NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 23970 / CCUG 5853 / DSM 4691 / NCDC A7515 / NCTC 10617;
DOI=10.1007/BF00259456; PubMed=2277628 [NCBI, ExPASy, EBI, Israel, Japan]
Labbe D., Hoeltke H.J., Lau P.C.K.;
"Cloning and characterization of two tandemly arranged DNA methyltransferase genes of Neisseria lactamica: an adenine-specific M.NlaIII and a cytosine-type methylase.";
Mol. Gen. Genet. 224:101-110(1990).

Comments

FUNCTION: This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands.
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
SIMILARITY: Belongs to the C5-methyltransferase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X54485; CAA38357.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S12037; XYNHCL.

3D structure databases

HSSP

P05102; 6MHT. [HSSP ENTRY / PDB]

ModBase

P24581.

Protein family/group databases

REBASE

2158; M.NlaX.

Ontologies

GO:0003886;	Molecular function: DNA (cytosine-5-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0003677;	Molecular function: DNA binding (inferred from electronic annotation from InterPro).
GO:0006306;	Biological process: DNA methylation (inferred from electronic annotation from InterPro).
GO:0009307;	Biological process: DNA restriction-modification system (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001525; C5_DNA_meth.
Graphical view of domain structure.

PANTHER

PTHR10629; C5_DNA_meth; 1.

Pfam

PF00145; DNA_methylase; 1.
Pfam graphical view of domain structure.

PRINTS

PR00105; C5METTRFRASE.

TIGRFAMs

TIGR00675; dcm; 1.

PROSITE

PS00094; C5_MTASE_1; 1.
PS00095; C5_MTASE_2; 1.

ProtoNet

P24581.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Methyltransferase; Restriction system; S-adenosyl-L-methionine; Transferase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 313`	`313`	`Cytosine-specific methyltransferase NlaX.`	`PRO_0000087911`
`ACT_SITE`	`74 74`		`By similarity.`

Sequence information

Length: 313 AA [This is the length of the unprocessed precursor]

Molecular weight: 34843 Da [This is the MW of the unprocessed precursor]

CRC64: E75150C89ABEA611 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MFKIIDLFAG IGGIRLGFEQ AFDDVRCVFS SEIDKYAVQT YQANHGGETV CGDITQTDVA 

        70         80         90        100        110        120 
DIPDHDILSA GFPCQPFSQA GLKKGFADTR GTLFFDIERI LLAKKPQAFL LENVKQLKGH 

       130        140        150        160        170        180 
DKGRTLQVIL AHLQQAGYKV YTEVLKARDF GIPQNRERIY LVGFLNHDVD FRFPQPIGQA 

       190        200        210        220        230        240 
TAVGDILEAY PDEKYTISDK LWQGYQRRKA ENRAAGKGFG YGLFNAESAY TNTISARYYK 

       250        260        270        280        290        300 
DGSEILIEQP GKNPRKITPP EAARLQGFPD SFQIPVSDAQ AYRQFGNSVC VPVIRAIAEQ 

       310 
MKAALSAVSD RKV

P24581 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools