Lactose permease IIC component
     (PTS system lactose-specific EIIC component)
Lactose-specific phosphotransferase enzyme IIB component
     (EC 2.7.1.69)
     (PTS system lactose-specific EIIB component)

Gene name

Name:

lacE

From

Lactobacillus casei

[TaxID: 1582]

Taxonomy

Bacteria; Firmicutes; Lactobacillales; Lactobacillaceae; Lactobacillus.

Protein existence

4: Predicted;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=2125053 [NCBI, ExPASy, EBI, Israel, Japan]
Alpert C.-A., Chassy B.M.;
"Molecular cloning and DNA sequence of lacE, the gene encoding the lactose-specific enzyme II of the phosphotransferase system of Lactobacillus casei. Evidence that a cysteine residue is essential for sugar phosphorylation.";
J. Biol. Chem. 265:22561-22568(1990).

Comments

FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in lactose transport.
CATALYTIC ACTIVITY: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (Probable).
DOMAIN: The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
SIMILARITY: Contains 1 PTS EIIB type-3 domain.
SIMILARITY: Contains 1 PTS EIIC type-3 domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M60851; AAA72984.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

B23697; B23697.

3D structure databases

ModBase

P24400.

Ontologies

GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from InterPro).
GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0016301;	Molecular function: kinase activity (inferred from electronic annotation from UniProtKB-KW).
GO:0008982;	Molecular function: protein-N(PI)-phosphohistidine-sugar phosphotransferase activity (inferred from electronic annotation from InterPro).
GO:0005351;	Molecular function: sugar:hydrogen symporter activity (inferred from electronic annotation from InterPro).
GO:0009401;	Biological process: phosphoenolpyruvate-dependent sugar phosphotransferase system (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR013012; PTS_EIIB_3.
IPR014350; PTS_EIIB_3_sub.
IPR003352; PTS_EIIC.
IPR003501; PTS_IIB_lac.
IPR004501; PTS_IIC_lac.
IPR004801; PTS_IIC_lac_spec.
Graphical view of domain structure.

Pfam

PF02378; PTS_EIIC; 1.
PF02302; PTS_IIB; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00394; lac_pts_IIC; 1.
TIGR00410; lacE; 1.
TIGR00853; pts-lac; 1.

PROSITE

PS51100; PTS_EIIB_TYPE_3; 1.
PS51105; PTS_EIIC_TYPE_3; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Kinase; Membrane; Phosphotransferase system; Sugar transport; Transferase; Transmembrane; Transport.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 577`	`577`	`PTS system lactose-specific EIICB component.`	`PRO_0000186583`
`TRANSMEM`	`27 47`	`21`	`Potential.`
`TRANSMEM`	`63 83`	`21`	`Potential.`
`TRANSMEM`	`100 120`	`21`	`Potential.`
`TRANSMEM`	`133 153`	`21`	`Potential.`
`TRANSMEM`	`176 196`	`21`	`Potential.`
`TRANSMEM`	`219 239`	`21`	`Potential.`
`TRANSMEM`	`280 300`	`21`	`Potential.`
`TRANSMEM`	`326 346`	`21`	`Potential.`
`TRANSMEM`	`386 406`	`21`	`Potential.`
`DOMAIN`	`4 405`	`402`	`PTS EIIC type-3.`
`DOMAIN`	`476 577`	`102`	`PTS EIIB type-3.`
`ACT_SITE`	`483 483`		`Phosphocysteine intermediate; for EIIB activity (By similarity).`

Sequence information

Length: 577 AA [This is the length of the unprocessed precursor]

Molecular weight: 62392 Da [This is the MW of the unprocessed precursor]

CRC64: BE56852CAB0D76E4 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNKVFDKLKP VFEAIAANKY ISAIRDGFIA CMPIIIFSSI FMMVAYVPNA WGFYWPDNVT 

        70         80         90        100        110        120 
NTLMVAYNYS MGLLALFVAG TTAKNLTDSK NLELPKTNQI NPVAVIVASE ISFVILSILP 

       130        140        150        160        170        180 
LKTGVDLTYM GTQGLICAYI VGLIVPNIYY VCIKNNVTIK LPEQVPGNIA QSFKDLIPMG 

       190        200        210        220        230        240 
LSVTAFWLFG VGFKAATGTV LPRWIIQVLS PLFQASDSYL GLALIAGAMA FFWFCGVQGP 

       250        260        270        280        290        300 
SIVQPAVVPI MIANTAANLQ QYQAGQHVSH VLAMNTMDYV MNFGGTGATL VVPFIMLFAA 

       310        320        330        340        350        360 
RSAQLKAVGK AAFVPCTFGV NEPVLFGMPI IMNPMLFIPF LATPIVNVCL FKFFVSVLGM 

       370        380        390        400        410        420 
NSMMYTMPWT VPGPIGILIS TGFAPLAFVF VLLTLVLDVA IYFPFIRVYD STLLAEEKAK 

       430        440        450        460        470        480 
EEVIEDDGMA VLASDTVSPS IPTGLTVATA TDDDATHVLP ETAPSAHGEA YFKQNEVDVL 

       490        500        510        520        530        540 
VLCAGGGTSG ILANALNKLS KERGLKLSAA ARAYGQDMDL IKDMNMVILA PQMESMKGNL 

       550        560        570 
KKITDKYGVK LVTTTGRQYI ELTNNGDMAL DFVESNL

P24400 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools