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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
TISSUE=Kidney;
PubMed=9693127 [NCBI, ExPASy, EBI, Israel, Japan]
Mendelson I.,
Kronman C.,
Ariel N.,
Shafferman A.,
Velan B.;
"Bovine acetylcholinesterase: cloning, expression and characterization.";
Biochem. J. 334:251-259(1998).
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[2]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM T).
STRAIN=Hereford;
TISSUE=Basal ganglia;
NIH - Mammalian Gene Collection (MGC) project;
Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
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[3]
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PROTEIN SEQUENCE OF 31-613 (ISOFORM H).
TISSUE=Fetal serum;
DOI=10.1016/0014-5793(90)81522-P; PubMed=2365060 [NCBI, ExPASy, EBI, Israel, Japan]
Doctor B.P.,
Chapman T.C.,
Christner C.E.,
Deal C.D.,
de la Hoz D.M.,
Gentry M.K.,
Ogert R.A.,
Rush R.S.,
Smyth K.K.,
Wolfe A.D.;
"Complete amino acid sequence of fetal bovine serum acetylcholinesterase and its comparison in various regions with other cholinesterases.";
FEBS Lett. 266:123-127(1990).
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- FUNCTION: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
- CATALYTIC ACTIVITY: Acetylcholine + H2O = choline + acetate.
- SUBUNIT: Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers (By similarity). Isoform H generates GPI-anchored dimers; disulfide linked. Isoform T generates multiple structures, ranging from monomers and dimers to collagen-tailed and hydrophobic-tailed forms, in which catalytic tetramers are associated with anchoring proteins that attach them to the basal lamina or to cell membranes. In the collagen-tailed forms, isoform T subunits are associated with a specific collagen, COLQ, which triggers the formation of isoform T tetramers, from monomers and dimers.
- SUBCELLULAR LOCATION: Cell junction, synapse. Secreted. Cell membrane; Peripheral membrane protein (By similarity).
- SUBCELLULAR LOCATION: Isoform H: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.
- ALTERNATIVE PRODUCTS:
2 named isoforms [FASTA] produced by alternative splicing.
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| Name | H |
| Isoform ID | P23795-2 |
| Features which should be applied to build the isoform sequence: VSP_001455. |
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- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 613 AA [This is the length of the unprocessed precursor] |
Molecular weight: 67664 Da [This is the MW of the unprocessed precursor] |
CRC64: 698D4F0DF8624B12 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MRPPWCPLHT PSLTPPLLLL LFLIGGGAEA EGPEDPELLV MVRGGRLRGL RLMAPRGPVS
70 80 90 100 110 120
AFLGIPFAEP PVGPRRFLPP EPKRPWPGVL NATAFQSVCY QYVDTLYPGF EGTEMWNPNR
130 140 150 160 170 180
ELSEDCLYLN VWTPYPRPSS PTPVLVWIYG GGFYSGASSL DVYDGRFLTQ AEGTVLVSMN
190 200 210 220 230 240
YRVGAFGFLA LPGSREAPGN VGLLDQRLAL QWVQENVAAF GGDPTSVTLF GESAGAASVG
250 260 270 280 290 300
MHLLSPPSRG LFHRAVLQSG APNGPWATVG VGEARRRATL LARLVGCPPG GAGGNDTELV
310 320 330 340 350 360
ACLRARPAQD LVDHEWRVLP QESVFRFSFV PVVDGDFLSD TPEALINAGD FHGLQVLVGV
370 380 390 400 410 420
VKDEGSYFLV YGAPGFSKDN ESLISRAQFL AGVRVGVPQA SDLAAEAVVL HYTDWLHPED
430 440 450 460 470 480
PARLREALSD VVGDHNVVCP VAQLAGRLAA QGARVYAYIF EHRASTLSWP LWMGVPHGYE
490 500 510 520 530 540
IEFIFGLPLE PSLNYTIEER TFAQRLMRYW ANFARTGDPN DPRDPKAPQW PPYTAGAQQY
550 560 570 580 590 600
VSLNLRPLEV RRGLRAQACA FWNRFLPKLL SATDTLDEAE RQWKAEFHRW SSYMVHWKNQ
610
FDHYSKQDRC SDL
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P23795 in FASTA format |
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