ID   3BHS1_MOUSE             Reviewed;         373 AA.
AC   P24815; Q7TQ00;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-NOV-2008, entry version 68.
DE   RecName: Full=3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1;
DE            Short=3-beta-HSD I;
DE   Includes:
DE     RecName: Full=3-beta-hydroxy-Delta(5)-steroid dehydrogenase;
DE              EC=1.1.1.145;
DE     AltName: Full=3-beta-hydroxy-5-ene steroid dehydrogenase;
DE     AltName: Full=Progesterone reductase;
DE   Includes:
DE     RecName: Full=Steroid Delta-isomerase;
DE              EC=5.3.3.1;
DE     AltName: Full=Delta-5-3-ketosteroid isomerase;
GN   Name=Hsd3b1; Synonyms=Hsd3b;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=CD-1;
RX   MEDLINE=92020952; PubMed=1924345;
RA   Bain P.A., Yoo M., Clarke T., Hammond S.H., Payne A.H.;
RT   "Multiple forms of mouse 3 beta-hydroxysteroid dehydrogenase/delta 5-
RT   delta 4 isomerase and differential expression in gonads, adrenal
RT   glands, liver, and kidneys of both sexes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:8870-8874(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Egg;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: 3-beta-HSD is a bifunctional enzyme, that catalyzes the
CC       oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and
CC       the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic
CC       system plays a crucial role in the biosynthesis of all classes of
CC       hormonal steroids.
CC   -!- CATALYTIC ACTIVITY: A 3-beta-hydroxy-Delta(5)-steroid + NAD(+) = a
CC       3-oxo-Delta(5)-steroid + NADH.
CC   -!- CATALYTIC ACTIVITY: A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-
CC       steroid.
CC   -!- PATHWAY: Lipid metabolism; steroid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC       membrane protein. Mitochondrion membrane; Single-pass membrane
CC       protein.
CC   -!- TISSUE SPECIFICITY: Steroidogenic tissues (includes testes,
CC       ovaries and adrenal glands).
CC   -!- SIMILARITY: Belongs to the 3-beta-HSD family.
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DR   EMBL; M58567; AAA37860.1; -; mRNA.
DR   EMBL; BC052659; AAH52659.1; -; mRNA.
DR   PIR; I49762; I49762.
DR   RefSeq; NP_032319.1; -.
DR   UniGene; Mm.140811; -.
DR   Ensembl; ENSMUSG00000027871; Mus musculus.
DR   GeneID; 15492; -.
DR   MGI; MGI:96233; Hsd3b1.
DR   HOVERGEN; P24815; -.
DR   NextBio; 288366; -.
DR   ArrayExpress; P24815; -.
DR   GermOnline; ENSMUSG00000027871; Mus musculus.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0003854; F:3-beta-hydroxy-delta5-steroid dehydrogenase...; IEA:InterPro.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004769; F:steroid delta-isomerase activity; IEA:EC.
DR   GO; GO:0006700; P:C21-steroid hormone biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002225; 3Beta_OHSteriod_DHase/Estase.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF01073; 3Beta_HSD; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Isomerase; Membrane; Mitochondrion;
KW   Multifunctional enzyme; NAD; Oxidoreductase; Steroidogenesis;
KW   Transmembrane.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    373       3 beta-hydroxysteroid dehydrogenase/Delta
FT                                5-->4-isomerase type 1.
FT                                /FTId=PRO_0000087780.
FT   TRANSMEM    288    308       Potential.
FT   CONFLICT    128    128       V -> A (in Ref. 2; AAH52659).
FT   CONFLICT    319    319       L -> S (in Ref. 2; AAH52659).
SQ   SEQUENCE   373 AA;  42062 MW;  395F9D786C7BA010 CRC64;
     MAGWSCLVTG AGGFVGQRII KMLVQEKELQ EVRALDKVFR PETKEEFSKL QTKTKVTVLE
     GDILDAQCLR RACQGISVVI HTAAVIDVTG VIPRQTILDV NLKGTQNLLE ACVQASVPAF
     IFCSSVDVAG PNSYKKIVLN GHEEQNHEST WSDPYPYSKK MAEKAVLAAN GSMLKNGGTL
     NTCALRPMYI YGERSPFIFN AIIRALKNKG ILCVTGKFSI ANPVYVENVA WAHILAARGL
     RDPKKSTSIQ GQFYYISDDT PHQSYDDLNY TLSKEWGLRP NASWSLPLPL LYWLAFLLET
     VSFLLRPVYR YRPLFNRHLI TLSNSTFTFS YKKAQRDLGY EPLVNWEEAK QKTSEWIGTI
     VEQHREILDT KCQ
//