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UniProtKB/Swiss-Prot entry P25171


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name RCC1_DROME
Primary accession number P25171
Secondary accession numbers A4V1J1 Q9VRR5
Integrated into Swiss-Prot on May 1, 1992
Sequence was last modified on June 1, 2001 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 78)
Name and origin of the protein
Protein name Regulator of chromosome condensation
Synonym Chromatin-binding protein Bj1
Gene name
Name: Bj1
ORFNames: CG10480
From
Drosophila melanogaster (Fruit fly) [TaxID: 7227] 
Taxonomy Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=2022188 [NCBI, ExPASy, EBI, Israel, Japan]
Frasch M.;
"The maternally expressed Drosophila gene encoding the chromatin-binding protein BJ1 is a homolog of the vertebrate gene Regulator of Chromatin Condensation, RCC1.";
EMBO J. 10:1225-1236(1991).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Berkeley;
DOI=10.1126/science.287.5461.2185; PubMed=10731132 [NCBI, ExPASy, EBI, Israel, Japan]
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
"The genome sequence of Drosophila melanogaster.";
Science 287:2185-2195(2000).
[3]
GENOME REANNOTATION.
PubMed=12537572 [NCBI, ExPASy, EBI, Israel, Japan]
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.";
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=Berkeley;
TISSUE=Embryo;
PubMed=12537569 [NCBI, ExPASy, EBI, Israel, Japan]
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.;
"A Drosophila full-length cDNA resource.";
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
[5]
CHARACTERIZATION, AND SUBCELLULAR LOCATION.
PubMed=2022190 [NCBI, ExPASy, EBI, Israel, Japan]
Ohtsubo M., Yoshida T., Seino H., Nishitani H., Clark K.L., Sprague G.F. Jr., Frasch M., Nishimoto T.;
"Mutation of the hamster cell cycle gene RCC1 is complemented by the homologous genes of Drosophila and S.cerevisiae.";
EMBO J. 10:1265-1273(1991).
[6]
SUBCELLULAR LOCATION.
DOI=10.1016/S0960-9822(02)00934-X; PubMed=12121620 [NCBI, ExPASy, EBI, Israel, Japan]
Trieselmann N., Wilde A.;
"Ran localizes around the microtubule spindle in vivo during mitosis in Drosophila embryos.";
Curr. Biol. 12:1124-1129(2002).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-286; THR-512; SER-526 AND THR-531, AND MASS SPECTROMETRY.
TISSUE=Embryo;
DOI=10.1021/pr700696a; PubMed=18327897 [NCBI, ExPASy, EBI, Israel, Japan]
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
"Phosphoproteome analysis of Drosophila melanogaster embryos.";
J. Proteome Res. 7:1675-1682(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X58530; CAA41417.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE014296; AAF50726.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE014296; AAF50727.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE014296; AAN12235.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY070540; AAL48011.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S15028; S15028.
RefSeq NP_523943.1; -.
NP_729118.1; -.
NP_729119.1; -.
UniGene Dm.8023
3D structure databases
HSSP P18754; 1I2M. [HSSP ENTRY / PDB]
ModBase P25171.
Protein-protein interaction databases
DIP DIP:17766N; -.
IntAct P25171; -.
Enzyme and pathway databases
BioCyc DMEL-XXX-02:DMEL-XXX-02-015374-MON; -.
DMEL-XXX-02:DMEL-XXX-02-015375-MON; -.
DMEL-XXX-02:DMEL-XXX-02-015376-MON; -.
Organism-specific databases
FlyBase FBgn0002638; Bj1.
Gene expression databases
ArrayExpress P25171; -.
GermOnline CG10480; Drosophila melanogaster.
Ontologies
GO
GO:0005634; Cellular component: nucleus (inferred from direct assay from UniProtKB).
GO:0003682; Molecular function: chromatin binding (inferred from direct assay from UniProtKB).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0007417; Biological process: central nervous system development (inferred from direct assay from UniProtKB).
GO:0007346; Biological process: regulation of mitotic cell cycle (inferred from direct assay from UniProtKB).
GO:0050767; Biological process: regulation of neurogenesis (inferred from mutant phenotype from FlyBase).
GO:0046822; Biological process: regulation of nucleocytoplasmic transport (inferred from mutant phenotype from FlyBase).
QuickGo view.
Family and domain databases
InterPro IPR000408; Reg_chr_condens.
IPR009091; Reg_csome_cond/b-lactamase_inh.
Graphical view of domain structure.
Gene3D G3DSA:2.130.10.30; Reg_csome_cond/b-lactamase_inh; 1.
Pfam PF00415; RCC1; 5.
Pfam graphical view of domain structure.
PRINTS PR00633; RCCNDNSATION.
PROSITE PS00625; RCC1_1; 1.
PS00626; RCC1_2; 2.
PS50012; RCC1_3; 6.
PROSITE graphical view of domain structure (profiles).
BLOCKS P25171.
Genome annotation databases
Ensembl CG10480; Drosophila melanogaster. [Contig view]
GeneID 38669; -.
KEGG dme:Dmel_CG10480; -.
Phylogenomic databases
HOGENOM P25171; -.
Other
ProtoNet P25171.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cell cycle; Cell division; Complete proteome; Cytoplasm; Guanine-nucleotide releasing factor; Mitosis; Nucleus; Phosphoprotein; Repeat.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   547  547     Regulator of chromosome condensation. PRO_0000206633
REPEAT   41    91  51     RCC1 1. 
REPEAT   92   143  52     RCC1 2. 
REPEAT   145   195  51     RCC1 3. 
REPEAT   197   257  61     RCC1 4. 
REPEAT   260   310  51     RCC1 5. 
REPEAT   311   362  52     RCC1 6. 
REPEAT   363   416  54     RCC1 7. 
REPEAT   417   454  38     Bj1 1. 
REPEAT   455   489  35     Bj1 2. 
REPEAT   490   519  30     Bj1 3. 
COMPBIAS   520   547  28     Glu/Lys-rich. 
MOD_RES   286   286        Phosphothreonine. 
MOD_RES   512   512        Phosphothreonine. 
MOD_RES   526   526        Phosphoserine. 
MOD_RES   531   531        Phosphothreonine. 
CONFLICT   490   490        V -> A (in Ref. 1; CAA41417). 
Sequence information
Length: 547 AA [This is the length of the unprocessed precursor] Molecular weight: 58851 Da [This is the MW of the unprocessed precursor] CRC64: 77D9744EBAF4597A [This is a checksum on the sequence]
        10         20         30         40         50         60 
MPRRKALTNN NNAGEAEQQP PKAKRARIAF HLELPKRRTV LGNVLVCGNG DVGQLGLGED 

