NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=2167927 [NCBI, ExPASy, EBI, Israel, Japan]
Coulston J., Naif H., Brandon R., Kumar S., Khan S., Daniel R.C.W., Lavin M.F.;
"Molecular cloning and sequencing of an Australian isolate of proviral bovine leukaemia virus DNA: comparison with other isolates.";
J. Gen. Virol. 71:1737-1746(1990).

Comments

FUNCTION: During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.
CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-phosphomonoester.
PTM: Specific enzymatic cleavages in vivo yield mature proteins.
SIMILARITY: Belongs to the retroviral Pol polyprotein family.
SIMILARITY: Contains 1 integrase catalytic domain.
SIMILARITY: Contains 1 integrase-type DNA-binding domain.
SIMILARITY: Contains 1 reverse transcriptase domain.
SIMILARITY: Contains 1 RNase H domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

D00647; BAA00544.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

JQ0555; GNLJGA.

3D structure databases

HSSP

P03354; 1C1A. [HSSP ENTRY / PDB]

ModBase

P25059.

Ontologies

GO:0003677;	Molecular function: DNA binding (inferred from electronic annotation from InterPro).
GO:0003887;	Molecular function: DNA-directed DNA polymerase activity (inferred from electronic annotation from EC).
GO:0008907;	Molecular function: integrase activity (inferred from electronic annotation from InterPro).
GO:0004523;	Molecular function: ribonuclease H activity (inferred from electronic annotation from InterPro).
GO:0003723;	Molecular function: RNA binding (inferred from electronic annotation from InterPro).
GO:0003964;	Molecular function: RNA-directed DNA polymerase activity (inferred from electronic annotation from InterPro).
GO:0015074;	Biological process: DNA integration (inferred from electronic annotation from InterPro).
GO:0006310;	Biological process: DNA recombination (inferred from electronic annotation from UniProtKB-KW).
GO:0006278;	Biological process: RNA-dependent DNA replication (inferred from electronic annotation from InterPro).
GO:0032196;	Biological process: transposition (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR000477; DNA_pol_RVTase.
IPR001037; Integrase_C_retrovir.
IPR001584; Integrase_cat-core.
IPR002156; RNase_H.
IPR010661; RVT_thumb.
Graphical view of domain structure.

Pfam

PF00552; Integrase; 1.
PF00075; RnaseH; 1.
PF00665; rve; 1.
PF00078; RVT_1; 1.
PF06817; RVT_thumb; 1.
Pfam graphical view of domain structure.

PROSITE

PS50994; INTEGRASE; 1.
PS51027; INTEGRASE_DBD; 1.
PS50879; RNASE_H; 1.
PS50878; RT_POL; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

DNA integration; DNA recombination; Endonuclease; Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase; RNA-directed DNA polymerase; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 852`	`852`	`Pol polyprotein.`	`PRO_0000125481`
`DOMAIN`	`22 212`	`191`	`Reverse transcriptase.`
`DOMAIN`	`432 562`	`131`	`RNase H.`
`DOMAIN`	`615 779`	`165`	`Integrase catalytic.`
`DNA_BIND`	`788 836`	`49`	`Integrase-type.`

Sequence information

Length: 852 AA [This is the length of the unprocessed precursor]

Molecular weight: 95441 Da [This is the MW of the unprocessed precursor]

CRC64: CD69FE59E1208A08 [This is a checksum on the sequence]

        10         20         30         40         50         60 
GTSIPFKLER LQALQDLVHR SLEAGYISPW DGPGNNPVFP VRKPNGAWRF VHDLRVTNAL 

        70         80         90        100        110        120 
TKPIPALSPG PPDLTAIPTH LPHIICLDLK DAFFQIPVED RFRSYFAFTL PTPGGLQPHR 

       130        140        150        160        170        180 
RFAWRVLPQG FINSPALFER ALQEPLRQVS AAFSQSLLVS YMDDILYVSP TEEQRLQCYQ 

       190        200        210        220        230        240 
TMAAHLRDLG FQVASEKTRQ TPSPVPFLGQ MVHERMVTYQ SLPTLQISSP ISLHQLQTVL 

       250        260        270        280        290        300 
GDLQWVSRGT PTTRRPLQLL YSSLKGIDDP RAIIHLSPEQ QQGIAELRQA LSHNARSRYN 

       310        320        330        340        350        360 
EQEPLLAYVH LTRAGSTLVL FQKGAQFPLA YFQTPLTDNQ ASPWGLLLLL GCQYLQAQAL 

       370        380        390        400        410        420 
SSYAKTILKY YHNLPKTSLD NWIQSSEDPR VQELLQLWPQ ISSQGIQPPG PWKTLVTRAE 

       430        440        450        460        470        480 
VFLTPQFSPE PIPAALCLFS DGAARRGAYC LWKDHLLDFQ AVPAPESAQK GELAGLLAGL 

       490        500        510        520        530        540 
AAAPPEPLNI WVDSKYLYSL LRTLVLGAWL QPDPVPSYAL LYKSLLRHPA IFVGHVRSHS 

       550        560        570        580        590        600 
SASHPIASLN NYVDQLLPLE TPEQWHKLTH CNSRALSRWP NPRISAWDPR SPATLCETCQ 

       610        620        630        640        650        660 
RLNPTGGGKM RTIQRGWAPN HIWQADITHY KYKQFTYALH VFVDTYSGAT HASAKRGLTT 

       670        680        690        700        710        720 
QTTIEGLLEA IVHLGRPKKL NTDQGANYTS KTFVRFCQQF GISLSHHVPY NPTSSGLVER 

       730        740        750        760        770        780 
TNGLLKLLLS KYHLDEPHLP MTQALSRALW THNQINLLPI LKTRWELHHS PPLAVISEGG 

       790        800        810        820        830        840 
ETPKGSDKLF LYKLPGQNNR RWLGPLPALV EASGGALLAT NPPVWVPWRL LKAFKCLKND 

       850 
GPEDAPNRSS DG

P25059 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools