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UniProtKB/Swiss-Prot entry P23710


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NDUS3_NEUCR
Primary accession number P23710
Secondary accession number Q7RV79
Integrated into Swiss-Prot on November 1, 1991
Sequence was last modified on June 7, 2005 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 65)
Name and origin of the protein
Protein name NADH-ubiquinone oxidoreductase 30.4 kDa subunit, mitochondrial [Precursor]
Synonyms EC 1.6.5.3
EC 1.6.99.3
Complex I-30kD
CI-31kD
Gene name
Name: nuo-31
ORFNames: NCU04074
From
Neurospora crassa [TaxID: 5141] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1016/0006-291X(90)90807-Y; PubMed=2145832 [NCBI, ExPASy, EBI, Israel, Japan]
Videira A., Tropschug M., Werner S.;
"Primary structure and expression of a nuclear-coded subunit of complex I homologous to proteins specified by the chloroplast genome.";
Biochem. Biophys. Res. Commun. 171:1168-1174(1990).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
DOI=10.1038/nature01554; PubMed=12712197 [NCBI, ExPASy, EBI, Israel, Japan]
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
"The genome sequence of the filamentous fungus Neurospora crassa.";
Nature 422:859-868(2003).
Comments
  • FUNCTION: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • CATALYTIC ACTIVITY: NADH + ubiquinone = NAD+ + ubiquinol.
  • CATALYTIC ACTIVITY: NADH + acceptor = NAD+ + reduced acceptor.
  • SUBUNIT: Complex I is composed of about 40 different subunits. This is a component of the iron-sulfur protein fraction.
  • SUBCELLULAR LOCATION: Mitochondrion inner membrane.
  • SIMILARITY: Belongs to the complex I 30 kDa subunit family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AABX02000012; EAA28453.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A35935; A35935.
RefSeq XP_957689.1; -.
3D structure databases
ModBase P23710.
Ontologies
GO
GO:0005746; Cellular component: mitochondrial respiratory chain (inferred from electronic annotation from UniProtKB-KW).
GO:0008137; Molecular function: NADH dehydrogenase (ubiquinone) activity (inferred from electronic annotation from InterPro).
GO:0006120; Biological process: mitochondrial electron transport, NADH to ubiquinone (inferred from electronic annotation from InterPro).
GO:0006810; Biological process: transport (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR010218; NADH_DH_csu.
IPR001268; NADH_DHase_Ub_30kDa_su.
Graphical view of domain structure.
Pfam PF00329; Complex1_30kDa; 1.
Pfam graphical view of domain structure.
ProDom PD001581; Complex1_30K; 1.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR01961; NuoC_fam; 1.
PROSITE PS00542; COMPLEX1_30K; 1.
ProtoNet P23710.
Genome annotation databases
GeneID 3873779; -.
KEGG ncr:NCU04074; -.
NMPDR fig|5141.1.peg.1326; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane; NAD; Oxidoreductase; Respiratory chain; Transit peptide; Transport; Ubiquinone.
Features
SEVIEWER logo Feature table viewer
KeyFrom  To Length Description FTId
TRANSIT   1    17  17     Mitochondrion (Potential). 
CHAIN   18   283  266     NADH-ubiquinone oxidoreductase 30.4 kDa subunit, mitochondrial. PRO_0000020000
CONFLICT   40    40        G -> R (in Ref. 1). 
Sequence information
Length: 283 AA [This is the length of the unprocessed precursor] Molecular weight: 32185 Da [This is the MW of the unprocessed precursor] CRC64: 3A2DCD3489F32C6F [This is a checksum on the sequence]
        10         20         30         40         50         60 
MASKLCRSRA LASALRSAKP SPAIRCLATT SRNLINMPEG PNPRQFPREP LPGALNAAVV 

        70         80         90        100        110        120 
NPADKYQSKA DNLHKYGSWL MGCLPKYIQQ FSVWKDELTI YISPAGVIPV FSFLKYNTAA 

       130        140        150        160        170        180 
EYTQVSDITA VDFPTKDQRF EVVYNLLSVR HNSRIRVKTY ADEVSPVPSI TPLYDGANWY 

       190        200        210        220        230        240 
EREVYDLFGV FFTGHPDLRR IMTDYGFDGH PLRKDFPMTG YTEIRYDEEK KRIVTEPLEM 

       250        260        270        280 
TQAFRNFEGG SSAWEQVGAG IDRKPESFKL PTPKPETKPE EKK 

P23710 in FASTA format

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