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UniProtKB/Swiss-Prot entry P25087


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ERG6_YEAST
Primary accession number P25087
Secondary accession numbers None
Integrated into Swiss-Prot on May 1, 1992
Sequence was last modified on January 23, 2007 (Sequence version 4)
Annotations were last modified on    November 25, 2008 (Entry version 84)
Name and origin of the protein
Protein name Sterol 24-C-methyltransferase
Synonyms EC 2.1.1.41
Delta(24)-sterol C-methyltransferase
Gene name
Name: ERG6
Synonyms: ISE1, LIS1, SED6
OrderedLocusNames: YML008C
ORFNames: YM9571.10C
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=8203167 [NCBI, ExPASy, EBI, Israel, Japan]
Hardwick K.G., Pelham H.R.B.;
"SED6 is identical to ERG6, and encodes a putative methyltransferase required for ergosterol synthesis.";
Yeast 10:265-269(1994).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0005-2736(94)90339-5; PubMed=8038180 [NCBI, ExPASy, EBI, Israel, Japan]
Welihinda A.A., Beavis A.D., Trumbly R.J.;
"Mutations in LIS1 (ERG6) gene confer increased sodium and lithium uptake in Saccharomyces cerevisiae.";
Biochim. Biophys. Acta 1193:107-117(1994).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=9169872 [NCBI, ExPASy, EBI, Israel, Japan]
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P., Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
Nature 387:90-93(1997).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-258.
DOI=10.1016/0378-1119(91)90238-7; PubMed=2060792 [NCBI, ExPASy, EBI, Israel, Japan]
Hussain M., Lenard J.;
"Characterization of PDR4, a Saccharomyces cerevisiae gene that confers pleiotropic drug resistance in high-copy number: identity with YAP1, encoding a transcriptional activator.";
Gene 101:149-152(1991).
[5]
ACETYLATION AT SER-2.
DOI=10.1002/elps.1150180810; PubMed=9298649 [NCBI, ExPASy, EBI, Israel, Japan]
Garrels J.I., McLaughlin C.S., Warner J.R., Futcher B., Latter G.I., Kobayashi R., Schwender B., Volpe T., Anderson D.S., Mesquita-Fuentes R., Payne W.E.;
"Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins.";
Electrophoresis 18:1347-1360(1997).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-375 AND SER-378, AND MASS SPECTROMETRY.
DOI=10.1038/nbt0302-301; PubMed=11875433 [NCBI, ExPASy, EBI, Israel, Japan]
Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M.;
"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae.";
Nat. Biotechnol. 20:301-305(2002).
[7]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[8]
INTERACTION WITH ERG28.
DOI=10.1016/j.bbalip.2004.08.001; PubMed=15522820 [NCBI, ExPASy, EBI, Israel, Japan]
Mo C., Valachovic M., Bard M.;
"The ERG28-encoded protein, Erg28p, interacts with both the sterol C-4 demethylation enzyme complex as well as the late biosynthetic protein, the C-24 sterol methyltransferase (Erg6p).";
Biochim. Biophys. Acta 1686:30-36(2004).
[9]
INTERACTION WITH ERG28.
DOI=10.1194/jlr.M500153-JLR200; PubMed=15995173 [NCBI, ExPASy, EBI, Israel, Japan]
Mo C., Bard M.;
"Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex.";
J. Lipid Res. 46:1991-1998(2005).
[10]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99 AND THR-373, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X74249; CAA52308.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S72460; AAB31378.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z49810; CAA89944.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X53830; CAA37826.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S42003; S42003.
RefSeq NP_013706.1; -.
3D structure databases
ModBase P25087.
Protein-protein interaction databases
DIP DIP:3816N; -.
IntAct P25087; -.
2D gel databases
SWISS-2DPAGE P25087; -.
Organism-specific databases
CYGD YML008c; -.
SGD S000004467; ERG6.
Yeast-GFP YML008C.
Gene expression databases
GermOnline YML008C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005811; Cellular component: lipid particle (inferred from direct assay from SGD).
GO:0005741; Cellular component: mitochondrial outer membrane (inferred from direct assay from SGD).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0003838; Molecular function: sterol 24-C-methyltransferase activity (inferred from electronic annotation from EC).
GO:0016126; Biological process: sterol biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR013216; Methyltransf_11.
IPR013705; Sterol_MeTrfase_C.
Graphical view of domain structure.
Pfam PF08241; Methyltransf_11; 1.
PF08498; Sterol_MT_C; 1.
Pfam graphical view of domain structure.
BLOCKS P25087.
ProtoNet P25087.
Proteomic databases
PeptideAtlas P25087; -.
Genome annotation databases
Ensembl YML008C; Saccharomyces cerevisiae. [Contig view]
GeneID 855003; -.
GenomeReviews Z71257_GR; YML008C.
KEGG sce:YML008C; -.
NMPDR fig|4932.3.peg.4748; -.
Phylogenomic databases
HOGENOM P25087; -.
Other
LinkHub P25087; -.
NextBio 978157; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Acetylation; Complete proteome; Lipid synthesis; Methyltransferase; Phosphoprotein; S-adenosyl-L-methionine; Steroid biosynthesis; Sterol biosynthesis; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   383  382     Sterol 24-C-methyltransferase. PRO_0000124799
MOD_RES   2     2        N-acetylserine. 
MOD_RES   99    99        Phosphoserine. 
MOD_RES   373   373        Phosphothreonine. 
MOD_RES   375   375        Phosphoserine. 
MOD_RES   378   378        Phosphoserine. 
CONFLICT   380   380        E -> EE (in Ref. 2; AAB31378). 
Sequence information
Length: 383 AA [This is the length of the unprocessed precursor] Molecular weight: 43431 Da [This is the MW of the unprocessed precursor] CRC64: C3A2B6DF2C14ED63 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSETELRKRQ AQFTRELHGD DIGKKTGLSA LMSKNNSAQK EAVQKYLRNW DGRTDKDAEE 

        70         80         90        100        110        120 
RRLEDYNEAT HSYYNVVTDF YEYGWGSSFH FSRFYKGESF AASIARHEHY LAYKAGIQRG 

       130        140        150        160        170        180 
DLVLDVGCGV GGPAREIARF TGCNVIGLNN NDYQIAKAKY YAKKYNLSDQ MDFVKGDFMK 

       190        200        210        220        230        240 
MDFEENTFDK VYAIEATCHA PKLEGVYSEI YKVLKPGGTF AVYEWVMTDK YDENNPEHRK 

       250        260        270        280        290        300 
IAYEIELGDG IPKMFHVDVA RKALKNCGFE VLVSEDLADN DDEIPWYYPL TGEWKYVQNL 

       310        320        330        340        350        360 
ANLATFFRTS YLGRQFTTAM VTVMEKLGLA PEGSKEVTAA LENAAVGLVA GGKSKLFTPM 

       370        380 
MLFVARKPEN AETPSQTSQE ATQ 

P25087 in FASTA format

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