[1]
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NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1016/0092-8674(91)90411-Q; PubMed=1707345 [NCBI, ExPASy, EBI, Israel, Japan]
Otsu M.,
Hiles I.D.,
Goot I.,
Fry M.J.,
Ruiz-Larrea F.,
Panayotou G.,
Thompson A.,
Dhand R.,
Hsuan J.,
Totty N.,
Smith A.D.,
Morgan S.J.,
Courtneidge S.A.,
Parker P.J.,
Waterfield M.D.;
"Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase.";
Cell 65:91-104(1991).
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[2]
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CIRCULAR DICHROISM ANALYSIS, AND FLUORESCENCE SPECTROSCOPY.
PubMed=1330535 [NCBI, ExPASy, EBI, Israel, Japan]
Panayotou G.,
Bax B.,
Gout I.,
Federwisch M.,
Wroblowski B.,
Dhand R.,
Fry M.J.,
Blundell T.L.,
Wollmer A.,
Waterfield M.D.;
"Interaction of the p85 subunit of PI 3-kinase and its N-terminal SH2 domain with a PDGF receptor phosphorylation site: structural features and analysis of conformational changes.";
EMBO J. 11:4261-4272(1992).
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[3]
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STRUCTURE BY NMR OF 1-84.
DOI=10.1016/0092-8674(93)90259-S; PubMed=7684655 [NCBI, ExPASy, EBI, Israel, Japan]
Booker G.W.,
Gout I.,
Downing A.K.,
Driscoll P.C.,
Boyd J.,
Waterfield M.D.,
Campbell I.D.;
"Solution structure and ligand-binding site of the SH3 domain of the p85 alpha subunit of phosphatidylinositol 3-kinase.";
Cell 73:813-822(1993).
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[4]
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STRUCTURE BY NMR OF 314-431.
DOI=10.1038/358684a0; PubMed=1323062 [NCBI, ExPASy, EBI, Israel, Japan]
Booker G.W.,
Breeze A.L.,
Downing A.K.,
Panayotou G.,
Gout I.,
Waterfield M.D.,
Campbell I.D.;
"Structure of an SH2 domain of the p85 alpha subunit of phosphatidylinositol-3-OH kinase.";
Nature 358:684-687(1992).
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[5]
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STRUCTURE BY NMR OF 321-434.
DOI=10.1021/bi961783x; PubMed=8952511 [NCBI, ExPASy, EBI, Israel, Japan]
Guenther U.L.,
Liu Y.,
Sanford D.,
Bachovchin W.W.,
Schaffhausen B.;
"NMR analysis of interactions of a phosphatidylinositol 3'-kinase SH2 domain with phosphotyrosine peptides reveals interdependence of major binding sites.";
Biochemistry 35:15570-15581(1996).
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[6]
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STRUCTURE BY NMR OF 614-724.
DOI=10.1006/jmbi.1997.1562; PubMed=9512716 [NCBI, ExPASy, EBI, Israel, Japan]
Siegal G.,
Davis B.,
Kristensen S.M.,
Sankar A.,
Linacre J.,
Stein R.C.,
Panayotou G.,
Waterfield M.D.,
Driscoll P.C.;
"Solution structure of the C-terminal SH2 domain of the p85 alpha regulatory subunit of phosphoinositide 3-kinase.";
J. Mol. Biol. 276:461-478(1998).
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- FUNCTION: Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
- SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated LAT, LAX1, TRAT1 and LIME1 upon TCR and/or BCR activation. Interacts with CBLB. The SH2 domains interact with the YTHM motif of phosphorylated INSR in vitro. Also interacts with tyrosine-phosphorylated IGF1R in vitro. Interacts with CD28 and CD3Z upon T-cell activation. Interacts with SOCS7. Interacts with IRS1 and phosphorylated IRS4. Interacts with NISCH, RUFY3 and HCST (By similarity).
- INTERACTION:
P14753:Epor (xeno); NbExp=1; IntAct=EBI-520244, EBI-617901;
Q9ES52-1:Inpp5d (xeno); NbExp=1; IntAct=EBI-520244, EBI-1452545;
Q9ES52-3:Inpp5d (xeno); NbExp=1; IntAct=EBI-520244, EBI-1452551;
P05622:Pdgfrb (xeno); NbExp=1; IntAct=EBI-520244, EBI-1554855;
P32871:PIK3CA; NbExp=2; IntAct=EBI-520244, EBI-1373130;
P42337:Pik3ca (xeno); NbExp=1; IntAct=EBI-520244, EBI-641748;
P20339:RAB5A (xeno); NbExp=2; IntAct=EBI-520244, EBI-399437;
- DOMAIN: The SH3 domain mediates the binding to CBLB (By similarity).
- PTM: Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation (By similarity).
- SIMILARITY: Belongs to the PI3K p85 subunit family.
- SIMILARITY: Contains 1 Rho-GAP domain.
- SIMILARITY: Contains 2 SH2 domains.
- SIMILARITY: Contains 1 SH3 domain.
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