91)90569-W; PubMed=1937045 [NCBI, ExPASy, EBI, Israel, Japan] Duesterhoeft A., Kroeger M.; "Cloning, sequence and characterization of m5C-methyltransferase-encoding gene, hgiDIIM (GTCGAC), from Herpetosiphon giganteus strain Hpa2."; Gene 106:87-92(1991).
[2]	DISCUSSION OF SEQUENCE. DOI=10.1016/0378-1119(94)00779-R; PubMed=7607523 [NCBI, ExPASy, EBI, Israel, Japan] Kroeger M., Blum E., Deppe E., Duesterhoeft A., Erdmann D., Kilz S., Meyer-Rogge S., Moestl D.; "Organization and gene expression within restriction-modification systems of Herpetosiphon giganteus."; Gene 157:43-47(1995).

Comments

FUNCTION: This methylase recognizes the double-stranded sequence GTCGAC, causes specific methylation on C-? on both strands and protects the DNA from cleavage by the HgiDII endonuclease.
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
SIMILARITY: Belongs to the C5-methyltransferase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X55141; CAA38941.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S21949; JT0594.

3D structure databases

ModBase

P25265.

Protein family/group databases

REBASE

3418; M.HgiDII.

Ontologies

GO:0003886;	Molecular function: DNA (cytosine-5-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0003677;	Molecular function: DNA binding (inferred from electronic annotation from InterPro).
GO:0006306;	Biological process: DNA methylation (inferred from electronic annotation from InterPro).
GO:0009307;	Biological process: DNA restriction-modification system (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001525; C5_DNA_meth.
Graphical view of domain structure.

PANTHER

PTHR10629; C5_DNA_meth; 1.

Pfam

PF00145; DNA_methylase; 1.
Pfam graphical view of domain structure.

PRINTS

PR00105; C5METTRFRASE.

TIGRFAMs

TIGR00675; dcm; 1.

PROSITE

PS00094; C5_MTASE_1; 1.
PS00095; C5_MTASE_2; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Methyltransferase; Restriction system; S-adenosyl-L-methionine; Transferase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 354`	`354`	`Modification methylase HgiDII.`	`PRO_0000087889`
`ACT_SITE`	`79 79`		`By similarity.`

Sequence information

Length: 354 AA [This is the length of the unprocessed precursor]

Molecular weight: 39853 Da [This is the MW of the unprocessed precursor]

CRC64: BDA017785D5ADC75 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVGAVIDLFC GVGGLTHGLI LEGFGVLAGI DNDPSCKYAY EQNNRTRFIE KSISEVDGRE 

        70         80         90        100        110        120 
LNALYPNNQH KILVGCAPCQ DFSQYTKKSR TGTKWQLLTE FSRLIREIEP DIISMENVPE 

       130        140        150        160        170        180 
VRTFNRGEVF NNFIQSLEQL GYHVSHSVVH CPDYGIPQQR DRLVLFAAKQ GVIKIIPPTH 

       190        200        210        220        230        240 
TPENYRTVRD VIGSLATNYS GGHWEGDSMH AASRLEDINL RRIQHSVPGG TWADWPEELI 

       250        260        270        280        290        300 
AECHKKESGE SYGSVYGRME WDKVAPTITT QCNGYGNGRF GHPEQDRAIS LREAALLQTF 

       310        320        330        340        350 
PRSYQFAPEG QLKFKTVSRQ IGNAVPVALG RVIAKSIKRF LEGLHERQRV RIII

P25265 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools