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UniProtKB/Swiss-Prot entry P25202

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name RPC1_GIALA
Primary accession number P25202
Secondary accession numbers None
Integrated into Swiss-Prot on May 1, 1992
Sequence was last modified on May 1, 1992 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 52)
Name and origin of the protein
Protein name DNA-directed RNA polymerase III subunit RPC1
Synonyms RNA polymerase III subunit C1
EC 2.7.7.6
DNA-directed RNA polymerase III largest subunit
C160
Gene name
Name: RPOA3
From
Giardia lamblia (Giardia intestinalis) [TaxID: 5741] 
Taxonomy Eukaryota; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=SSP. P1;
DOI=10.1093/nar/20.5.1145; PubMed=1549483 [NCBI, ExPASy, EBI, Israel, Japan]
Lanzendoerfer M., Palm P., Grampp B., Peattie D.A., Zillig W.;
"Nucleotide sequence of the gene encoding the largest subunit of the DNA-dependent RNA polymerase III of Giardia lamblia.";
Nucleic Acids Res. 20:1145-1145(1992).
Comments
  • FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).
  • CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
  • SUBUNIT: Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity).
  • SUBCELLULAR LOCATION: Nucleus (By similarity).
  • SIMILARITY: Belongs to the RNA polymerase beta' chain family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X60325; CAA42895.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S22812; S22812.
3D structure databases
HSSP P04050; 1I6H. [HSSP ENTRY / PDB]
ModBase P25202.
Ontologies
GO
GO:0005634; Cellular component: nucleus (inferred from electronic annotation from UniProtKB-KW).
GO:0003677; Molecular function: DNA binding (inferred from electronic annotation from InterPro).
GO:0003899; Molecular function: DNA-directed RNA polymerase activity (inferred from electronic annotation from InterPro).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008270; Molecular function: zinc ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006350; Biological process: transcription (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR015700; RNA_pol3.
IPR000722; RNA_pol_A.
IPR006592; RNA_pol_N.
IPR007080; RNA_pol_Rpb1_1.
IPR007066; RNA_pol_Rpb1_3.
IPR007083; RNA_pol_Rpb1_4.
IPR007081; RNA_pol_Rpb1_5.
Graphical view of domain structure.
Gene3D G3DSA:2.40.40.30; RNA_pol_A; 1.
G3DSA:3.90.1120.10; RNA_pol_Rpb1_1; 1.
PANTHER PTHR19376:SF15; RNA_pol3; 1.
Pfam PF04997; RNA_pol_Rpb1_1; 1.
PF00623; RNA_pol_Rpb1_2; 1.
PF04983; RNA_pol_Rpb1_3; 1.
PF05000; RNA_pol_Rpb1_4; 1.
PF04998; RNA_pol_Rpb1_5; 1.
Pfam graphical view of domain structure.
SMART SM00663; RPOLA_N; 1.
SMART graphical view of domain structure.
BLOCKS P25202.
ProtoNet P25202.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
DNA-directed RNA polymerase; Magnesium; Metal-binding; Nucleotidyltransferase; Nucleus; Transcription; Transferase; Zinc; Zinc-finger.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   1741  1741     DNA-directed RNA polymerase III subunit RPC1. PRO_0000073948
ZN_FING   79     92  14     C3H1-type (Potential). 
REGION   1099   1111  13     Bridging helix (By similarity). 
METAL   79     79        Zinc 1 (By similarity). 
METAL   82     82        Zinc 1 (By similarity). 
METAL   89     89        Zinc 1 (By similarity). 
METAL   92     92        Zinc 1 (By similarity). 
METAL   119    119        Zinc 2 (By similarity). 
METAL   122    122        Zinc 2 (By similarity). 
METAL   160    160        Zinc 2 (By similarity). 
METAL   722    722        Magnesium; catalytic (By similarity). 
METAL   724    724        Magnesium; catalytic (By similarity). 
METAL   726    726        Magnesium; catalytic (By similarity). 
Sequence information
Length: 1741 AA [This is the length of the unprocessed precursor] Molecular weight: 193929 Da [This is the MW of the unprocessed precursor] CRC64: DE2EF212CBCF6CD8 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MEPNRRLRYF RSEIPQPVTE HRFPKRVEKL VFSVLSSNAL MKLSEVQITT PILYEHYPSP 

        70         80         90        100        110        120 
ATNGVLDKRL GLSDKVGKCA TCGKDTKMCI GHFGTISLAL PVFHPGLIND ARTMLSIVCP 

       130        140        150        160        170        180 
FCSRVKISEA DRLKYKVLNH KFSLAIVKEA QEQAKKQVNC PFCHAPSTIC KKSQGFRVLR 

       190        200        210        220        230        240 
EMHYEERATK ILADVIKHQK DIEKIQELDQ RLRNHVVDPI QALHILQKVP ECDYPYLGIP 

       250        260        270        280        290        300 
WQSRNVYASQ QTPDSIASND ASQLFSRGSS KLLARTASRF IAQHQGISDR ATRLTSHAFL 

       310        320        330        340        350        360 
TRPQDVILTH IPVPPSCLRP SVQSGSGAGT TEDNLTSHLV RILTINDKLK EAMVSGAVAT 

       370        380        390        400        410        420 
SKVYAKWDQL NMDVSLLYIG KIPGGVQPVK NKTDSGTKEG TGIIDRLKGK AGRFRSNLSG 

       430        440        450        460        470        480 
KRVNFSGRTV ISPNPYLSMQ QVAIPRLIAQ NLTFPEIVTS RNIRFLRELI LNGKTYPGAN 

       490        500        510        520        530        540 
EVLLLNDTIR YNPALKRLGI DVKQSRDMEA KAMELVSRCV DTFNLNSLCA DLPELQAAIQ 

       550        560        570        580        590        600 
HLVSESSNAI NSELKAEDLP QNQPLFAKEV YDPAQKGESN NIDNNVCEID ELKLEDTADY 

       610        620        630        640        650        660 
QVGESAGQAS TVAVEQPPES RTVLKLNSIA SLQLTNASSQ HLSTFKLNTV SLLQKDRRGR 

       670        680        690        700        710        720 
QAIANSLRPN DIVYRHLLPN DTLLFNRQPS LHRMSIMQFN AVIHENRTFS FNPVVCSPFN 

       730        740        750        760        770        780 
ADFDGDEMNI FYMQGQEARA EAGILMGSHE NIISPRHGEC MIGLTQDFLT GIYLLSGKGI 

       790        800        810        820        830        840 
FMTRQEYCQH VSYGCDGFGD ATYGVSLYNY GREFIKSIHD RRGTEEDTVG FVKVPCLSQP 

       850        860        870        880        890        900 
CIVYPRQLYS GKQVLSVLMK GNDFDSVNIN LEHGDKTYKK DSDRRALSVN DDYIIIQNSE 

       910        920        930        940        950        960 
HLVGRLTKTF LASSKNCIFY FLVQNYGPVS AARIMLRFAK VAARFLMNYG FTIGIDDVMP 

       970        980        990       1000       1010       1020 
SQRVLGKKEV IVQEGYEKAQ EKIRDYESGK LEAIPGSTVQ ETLEATLNQI LSNVRESCAQ 

      1030       1040       1050       1060       1070       1080 
IALKELHFTN KPLIMSLCGS KGSPINIAQM IIILGQQSFG GSRAPDDFYT RSTPYFYHYS 

      1090       1100       1110       1120       1130       1140 
KEPNAKGFIL NSFYTGLLPF EFLAHARAGR DGVIDSACKT ADTGYLQRRL VKCLEDLSVS 

      1150       1160       1170       1180       1190       1200 
YDFTVRNSKK SVVQFRFGHD GFDPIKLEVG TGQCFDLDTL LRHIILLNRS QDLAEGAFPV 

      1210       1220       1230       1240       1250       1260 
LLDRYKAEAE LDVLLNKDFY GQEACLIERS NVTSYFQQSI LPKLTTNTHI ATTVAPALAA 

      1270       1280       1290       1300       1310       1320 
SFLQTTLTKK DIDDFLRQVR RKYIRLNLEP GSPCGAVAAQ SVGEPSTQMT LKSFHHAGLA 

      1330       1340       1350       1360       1370       1380 
SMNITQGVPR LKEIVDGVVK ISTPITTVEL HVDVDKEELA ATVTEKYLEK ARMMKNIIEC 

      1390       1400       1410       1420       1430       1440 
TYLGQISASI IECYSQSVCH IEVNLDMKII ADMGLAGIIT VETVVASILH NDKAKKLVGQ 

      1450       1460       1470       1480       1490       1500 
DQSSGSVSIH SATRFSVRPL TQSRDDLLFD IQSLKLLLPM IPVSGISTCS RAVINEYKEF 

      1510       1520       1530       1540       1550       1560 
CQGATSSAPL TKYNLLVEGI GLQNILNVSG IDFTRTLSNN IVEVANTLGI EAAVATISNE 

      1570       1580       1590       1600       1610       1620 
IKACMDSHGM AVDMRHIRLL ADIMCFRGRV LGFTRFGLTK MKADSVIMLA SFEKTGEHLF 

      1630       1640       1650       1660       1670       1680 
NAALGNKVDE ANGVTESIIL GKPMSMGTGS FSLLQAPYFD EKTGKTIEYQ PKQTTRFLGE 

      1690       1700       1710       1720       1730       1740 
VLNKQYDEEV DAIVNAFWYD EKLYLTGAEA KAKLLRGRRH ANKRSWSRGK ERHASLKPKN 


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P25202 in FASTA format

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