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UniProtKB/Swiss-Prot entry P24555


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PTRB_ECOLI
Primary accession number P24555
Secondary accession number P78068
Integrated into Swiss-Prot on March 1, 1992
Sequence was last modified on November 1, 1997 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 70)
Name and origin of the protein
Protein name Protease 2
Synonyms EC 3.4.21.83
Protease II
Oligopeptidase B
Gene name
Name: ptrB
Synonyms: tlp
OrderedLocusNames: b1845, JW1834
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
STRAIN=ATCC 33694 / HB101;
PubMed=1769955 [NCBI, ExPASy, EBI, Israel, Japan]
Kanatani A., Masuda T., Shimoda T., Misoka F., Lin X.S., Yoshimoto T., Tsuru D.;
"Protease II from Escherichia coli: sequencing and expression of the enzyme gene and characterization of the expressed enzyme.";
J. Biochem. 110:315-320(1991).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1093/dnares/3.6.379; PubMed=9097040 [NCBI, ExPASy, EBI, Israel, Japan]
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.;
"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.";
DNA Res. 3:379-392(1996).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
D10976; BAA01750.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC74915.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAA15651.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR E64946; E64946.
RefSeq AP_002465.1; -.
NP_416359.1; -.
3D structure databases
ModBase P24555.
Protein family/group databases
MEROPS S09.010; -.
Enzyme and pathway databases
BioCyc EcoCyc:EG11004-MON; -.
Organism-specific databases
EchoBASE EB0997; -.
EcoGene EG11004; ptrB.
Ontologies
GO
GO:0004252; Molecular function: serine-type endopeptidase activity (inferred from electronic annotation from InterPro).
GO:0006508; Biological process: proteolysis (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR002471; Pept_S9_AS.
IPR001375; Peptidase_S9.
IPR002470; Peptidase_S9A.
IPR004106; Peptidase_S9A_N.
Graphical view of domain structure.
PANTHER PTHR11757; Peptidase_S9A; 1.
Pfam PF00326; Peptidase_S9; 1.
PF02897; Peptidase_S9_N; 1.
Pfam graphical view of domain structure.
PRINTS PR00862; PROLIGOPTASE.
PROSITE PS00708; PRO_ENDOPEP_SER; 1.
BLOCKS P24555.
ProtoNet P24555.
Genome annotation databases
GeneID 946358; -.
GenomeReviews U00096_GR; b1845.
AP009048_GR; JW1834.
KEGG ecj:JW1834; -.
eco:b1845; -.
Phylogenomic databases
HOGENOM P24555; -.
Genome annotation databases
CMR P24555; b1845.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Direct protein sequencing; Hydrolase; Protease; Serine protease.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   686  686     Protease 2. PRO_0000122404
ACT_SITE   532   532        Charge relay system. 
ACT_SITE   617   617        Charge relay system (By similarity). 
ACT_SITE   652   652        Charge relay system (By similarity). 
CONFLICT   682   686        ATPAD -> LRLRTKYFPDNVSVLNAAPGSCCPGY (in Ref. 1; AA sequence). 
Sequence information
Length: 686 AA [This is the length of the unprocessed precursor] Molecular weight: 79491 Da [This is the MW of the unprocessed precursor] CRC64: B41AD388044D56B2 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MLPKAARIPH AMTLHGDTRI DNYYWLRDDT RSQPEVLDYL QQENSYGHRV MASQQALQDR 

        70         80         90        100        110        120 
ILKEIIDRIP QREVSAPYIK NGYRYRHIYE PGCEYAIYQR QSAFSEEWDE WETLLDANKR 

       130        140        150        160        170        180 
AAHSEFYSMG GMAITPDNTI MALAEDFLSR RQYGIRFRNL ETGNWYPELL DNVEPSFVWA 

       190        200        210        220        230        240 
NDSWIFYYVR KHPVTLLPYQ VWRHAIGTPA SQDKLIYEEK DDTYYVSLHK TTSKHYVVIH 

       250        260        270        280        290        300 
LASATTSEVR LLDAEMADAE PFVFLPRRKD HEYSLDHYQH RFYLRSNRHG KNFGLYRTRM 

       310        320        330        340        350        360 
RDEQQWEELI PPRENIMLEG FTLFTDWLVV EERQRGLTSL RQINRKTREV IGIAFDDPAY 

       370        380        390        400        410        420 
VTWIAYNPEP ETARLRYGYS SMTTPDTLFE LDMDTGERRV LKQTEVPGFY AANYRSEHLW 

       430        440        450        460        470        480 
IVARDGVEVP VSLVYHRKHF RKGHNPLLVY GYGSYGASID ADFSFSRLSL LDRGFVYAIV 

       490        500        510        520        530        540 
HVRGGGELGQ QWYEDGKFLK KKNTFNDYLD ACDALLKLGY GSPSLCYAMG GSAGGMLMGV 

       550        560        570        580        590        600 
AINQRPELFH GVIAQVPFVD VVTTMLDESI PLTTGEFEEW GNPQDPQYYE YMKSYSPYDN 

       610        620        630        640        650        660 
VTAQAYPHLL VTTGLHDSQV QYWEPAKWVA KLRELKTDDH LLLLCTDMDS GHGGKSGRFK 

       670        680 
SYEGVAMEYA FLVALAQGTL PATPAD 

P24555 in FASTA format

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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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