        70         80         90        100        110        120 
ILERKRLSPV AGIPDAVDIS AGGMHNLVLT KSGDIYSFGC NDEGALGRDT SEDGSESKPD 

       130        140        150        160        170        180 
LIDLPGKALC ISAGDSHSAC LLEDGRVFAW GSFRDSHGNM GLTIDGNKRT PIDLMEGTVC 

       190        200        210        220        230        240 
CSIASGADHL VILTTAGKVF TVGCAEQGQL GRLSERSISG EGRRGKRDLL RPTQLIITRA 

       250        260        270        280        290        300 
KPFEAIWATN YCTFMRESQT QVIWATGLNN FKQLAHETKG KEFALTPIKT ELKDIRHIAG 

       310        320        330        340        350        360 
GQHHTVILTT DLKCSVVGRP EYGRLGLGDV KDVVEKPTIV KKLTEKIVSV GCGEVCSYAV 

       370        380        390        400        410        420 
TIDGKLYSWG SGVNNQLGVG DGDDELEPIV VVSKNTQGKH MLLASGGGQH AIFLVKADKQ 

       430        440        450        460        470        480 
DQKENVPVKV SGSSSISKKD KTPPQDNVDK EAENVDKQEQ KENLPAKAST SSKKNKTPPQ 

       490        500        510        520        530        540 
DNADKEAENV DKQEQKENLP AKASTSSKKI KTPPQDDAAE EVEEESAQEP TPKKAKKPAA 


KRGGKKT 

P25171 in FASTA format

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