[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. DOI=10.1016/0888-7543(90)90042-S; PubMed=2286375 [NCBI, ExPASy, EBI, Israel, Japan] Manes T., Glade K., Ziomek C.A., Millan J.L.; "Genomic structure and comparison of mouse tissue-specific alkaline phosphatase genes."; Genomics 8:541-554(1990).

Comments

CATALYTIC ACTIVITY: A phosphate monoester + H₂O = an alcohol + phosphate.
COFACTOR: Binds 1 magnesium ion (By similarity).
COFACTOR: Binds 2 zinc ions (By similarity).
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
TISSUE SPECIFICITY: Embryo and testis.
MISCELLANEOUS: In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).
SIMILARITY: Belongs to the alkaline phosphatase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M61704; AAA37531.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

UniGene

Mm.195224

3D structure databases

HSSP

P05187; 1EW2. [HSSP ENTRY / PDB]

P24823; 19-497.

PTM databases

P24823; -.

Organism-specific databases

MGI

MGI:108009; Akp5.

Gene expression databases

ArrayExpress

P24823; -.

CleanEx

MM_AKP5; -.

GermOnline

ENSMUSG00000026246; Mus musculus.

Ontologies

GO:0031225;	Cellular component: anchored to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0004035;	Molecular function: alkaline phosphatase activity (inferred from direct assay from MGI).
GO:0000287;	Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008270;	Molecular function: zinc ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008152;	Biological process: metabolic process (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR017849; Alkaline_Pase-like_a/b/a.
IPR001952; Alkaline_phosphatase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.720.10; Alk_phosphtse; 1.

Pfam

PF00245; Alk_phosphatase; 1.
Pfam graphical view of domain structure.

PRINTS

PR00113; ALKPHPHTASE.

SMART

SM00098; alkPPc; 1.
SMART graphical view of domain structure.

PROSITE

PS00123; ALKALINE_PHOSPHATASE; 1.

ProtoNet

P24823.

Genome annotation databases

Ensembl

ENSMUSG00000026246; Mus musculus. [Contig view]

Phylogenomic databases

HOGENOM

P24823; -.

HOVERGEN

P24823; -.

Other

SOURCE

Akp5; Mus musculus.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Glycoprotein; GPI-anchor; Hydrolase; Lipoprotein; Magnesium; Membrane; Metal-binding; Phosphoprotein; Signal; Transmembrane; Zinc.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 18`	`18`	`Potential.`
`CHAIN`	`19 502`	`484`	`Embryonic alkaline phosphatase.`	`PRO_0000024045`
`PROPEP`	`503 529`	`27`	`Removed in mature form (By similarity).`	`PRO_0000024046`
`ACT_SITE`	`110 110`		`Phosphoserine intermediate.`
`METAL`	`60 60`		`Magnesium (Potential).`
`METAL`	`60 60`		`Zinc 2 (Potential).`
`METAL`	`329 329`		`Magnesium (Potential).`
`METAL`	`334 334`		`Zinc 1 (Potential).`
`METAL`	`338 338`		`Zinc 1 (Potential).`
`METAL`	`375 375`		`Zinc 2 (Potential).`
`METAL`	`376 376`		`Zinc 2 (Potential).`
`METAL`	`450 450`		`Zinc 1 (Potential).`
`LIPID`	`502 502`		`GPI-anchor amidated serine (By similarity).`
`CARBOHYD`	`140 140`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`267 267`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`277 277`		`N-linked (GlcNAc...) (Potential).`
`DISULFID`	`139 201`		`By similarity.`
`DISULFID`	`485 492`		`By similarity.`

Sequence information

Length: 529 AA [This is the length of the unprocessed precursor]

Molecular weight: 57212 Da [This is the MW of the unprocessed precursor]

CRC64: 2E0CD655AD4B30BA [This is a checksum on the sequence]

        10         20         30         40         50         60 
MWGACLLLLG LSLQVCPSVI PVEEENPAFW NRKAAEALDA AKKLKPIQTS AKNLVILMGD 

        70         80         90        100        110        120 
GMGVSTVTAT RILKGQQQGL SGPETQLAMD RFPHMALSKT YNTDKQIPDS AGTGTAFLCG 

       130        140        150        160        170        180 
VKTNMKVIGL SAAARFNQCN TTWGNEVVSV MHRAKKAGKS VGVVTTTSVQ HASPAGTYAH 

       190        200        210        220        230        240 
TVNRGWYSDA QMPASALQDG CKDISTQLIS NMDIDVILGG GRKFMFPKGT PDQEYPTDTK 

       250        260        270        280        290        300 
QAGTRLDGRN LVQEWLAKHQ GARYVWNRSE LIQASLNRSV THLMGLFEPN DMKYEIHRDP 

       310        320        330        340        350        360 
AQDPSLAEMT EVAVRMLSRN PKGFYLFVEG GRIDHGHHET VAYRALTEAV MFDSAVDKAD 

       370        380        390        400        410        420 
IRTSEQDTMI LVTADHSHVF SFGGYTQRGA SIFGLAPFKA EDGKSFTSIL YGNGPGYKLH 

       430        440        450        460        470        480 
NGARADVTEE ESSNPTYQQQ ACVPLSSETH SGEDVAIFAR GPQAHLVHGV QEQNYIAHVM 

       490        500        510        520 
AFAACLEPYT DCGLASPAGQ SSAVSPGYMS TLLCLLAGKM LMLMAAAEP

P24823 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools