[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT). PubMed=2476440 [NCBI, ExPASy, EBI, Israel, Japan] Andersson S., Bishop R.W., Russell D.W.; "Expression cloning and regulation of steroid 5 alpha-reductase, an enzyme essential for male sexual differentiation."; J. Biol. Chem. 264:16249-16255(1989).
[2]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG). TISSUE=Liver; DOI=10.1016/0167-4781(95)00198-0; PubMed=8597599 [NCBI, ExPASy, EBI, Israel, Japan] Lopez-Solache I., Luu-The V., Seralini G.E., Labrie F.; "Heterogeneity of rat type I 5 alpha-reductase cDNA: cloning, expression and regulation by pituitary implants and dihydrotestosterone."; Biochim. Biophys. Acta 1305:139-144(1996).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT). TISSUE=Brain; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).

Comments

FUNCTION: Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
CATALYTIC ACTIVITY: A 3-oxo-5-alpha-steroid + acceptor = a 3-oxo-Delta⁴-steroid + reduced acceptor.
BIOPHYSICOCHEMICAL PROPERTIES:

pH dependence: Optimally active at alkaline pHs;
SUBCELLULAR LOCATION: Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein (Potential).
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative initiation.

Name Long

Isoform ID P24008-1

This is the isoform sequence displayed in this entry.

Name Short

Isoform ID P24008-2

Features which should be applied to build the isoform sequence: VSP_018884.
TISSUE SPECIFICITY: Liver and prostate (at a low level).
DEVELOPMENTAL STAGE: After the establishment of chromosomal sex at fertilization.
INDUCTION: Its expression is regulated by androgens such as testosterone.
SIMILARITY: Belongs to the steroid 5-alpha reductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

J05035; AAA42102.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
S81448; AAB36218.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC127454; AAI27455.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

S65744; S65744.

NP_058766.2; -.

3D structure databases

ModBase

P24008.

Organism-specific databases

RGD

3757; Srd5a1.

Gene expression databases

ArrayExpress

P24008; -.

GermOnline

ENSRNOG00000017601; Rattus norvegicus.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from InterPro).
GO:0005792;	Cellular component: microsome (inferred from electronic annotation from UniProtKB-KW).
GO:0003865;	Molecular function: 3-oxo-5-alpha-steroid 4-dehydrogenase activity (inferred from electronic annotation from InterPro).
GO:0030154;	Biological process: cell differentiation (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0007530;	Biological process: sex determination (inferred from electronic annotation from UniProtKB-KW).
GO:0008202;	Biological process: steroid metabolic process (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR016636; 3-oxo-5-alpha-steroid_4-DH.
IPR001104; 3-oxo-5_a-steroid_4-DHase_C.
Graphical view of domain structure.

Pfam

PF02544; Steroid_dh; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF015596; 5_alpha-SR2; 1.

PROSITE

PS50244; S5A_REDUCTASE; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

P24008.

Genome annotation databases

Ensembl

ENSRNOG00000017601; Rattus norvegicus. [Contig view]

GeneID

24950; -.

KEGG

rno:24950; -.

Phylogenomic databases

HOVERGEN

P24008; -.

Other

NextBio

604965; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative initiation; Differentiation; Endoplasmic reticulum; Membrane; Microsome; Oxidoreductase; Sexual differentiation; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 259`	`259`	`3-oxo-5-alpha-steroid 4-dehydrogenase 1.`	`PRO_0000031565`
`TRANSMEM`	`10 30`	`21`	`Potential.`
`TRANSMEM`	`86 106`	`21`	`Potential.`
`TRANSMEM`	`111 131`	`21`	`Potential.`
`TRANSMEM`	`146 166`	`21`	`Potential.`
`TRANSMEM`	`206 226`	`21`	`Potential.`
`VAR_SEQ`	`1 4`		`Missing (in isoform Short).`	`VSP_018884`

Sequence information

Length: 259 AA [This is the length of the unprocessed precursor]

Molecular weight: 29780 Da [This is the MW of the unprocessed precursor]

CRC64: FE6705AC1D7CAE11 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVPLMELDEL CLLDMLVYLE GFMAFVSIVG LRSVGSPYGR YSPQWPGIRV PARPAWFIQE 

        70         80         90        100        110        120 
LPSMAWPLYE YIRPAAARLG NLPNRVLLAM FLIHYVQRTL VFPVLIRGGK PTLLVTFVLA 

       130        140        150        160        170        180 
FLFCTFNGYV QSRYLSQFAV YAEDWVTHPC FLTGFALWLV GMVINIHSDH ILRNLRKPGE 

       190        200        210        220        230        240 
TGYKIPRGGL FEYVSAANYF GELVEWCGFA LASWSLQGVV FALFTLSTLL TRAKQHHQWY 

       250 
HEKFEDYPKS RKILIPFVL

P24008 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools